Experiment: 2-Ethylhexanol 0.4 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4807 and PP_4808 are separated by 16 nucleotides PP_4808 and PP_4809 overlap by 41 nucleotides PP_4809 and PP_4810 are separated by 43 nucleotides
PP_4807: PP_4807 - transpeptidase (penicillin-binding protein 2), at 5,470,015 to 5,471,904
_4807
PP_4808: PP_4808 - 23S rRNA m3psi1915 methyltransferase, at 5,471,921 to 5,472,439
_4808
PP_4809: PP_4809 - Ribosomal silencing factor RsfS, at 5,472,399 to 5,472,818
_4809
PP_4810: PP_4810 - Probable nicotinate-nucleotide adenylyltransferase, at 5,472,862 to 5,473,554
_4810
Position (kb) 5471
5472
5473 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 5471.915 kb on - strand at 5471.915 kb on - strand at 5471.932 kb on - strand at 5471.965 kb on - strand at 5471.991 kb on - strand, within PP_4808 at 5472.322 kb on - strand, within PP_4808 at 5472.322 kb on - strand, within PP_4808 at 5472.401 kb on - strand at 5472.401 kb on - strand at 5472.697 kb on + strand, within PP_4809 at 5472.698 kb on - strand, within PP_4809 at 5472.698 kb on - strand, within PP_4809 at 5472.821 kb on - strand at 5472.821 kb on - strand at 5472.821 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Ethylhexanol 0.4 vol% remove 5,471,915 - +1.0 5,471,915 - +0.9 5,471,932 - -0.2 5,471,965 - -0.0 5,471,991 - PP_4808 0.13 -0.8 5,472,322 - PP_4808 0.77 -2.0 5,472,322 - PP_4808 0.77 -0.1 5,472,401 - +0.2 5,472,401 - +0.0 5,472,697 + PP_4809 0.71 -0.7 5,472,698 - PP_4809 0.71 +4.0 5,472,698 - PP_4809 0.71 +1.2 5,472,821 - -1.3 5,472,821 - -1.1 5,472,821 - +0.8
Or see this region's nucleotide sequence