Experiment: Nicotinamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5114 and Atu5115 are separated by 3 nucleotides Atu5115 and Atu5116 are separated by 41 nucleotides Atu5116 and Atu5117 are separated by 311 nucleotides Atu5117 and Atu5118 are separated by 162 nucleotides
Atu5114: Atu5114 - hypothetical protein, at 122,694 to 123,026
Atu5114
Atu5115: Atu5115 - hypothetical protein, at 123,030 to 123,296
Atu5115
Atu5116: Atu5116 - transcriptional regulator protein, at 123,338 to 123,649
Atu5116
Atu5117: Atu5117 - hypothetical protein, at 123,961 to 124,902
Atu5117
Atu5118: Atu5118 - hypothetical protein, at 125,065 to 126,306
Atu5118
Position (kb)
123
124
125 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 123.043 kb on - strand at 123.052 kb on - strand at 123.068 kb on - strand, within Atu5115 at 123.249 kb on - strand, within Atu5115 at 123.269 kb on + strand, within Atu5115 at 123.277 kb on - strand at 123.308 kb on - strand at 123.319 kb on + strand at 123.329 kb on - strand at 123.360 kb on + strand at 123.399 kb on + strand, within Atu5116 at 123.399 kb on + strand, within Atu5116 at 123.442 kb on + strand, within Atu5116 at 123.450 kb on - strand, within Atu5116 at 123.459 kb on + strand, within Atu5116 at 123.480 kb on - strand, within Atu5116 at 123.491 kb on - strand, within Atu5116 at 123.505 kb on + strand, within Atu5116 at 123.505 kb on - strand, within Atu5116 at 123.569 kb on + strand, within Atu5116 at 123.574 kb on + strand, within Atu5116 at 123.574 kb on + strand, within Atu5116 at 123.577 kb on - strand, within Atu5116 at 123.577 kb on - strand, within Atu5116 at 123.582 kb on - strand, within Atu5116 at 123.582 kb on - strand, within Atu5116 at 123.584 kb on + strand, within Atu5116 at 123.587 kb on - strand, within Atu5116 at 123.685 kb on + strand at 123.685 kb on + strand at 123.829 kb on + strand at 123.916 kb on - strand at 124.049 kb on + strand at 124.062 kb on - strand, within Atu5117 at 124.121 kb on - strand, within Atu5117 at 124.121 kb on - strand, within Atu5117 at 124.121 kb on - strand, within Atu5117 at 124.151 kb on + strand, within Atu5117 at 124.168 kb on + strand, within Atu5117 at 124.193 kb on + strand, within Atu5117 at 124.267 kb on - strand, within Atu5117 at 124.267 kb on - strand, within Atu5117 at 124.284 kb on - strand, within Atu5117 at 124.334 kb on + strand, within Atu5117 at 124.437 kb on - strand, within Atu5117 at 124.451 kb on + strand, within Atu5117 at 124.451 kb on + strand, within Atu5117 at 124.451 kb on + strand, within Atu5117 at 124.451 kb on + strand, within Atu5117 at 124.451 kb on + strand, within Atu5117 at 124.459 kb on - strand, within Atu5117 at 124.504 kb on - strand, within Atu5117 at 124.576 kb on + strand, within Atu5117 at 124.591 kb on + strand, within Atu5117 at 124.608 kb on + strand, within Atu5117 at 124.656 kb on + strand, within Atu5117 at 124.702 kb on - strand, within Atu5117 at 124.702 kb on - strand, within Atu5117 at 124.807 kb on - strand, within Atu5117 at 124.817 kb on - strand at 124.934 kb on + strand at 125.054 kb on - strand at 125.084 kb on + strand at 125.100 kb on + strand at 125.100 kb on - strand at 125.103 kb on - strand at 125.110 kb on - strand at 125.120 kb on - strand