Strain Fitness in Pseudomonas putida KT2440 around PP_0322

Experiment: 2-Ethylhexanol 0.4 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0321 and PP_0322 are separated by 325 nucleotidesPP_0322 and PP_0323 are separated by 15 nucleotides PP_0321: PP_0321 - low specificity L-threonine aldolase, at 385,495 to 386,535 _0321 PP_0322: PP_0322 - serine hydroxymethyltransferase, at 386,861 to 388,114 _0322 PP_0323: PP_0323 - sarcosine oxidase subunit beta, at 388,130 to 389,380 _0323 Position (kb) 386 387 388 389Strain fitness (log2 ratio) -2 -1 0 1 2at 386.002 kb on - strand, within PP_0321at 386.002 kb on - strand, within PP_0321at 386.136 kb on - strand, within PP_0321at 386.143 kb on - strand, within PP_0321at 386.300 kb on + strand, within PP_0321at 386.300 kb on + strand, within PP_0321at 386.397 kb on - strand, within PP_0321at 386.432 kb on + strandat 386.498 kb on + strandat 386.499 kb on - strandat 386.499 kb on - strandat 386.571 kb on - strandat 386.595 kb on - strandat 386.597 kb on - strandat 386.597 kb on - strandat 386.624 kb on + strandat 386.625 kb on - strandat 386.689 kb on - strandat 386.745 kb on - strandat 386.745 kb on - strandat 386.745 kb on - strandat 386.745 kb on - strandat 386.797 kb on + strandat 386.797 kb on + strandat 386.798 kb on - strandat 386.856 kb on - strandat 386.856 kb on - strandat 386.856 kb on - strandat 386.972 kb on + strandat 386.973 kb on - strandat 386.973 kb on - strandat 387.023 kb on + strand, within PP_0322at 387.024 kb on - strand, within PP_0322at 387.024 kb on - strand, within PP_0322at 387.051 kb on - strand, within PP_0322at 387.051 kb on - strand, within PP_0322at 387.053 kb on + strand, within PP_0322at 387.053 kb on + strand, within PP_0322at 387.053 kb on + strand, within PP_0322at 387.053 kb on + strand, within PP_0322at 387.053 kb on + strand, within PP_0322at 387.072 kb on + strand, within PP_0322at 387.072 kb on + strand, within PP_0322at 387.072 kb on + strand, within PP_0322at 387.073 kb on - strand, within PP_0322at 387.073 kb on - strand, within PP_0322at 387.081 kb on + strand, within PP_0322at 387.082 kb on - strand, within PP_0322at 387.082 kb on - strand, within PP_0322at 387.082 kb on - strand, within PP_0322at 387.129 kb on - strand, within PP_0322at 387.176 kb on + strand, within PP_0322at 387.278 kb on + strand, within PP_0322at 387.279 kb on - strand, within PP_0322at 387.392 kb on + strand, within PP_0322at 387.393 kb on - strand, within PP_0322at 387.541 kb on + strand, within PP_0322at 387.542 kb on - strand, within PP_0322at 387.883 kb on + strand, within PP_0322at 387.950 kb on - strand, within PP_0322at 388.107 kb on - strandat 388.274 kb on + strand, within PP_0323at 388.275 kb on - strand, within PP_0323at 388.277 kb on + strand, within PP_0323at 388.277 kb on + strand, within PP_0323at 388.328 kb on + strand, within PP_0323at 388.329 kb on - strandat 388.329 kb on - strand, within PP_0323at 388.355 kb on - strand, within PP_0323at 388.379 kb on + strand, within PP_0323at 388.406 kb on + strand, within PP_0323at 388.406 kb on + strand, within PP_0323at 388.407 kb on - strand, within PP_0323at 388.454 kb on + strand, within PP_0323at 388.454 kb on + strand, within PP_0323at 388.455 kb on - strand, within PP_0323at 388.455 kb on - strand, within PP_0323at 388.455 kb on - strand, within PP_0323at 388.455 kb on - strand, within PP_0323at 388.484 kb on + strand, within PP_0323at 388.484 kb on + strand, within PP_0323at 388.484 kb on + strand, within PP_0323at 388.485 kb on - strand, within PP_0323at 388.485 kb on - strand, within PP_0323at 388.605 kb on - strand, within PP_0323at 388.654 kb on - strand, within PP_0323at 388.976 kb on + strand, within PP_0323at 388.976 kb on + strand, within PP_0323

