Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF997 and GFF998 are separated by 73 nucleotides GFF998 and GFF999 are separated by 68 nucleotides GFF999 and GFF1000 are separated by 26 nucleotides GFF1000 and GFF1001 are separated by 47 nucleotides
GFF997 - Putative inner membrane protein, at 179,624 to 180,325
GFF997
GFF998 - FIG00638399: hypothetical protein, at 180,399 to 180,731
GFF998
GFF999 - hypothetical protein, at 180,800 to 180,931
GFF999
GFF1000 - Type I restriction-modification system, specificity subunit S (EC 3.1.21.3), at 180,958 to 182,316
GFF1000
GFF1001 - Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72), at 182,364 to 183,953
GFF1001
Position (kb) 180
181
182
183 Strain fitness (log2 ratio) -1
0
1 at 180.018 kb on - strand, within GFF997 at 180.299 kb on - strand at 180.359 kb on + strand at 180.534 kb on - strand, within GFF998 at 180.631 kb on + strand, within GFF998 at 180.653 kb on - strand, within GFF998 at 180.714 kb on - strand at 180.726 kb on - strand at 181.860 kb on + strand, within GFF1000 at 181.917 kb on - strand, within GFF1000 at 182.234 kb on + strand at 182.774 kb on + strand, within GFF1001 at 182.821 kb on + strand, within GFF1001 at 183.170 kb on + strand, within GFF1001 at 183.170 kb on + strand, within GFF1001
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 180,018 - GFF997 0.56 -0.2 180,299 - +0.1 180,359 + +0.0 180,534 - GFF998 0.41 -0.2 180,631 + GFF998 0.70 -0.7 180,653 - GFF998 0.76 +0.6 180,714 - +0.0 180,726 - -0.8 181,860 + GFF1000 0.66 -0.1 181,917 - GFF1000 0.71 -1.7 182,234 + +1.0 182,774 + GFF1001 0.26 +0.1 182,821 + GFF1001 0.29 -0.4 183,170 + GFF1001 0.51 -0.3 183,170 + GFF1001 0.51 +0.1
Or see this region's nucleotide sequence