Experiment: Nicotinamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5071 and Atu8046 are separated by 77 nucleotides Atu8046 and Atu5072 are separated by 121 nucleotides Atu5072 and Atu5073 are separated by 3 nucleotides Atu5073 and Atu5074 overlap by 4 nucleotides
Atu5071: Atu5071 - ABC transporter substrate binding protein (dipeptide), at 70,032 to 71,549
Atu5071
Atu8046: Atu8046 - hypothetical protein, at 71,627 to 71,779
Atu8046
Atu5072: Atu5072 - hypothetical protein, at 71,901 to 73,091
Atu5072
Atu5073: Atu5073 - pyridoxal phosphate biosynthesis protein, at 73,095 to 74,060
Atu5073
Atu5074: Atu5074 - iron alcohol dehydrogenase, at 74,057 to 75,178
Atu5074
Position (kb)
71
72
73
74 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 70.908 kb on - strand, within Atu5071 at 70.986 kb on - strand, within Atu5071 at 70.988 kb on + strand, within Atu5071 at 71.040 kb on + strand, within Atu5071 at 71.077 kb on - strand, within Atu5071 at 71.113 kb on + strand, within Atu5071 at 71.169 kb on - strand, within Atu5071 at 71.257 kb on + strand, within Atu5071 at 71.267 kb on + strand, within Atu5071 at 71.267 kb on + strand, within Atu5071 at 71.286 kb on + strand, within Atu5071 at 71.323 kb on - strand, within Atu5071 at 71.353 kb on - strand, within Atu5071 at 71.353 kb on - strand, within Atu5071 at 71.395 kb on + strand, within Atu5071 at 71.411 kb on + strand at 71.473 kb on + strand at 71.473 kb on + strand at 71.481 kb on - strand at 71.481 kb on - strand at 71.481 kb on - strand at 71.522 kb on - strand at 71.552 kb on + strand at 71.553 kb on + strand at 71.561 kb on + strand at 71.635 kb on - strand at 71.668 kb on - strand, within Atu8046 at 71.681 kb on - strand, within Atu8046 at 71.694 kb on + strand, within Atu8046 at 71.706 kb on - strand, within Atu8046 at 71.913 kb on - strand at 71.963 kb on + strand at 71.981 kb on - strand at 72.014 kb on - strand at 72.014 kb on - strand at 72.018 kb on - strand at 72.041 kb on - strand, within Atu5072 at 72.151 kb on + strand, within Atu5072 at 72.170 kb on + strand, within Atu5072 at 72.227 kb on + strand, within Atu5072 at 72.270 kb on - strand, within Atu5072 at 72.291 kb on - strand, within Atu5072 at 72.291 kb on - strand, within Atu5072 at 72.292 kb on + strand, within Atu5072 at 72.292 kb on + strand, within Atu5072 at 72.296 kb on - strand, within Atu5072 at 72.318 kb on - strand, within Atu5072 at 72.387 kb on - strand, within Atu5072 at 72.438 kb on + strand, within Atu5072 at 72.482 kb on + strand, within Atu5072 at 72.490 kb on - strand, within Atu5072 at 72.490 kb on - strand, within Atu5072 at 72.507 kb on - strand, within Atu5072 at 72.686 kb on - strand, within Atu5072 at 72.783 kb on + strand, within Atu5072 at 72.837 kb on - strand, within Atu5072 at 72.899 kb on + strand, within Atu5072 at 72.904 kb on + strand, within Atu5072 at 72.912 kb on - strand, within Atu5072 at 72.912 kb on - strand, within Atu5072 at 72.964 kb on - strand, within Atu5072 at 72.973 kb on + strand at 72.981 kb on - strand at 72.981 kb on - strand at 72.983 kb on + strand at 73.014 kb on + strand at 73.029 kb on - strand at 73.123 kb on - strand at 73.129 kb on - strand at 73.222 kb on + strand, within Atu5073 at 73.222 kb on - strand, within Atu5073 at 73.224 kb on - strand, within Atu5073 at 73.312 kb on + strand, within Atu5073 at 73.320 kb on - strand, within Atu5073 at 73.396 kb on + strand, within Atu5073 at 73.396 kb on + strand, within