Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4158

Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4156 and GFF4157 are separated by 315 nucleotidesGFF4157 and GFF4158 are separated by 145 nucleotidesGFF4158 and GFF4159 are separated by 236 nucleotidesGFF4159 and GFF4160 are separated by 4 nucleotides GFF4156 - T1SS secreted agglutinin RTX, at 38,443 to 49,917 GFF4156 GFF4157 - hypothetical protein, at 50,233 to 50,385 GFF4157 GFF4158 - tmRNA-binding protein SmpB, at 50,531 to 51,013 GFF4158 GFF4159 - Putative oligoketide cyclase/lipid transport protein, similarity with yeast ubiquinone-binding protein YOL008W, at 51,250 to 51,639 GFF4159 GFF4160 - UPF0125 protein yfjF, at 51,644 to 51,919 GFF4160 Position (kb) 50 51 52Strain fitness (log2 ratio) -3 -2 -1 0 1at 49.612 kb on - strandat 49.667 kb on - strandat 49.693 kb on + strandat 49.918 kb on - strandat 49.918 kb on - strandat 50.123 kb on - strandat 50.424 kb on + strandat 50.458 kb on + strandat 50.851 kb on - strand, within GFF4158at 50.943 kb on + strand, within GFF4158at 51.035 kb on - strandat 51.085 kb on - strandat 51.089 kb on + strandat 51.090 kb on - strandat 51.090 kb on - strandat 51.119 kb on - strandat 51.161 kb on - strandat 51.171 kb on - strandat 51.260 kb on + strandat 51.680 kb on + strand, within GFF4160at 51.781 kb on + strand, within GFF4160at 51.786 kb on + strand, within GFF4160at 51.787 kb on - strand, within GFF4160at 51.794 kb on + strand, within GFF4160at 51.882 kb on + strand, within GFF4160at 51.883 kb on - strand, within GFF4160at 51.883 kb on - strand, within GFF4160at 51.913 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI
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49,612 - +0.2
49,667 - -1.2
49,693 + +0.1
49,918 - -0.3
49,918 - -0.7
50,123 - -1.4
50,424 + +0.3
50,458 + -1.7
50,851 - GFF4158 0.66 -2.8
50,943 + GFF4158 0.85 -1.6
51,035 - -1.8
51,085 - -0.3
51,089 + -2.4
51,090 - +0.2
51,090 - +0.1
51,119 - -0.3
51,161 - +0.0
51,171 - -1.2
51,260 + -2.4
51,680 + GFF4160 0.13 -0.5
51,781 + GFF4160 0.50 +0.2
51,786 + GFF4160 0.51 -0.1
51,787 - GFF4160 0.52 -1.0
51,794 + GFF4160 0.54 -2.0
51,882 + GFF4160 0.86 +0.8
51,883 - GFF4160 0.87 -1.4
51,883 - GFF4160 0.87 +0.6
51,913 + -0.8

Or see this region's nucleotide sequence