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Ethylhexanol 0.4 vol%
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386,002 - PP_0321 0.49 +0.7
386,002 - PP_0321 0.49 -0.3
386,136 - PP_0321 0.62 +0.1
386,143 - PP_0321 0.62 +0.3
386,300 + PP_0321 0.77 +0.4
386,300 + PP_0321 0.77 -0.4
386,397 - PP_0321 0.87 -0.3
386,432 + +0.6
386,498 + +0.2
386,499 - -1.0
386,499 - +0.7
386,571 - +0.2
386,595 - +0.2
386,597 - -1.2
386,597 - -0.1
386,624 + +1.2
386,625 - -0.2
386,689 - -0.3
386,745 - +0.0
386,745 - -0.8
386,745 - -0.1
386,745 - -0.3
386,797 + +0.9
386,797 + -1.1
386,798 - +0.9
386,856 - +0.8
386,856 - -1.3
386,856 - -1.1
386,972 + -0.5
386,973 - -1.8
386,973 - -0.5
387,023 + PP_0322 0.13 +1.1
387,024 - PP_0322 0.13 -0.9
387,024 - PP_0322 0.13 -0.1
387,051 - PP_0322 0.15 -0.6
387,051 - PP_0322 0.15 +0.0
387,053 + PP_0322 0.15 -0.6
387,053 + PP_0322 0.15 -0.1
387,053 + PP_0322 0.15 +1.1
387,053 + PP_0322 0.15 +1.2
387,053 + PP_0322 0.15 -0.2
387,072 + PP_0322 0.17 +0.8
387,072 + PP_0322 0.17 -0.2
387,072 + PP_0322 0.17 -0.7
387,073 - PP_0322 0.17 +0.3
387,073 - PP_0322 0.17 -0.5
387,081 + PP_0322 0.18 -2.6
387,082 - PP_0322 0.18 +0.1
387,082 - PP_0322 0.18 +0.4
387,082 - PP_0322 0.18 -1.7
387,129 - PP_0322 0.21 -0.1
387,176 + PP_0322 0.25 +0.5
387,278 + PP_0322 0.33 +0.3
387,279 - PP_0322 0.33 +0.9
387,392 + PP_0322 0.42 +0.1
387,393 - PP_0322 0.42 -0.4
387,541 + PP_0322 0.54 +0.2
387,542 - PP_0322 0.54 +0.5
387,883 + PP_0322 0.81 +0.0
387,950 - PP_0322 0.87 +0.5
388,107 - +0.2
388,274 + PP_0323 0.12 -0.5
388,275 - PP_0323 0.12 -0.0
388,277 + PP_0323 0.12 -0.1
388,277 + PP_0323 0.12 -0.7
388,328 + PP_0323 0.16 -0.7
388,329 - -0.9
388,329 - PP_0323 0.16 -0.4
388,355 - PP_0323 0.18 -2.4
388,379 + PP_0323 0.20 +0.1
388,406 + PP_0323 0.22 +0.1
388,406 + PP_0323 0.22 -0.1
388,407 - PP_0323 0.22 -0.1
388,454 + PP_0323 0.26 +0.5
388,454 + PP_0323 0.26 +0.0
388,455 - PP_0323 0.26 -0.8
388,455 - PP_0323 0.26 -0.6
388,455 - PP_0323 0.26 -1.1
388,455 - PP_0323 0.26 -0.0
388,484 + PP_0323 0.28 -0.2
388,484 + PP_0323 0.28 -0.0
388,484 + PP_0323 0.28 -0.1
388,485 - PP_0323 0.28 -0.7
388,485 - PP_0323 0.28 -0.7
388,605 - PP_0323 0.38 -1.7
388,654 - PP_0323 0.42 -0.3
388,976 + PP_0323 0.68 +2.7
388,976 + PP_0323 0.68 +0.8

Or see this region's nucleotide sequence