Atu5073 at 73.478 kb on - strand, within Atu5073 at 73.484 kb on + strand, within Atu5073 at 73.526 kb on + strand, within Atu5073 at 73.532 kb on + strand, within Atu5073 at 73.532 kb on + strand, within Atu5073 at 73.542 kb on - strand, within Atu5073 at 73.585 kb on + strand, within Atu5073 at 73.616 kb on + strand, within Atu5073 at 73.649 kb on - strand, within Atu5073 at 73.649 kb on - strand, within Atu5073 at 73.713 kb on + strand, within Atu5073 at 73.784 kb on - strand, within Atu5073 at 73.805 kb on - strand, within Atu5073 at 73.822 kb on + strand, within Atu5073 at 73.827 kb on + strand, within Atu5073 at 73.832 kb on + strand, within Atu5073 at 73.893 kb on + strand, within Atu5073 at 73.911 kb on + strand, within Atu5073 at 73.950 kb on + strand, within Atu5073 at 73.958 kb on - strand, within Atu5073 at 73.958 kb on - strand, within Atu5073 at 73.958 kb on - strand, within Atu5073 at 73.965 kb on + strand at 74.054 kb on + strand at 74.088 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nicotinamide (N) remove 70,908 - Atu5071 0.58 +0.0 70,986 - Atu5071 0.63 -0.0 70,988 + Atu5071 0.63 +1.3 71,040 + Atu5071 0.66 +1.0 71,077 - Atu5071 0.69 -0.2 71,113 + Atu5071 0.71 -0.1 71,169 - Atu5071 0.75 +0.5 71,257 + Atu5071 0.81 -0.2 71,267 + Atu5071 0.81 +0.3 71,267 + Atu5071 0.81 +0.7 71,286 + Atu5071 0.83 -0.1 71,323 - Atu5071 0.85 +0.3 71,353 - Atu5071 0.87 -0.2 71,353 - Atu5071 0.87 -0.5 71,395 + Atu5071 0.90 -0.8 71,411 + +0.5 71,473 + -0.7 71,473 + +1.0 71,481 - +0.8 71,481 - -1.0 71,481 - +0.8 71,522 - -0.2 71,552 + +1.0 71,553 + +0.8 71,561 + +0.3 71,635 - -1.0 71,668 - Atu8046 0.27 +0.2 71,681 - Atu8046 0.35 +0.9 71,694 + Atu8046 0.44 +2.6 71,706 - Atu8046 0.52 +0.2 71,913 - +1.2 71,963 + +3.4 71,981 - +0.3 72,014 - +1.1 72,014 - -0.2 72,018 - +1.4 72,041 - Atu5072 0.12 -1.1 72,151 + Atu5072 0.21 -0.2 72,170 + Atu5072 0.23 +0.1 72,227 + Atu5072 0.27 +0.2 72,270 - Atu5072 0.31 -0.5 72,291 - Atu5072 0.33 +0.4 72,291 - Atu5072 0.33 +1.8 72,292 + Atu5072 0.33 +4.2 72,292 + Atu5072 0.33 +0.0 72,296 - Atu5072 0.33 -0.3 72,318 - Atu5072 0.35 -0.1 72,387 - Atu5072 0.41 +0.3 72,438 + Atu5072 0.45 +1.0 72,482 + Atu5072 0.49 +0.5 72,490 - Atu5072 0.49 +0.2 72,490 - Atu5072 0.49 -0.7 72,507 - Atu5072 0.51 -1.2 72,686 - Atu5072 0.66 +1.8 72,783 + Atu5072 0.74 +1.4 72,837 - Atu5072 0.79 +2.6 72,899 + Atu5072 0.84 -0.1 72,904 + Atu5072 0.84 +0.4 72,912 - Atu5072 0.85 +0.8 72,912 - Atu5072 0.85 +1.7 72,964 - Atu5072 0.89 -0.6 72,973 + +0.8 72,981 - +0.1 72,981 - +1.5 72,983 + +0.7 73,014 + +1.4 73,029 - -0.9 73,123 - +0.2 73,129 - +0.4 73,222 + Atu5073 0.13 +2.2 73,222 - Atu5073 0.13 +0.5 73,224 - Atu5073 0.13 +1.7 73,312 + Atu5073 0.22 +1.3 73,320 - Atu5073 0.23 -0.3 73,396 + Atu5073 0.31 +2.2 73,396 + Atu5073 0.31 +2.2 73,478 - Atu5073 0.40 -1.1 73,484 + Atu5073 0.40 +1.4 73,526 + Atu5073 0.45 +0.1 73,532 + Atu5073 0.45 +0.3 73,532 + Atu5073 0.45 -0.9 73,542 - Atu5073 0.46 +1.8 73,585 + Atu5073 0.51 +2.2 73,616 + Atu5073 0.54 +0.5 73,649 - Atu5073 0.57 +1.0 73,649 - Atu5073 0.57 -1.0 73,713 + Atu5073 0.64 -0.4 73,784 - Atu5073 0.71 +0.9 73,805 - Atu5073 0.73 -0.6 73,822 + Atu5073 0.75 -0.3 73,827 + Atu5073 0.76 -0.4 73,832 + Atu5073 0.76 +1.8 73,893 + Atu5073 0.83 +0.3 73,911 + Atu5073 0.84 -0.2 73,950 + Atu5073 0.89 +0.2 73,958 - Atu5073 0.89 +0.2 73,958 - Atu5073 0.89 +0.3 73,958 - Atu5073 0.89 +2.4 73,965 + +0.9 74,054 + +1.2 74,088 + +0.7
Or see this region's nucleotide sequence