at 125.124 kb on + strand at 125.129 kb on + strand at 125.137 kb on - strand at 125.173 kb on - strand at 125.387 kb on - strand, within Atu5118 at 125.464 kb on + strand, within Atu5118 at 125.464 kb on + strand, within Atu5118 at 125.548 kb on - strand, within Atu5118 at 125.592 kb on - strand, within Atu5118 at 125.619 kb on - strand, within Atu5118 at 125.678 kb on + strand, within Atu5118 at 125.678 kb on + strand, within Atu5118 at 125.686 kb on - strand, within Atu5118 at 125.699 kb on - strand, within Atu5118 at 125.749 kb on + strand, within Atu5118 at 125.757 kb on - strand, within Atu5118 at 125.765 kb on - strand, within Atu5118 at 125.785 kb on - strand, within Atu5118 at 125.797 kb on - strand, within Atu5118 at 125.830 kb on + strand, within Atu5118 at 125.882 kb on + strand, within Atu5118 at 125.886 kb on - strand, within Atu5118 at 125.900 kb on - strand, within Atu5118
Per-strain Table
Position Strand Gene LocusTag Fraction Nicotinamide (N) remove 123,043 - -0.2 123,052 - -0.1 123,068 - Atu5115 0.14 +0.9 123,249 - Atu5115 0.82 -0.5 123,269 + Atu5115 0.90 +2.0 123,277 - +0.2 123,308 - +1.5 123,319 + +0.8 123,329 - +0.4 123,360 + +0.0 123,399 + Atu5116 0.20 +0.1 123,399 + Atu5116 0.20 +2.6 123,442 + Atu5116 0.33 +1.1 123,450 - Atu5116 0.36 -0.7 123,459 + Atu5116 0.39 +0.0 123,480 - Atu5116 0.46 +1.6 123,491 - Atu5116 0.49 -0.3 123,505 + Atu5116 0.54 -2.0 123,505 - Atu5116 0.54 +0.7 123,569 + Atu5116 0.74 +1.9 123,574 + Atu5116 0.76 +3.8 123,574 + Atu5116 0.76 +0.3 123,577 - Atu5116 0.77 +0.5 123,577 - Atu5116 0.77 +0.8 123,582 - Atu5116 0.78 -0.3 123,582 - Atu5116 0.78 +1.8 123,584 + Atu5116 0.79 -0.4 123,587 - Atu5116 0.80 +0.4 123,685 + +0.6 123,685 + +0.6 123,829 + +0.3 123,916 - -0.3 124,049 + -0.7 124,062 - Atu5117 0.11 -0.4 124,121 - Atu5117 0.17 +0.5 124,121 - Atu5117 0.17 +2.2 124,121 - Atu5117 0.17 -0.0 124,151 + Atu5117 0.20 +0.3 124,168 + Atu5117 0.22 +1.8 124,193 + Atu5117 0.25 +0.9 124,267 - Atu5117 0.32 +0.3 124,267 - Atu5117 0.32 +1.7 124,284 - Atu5117 0.34 -1.4 124,334 + Atu5117 0.40 -1.1 124,437 - Atu5117 0.51 +0.0 124,451 + Atu5117 0.52 -0.0 124,451 + Atu5117 0.52 -0.0 124,451 + Atu5117 0.52 -1.6 124,451 + Atu5117 0.52 -0.2 124,451 + Atu5117 0.52 -1.3 124,459 - Atu5117 0.53 +0.5 124,504 - Atu5117 0.58 -0.3 124,576 + Atu5117 0.65 -0.9 124,591 + Atu5117 0.67 -0.6 124,608 + Atu5117 0.69 +5.2 124,656 + Atu5117 0.74 +0.4 124,702 - Atu5117 0.79 -0.2 124,702 - Atu5117 0.79 +1.0 124,807 - Atu5117 0.90 -0.3 124,817 - +3.2 124,934 + -0.3 125,054 - +0.6 125,084 + +0.6 125,100 + +0.2 125,100 - +2.8 125,103 - +1.6 125,110 - +1.1 125,120 - +1.0 125,124 + -0.4 125,129 + +1.8 125,137 - +2.2 125,173 - +0.1 125,387 - Atu5118 0.26 -0.5 125,464 + Atu5118 0.32 +0.3 125,464 + Atu5118 0.32 -0.2 125,548 - Atu5118 0.39 +1.0 125,592 - Atu5118 0.42 +3.6 125,619 - Atu5118 0.45 +0.8 125,678 + Atu5118 0.49 +2.2 125,678 + Atu5118 0.49 +0.3 125,686 - Atu5118 0.50 +0.0 125,699 - Atu5118 0.51 +1.8 125,749 + Atu5118 0.55 +0.8 125,757 - Atu5118 0.56 +0.3 125,765 - Atu5118 0.56 +1.3 125,785 - Atu5118 0.58 +1.2 125,797 - Atu5118 0.59 +0.9 125,830 + Atu5118 0.62 +1.3 125,882 + Atu5118 0.66 +1.5 125,886 - Atu5118 0.66 +1.0 125,900 - Atu5118 0.67 -0.2
Or see this region's nucleotide sequence