Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4809 and GFF4810 overlap by 4 nucleotides GFF4810 and GFF4811 overlap by 19 nucleotides GFF4811 and GFF4812 are separated by 10 nucleotides
GFF4809 - CRISPR-associated protein Cas1, at 108,503 to 109,423
GFF4809
GFF4810 - CRISPR-associated protein, Cse3 family, at 109,420 to 110,070
GFF4810
GFF4811 - CRISPR-associated protein, Cas5e family, at 110,052 to 110,798
GFF4811
GFF4812 - CRISPR-associated protein, Cse4 family, at 110,809 to 111,867
GFF4812
Position (kb) 110
111 Strain fitness (log2 ratio) -2
-1
0
1 at 109.271 kb on - strand, within GFF4809 at 109.271 kb on - strand, within GFF4809 at 109.295 kb on - strand, within GFF4809 at 109.359 kb on + strand at 109.383 kb on - strand at 109.477 kb on - strand at 109.541 kb on + strand, within GFF4810 at 109.723 kb on - strand, within GFF4810 at 110.269 kb on + strand, within GFF4811 at 110.270 kb on - strand, within GFF4811 at 110.356 kb on + strand, within GFF4811 at 110.379 kb on + strand, within GFF4811 at 110.380 kb on - strand, within GFF4811 at 110.458 kb on + strand, within GFF4811 at 110.537 kb on + strand, within GFF4811 at 110.542 kb on - strand, within GFF4811 at 110.542 kb on - strand, within GFF4811 at 110.679 kb on + strand, within GFF4811 at 110.679 kb on + strand, within GFF4811 at 110.679 kb on + strand, within GFF4811 at 110.680 kb on - strand, within GFF4811 at 110.680 kb on - strand, within GFF4811 at 110.710 kb on - strand, within GFF4811 at 110.902 kb on - strand at 110.913 kb on + strand at 110.913 kb on + strand at 110.914 kb on - strand at 110.925 kb on + strand, within GFF4812 at 110.925 kb on + strand, within GFF4812 at 110.925 kb on + strand, within GFF4812 at 110.926 kb on - strand, within GFF4812 at 111.039 kb on - strand, within GFF4812 at 111.179 kb on + strand, within GFF4812 at 111.180 kb on - strand, within GFF4812 at 111.180 kb on - strand, within GFF4812 at 111.180 kb on - strand, within GFF4812 at 111.413 kb on - strand, within GFF4812 at 111.555 kb on + strand, within GFF4812 at 111.600 kb on - strand, within GFF4812 at 111.684 kb on + strand, within GFF4812 at 111.695 kb on - strand, within GFF4812 at 111.710 kb on - strand, within GFF4812 at 111.720 kb on + strand, within GFF4812 at 111.721 kb on - strand, within GFF4812
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 109,271 - GFF4809 0.83 -0.1 109,271 - GFF4809 0.83 +0.4 109,295 - GFF4809 0.86 -0.6 109,359 + -0.4 109,383 - +0.2 109,477 - -0.2 109,541 + GFF4810 0.19 -1.3 109,723 - GFF4810 0.47 +0.7 110,269 + GFF4811 0.29 -0.2 110,270 - GFF4811 0.29 +0.4 110,356 + GFF4811 0.41 +1.0 110,379 + GFF4811 0.44 -0.1 110,380 - GFF4811 0.44 -1.1 110,458 + GFF4811 0.54 -0.4 110,537 + GFF4811 0.65 +0.1 110,542 - GFF4811 0.66 -2.0 110,542 - GFF4811 0.66 -1.1 110,679 + GFF4811 0.84 -0.9 110,679 + GFF4811 0.84 -1.2 110,679 + GFF4811 0.84 -0.0 110,680 - GFF4811 0.84 +0.9 110,680 - GFF4811 0.84 +0.9 110,710 - GFF4811 0.88 -0.1 110,902 - -1.4 110,913 + +0.3 110,913 + +1.0 110,914 - +0.8 110,925 + GFF4812 0.11 +0.0 110,925 + GFF4812 0.11 +0.1 110,925 + GFF4812 0.11 +0.3 110,926 - GFF4812 0.11 -0.3 111,039 - GFF4812 0.22 -0.1 111,179 + GFF4812 0.35 -0.7 111,180 - GFF4812 0.35 -0.4 111,180 - GFF4812 0.35 -0.4 111,180 - GFF4812 0.35 -0.4 111,413 - GFF4812 0.57 +0.2 111,555 + GFF4812 0.70 -0.1 111,600 - GFF4812 0.75 +0.5 111,684 + GFF4812 0.83 -0.3 111,695 - GFF4812 0.84 +0.3 111,710 - GFF4812 0.85 -0.7 111,720 + GFF4812 0.86 +0.2 111,721 - GFF4812 0.86 +0.5
Or see this region's nucleotide sequence