Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4040 and GFF4041 are separated by 131 nucleotides GFF4041 and GFF4042 are separated by 77 nucleotides GFF4042 and GFF4043 are separated by 55 nucleotides
GFF4040 - Putative drug efflux protein, at 2,322 to 3,497
GFF4040
GFF4041 - FIG00613320: hypothetical protein, at 3,629 to 4,429
GFF4041
GFF4042 - SAM-dependent methyltransferase YafE (UbiE paralog), at 4,507 to 5,277
GFF4042
GFF4043 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 5,333 to 6,553
GFF4043
Position (kb) 3
4
5 Strain fitness (log2 ratio) -2
-1
0
1
2 at 2.711 kb on + strand, within GFF4040 at 2.712 kb on - strand, within GFF4040 at 2.712 kb on - strand, within GFF4040 at 2.735 kb on - strand, within GFF4040 at 3.062 kb on - strand, within GFF4040 at 3.192 kb on + strand, within GFF4040 at 3.262 kb on - strand, within GFF4040 at 3.262 kb on - strand, within GFF4040 at 3.302 kb on - strand, within GFF4040 at 3.509 kb on - strand at 3.644 kb on + strand at 3.780 kb on + strand, within GFF4041 at 3.899 kb on - strand, within GFF4041 at 3.899 kb on - strand, within GFF4041 at 3.934 kb on - strand, within GFF4041 at 3.986 kb on + strand, within GFF4041 at 4.051 kb on - strand, within GFF4041 at 4.053 kb on - strand, within GFF4041 at 4.086 kb on + strand, within GFF4041 at 4.108 kb on + strand, within GFF4041 at 4.125 kb on + strand, within GFF4041 at 4.126 kb on - strand, within GFF4041 at 4.140 kb on + strand, within GFF4041 at 4.222 kb on + strand, within GFF4041 at 4.223 kb on - strand, within GFF4041 at 4.338 kb on + strand, within GFF4041 at 4.479 kb on - strand at 4.571 kb on + strand at 4.572 kb on - strand at 4.572 kb on - strand at 4.779 kb on - strand, within GFF4042 at 4.782 kb on + strand, within GFF4042 at 4.785 kb on + strand, within GFF4042 at 4.805 kb on - strand, within GFF4042 at 5.025 kb on + strand, within GFF4042 at 5.029 kb on + strand, within GFF4042 at 5.029 kb on + strand, within GFF4042 at 5.030 kb on - strand, within GFF4042 at 5.139 kb on + strand, within GFF4042 at 5.247 kb on + strand at 5.247 kb on + strand at 5.276 kb on - strand at 5.332 kb on - strand at 5.350 kb on + strand at 5.372 kb on - strand at 5.391 kb on - strand at 5.401 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 2,711 + GFF4040 0.33 -1.6 2,712 - GFF4040 0.33 +0.8 2,712 - GFF4040 0.33 -0.9 2,735 - GFF4040 0.35 +1.1 3,062 - GFF4040 0.63 +0.2 3,192 + GFF4040 0.74 -2.4 3,262 - GFF4040 0.80 +0.4 3,262 - GFF4040 0.80 -0.6 3,302 - GFF4040 0.83 +0.1 3,509 - -2.0 3,644 + +0.8 3,780 + GFF4041 0.19 -0.3 3,899 - GFF4041 0.34 +0.4 3,899 - GFF4041 0.34 +0.4 3,934 - GFF4041 0.38 +0.6 3,986 + GFF4041 0.45 +1.5 4,051 - GFF4041 0.53 -0.7 4,053 - GFF4041 0.53 +0.7 4,086 + GFF4041 0.57 +0.6 4,108 + GFF4041 0.60 +0.5 4,125 + GFF4041 0.62 +1.5 4,126 - GFF4041 0.62 +1.3 4,140 + GFF4041 0.64 +0.4 4,222 + GFF4041 0.74 +0.6 4,223 - GFF4041 0.74 +0.8 4,338 + GFF4041 0.89 +0.9 4,479 - +0.4 4,571 + +0.0 4,572 - -1.0 4,572 - -0.1 4,779 - GFF4042 0.35 +0.3 4,782 + GFF4042 0.36 -0.5 4,785 + GFF4042 0.36 +0.1 4,805 - GFF4042 0.39 -0.9 5,025 + GFF4042 0.67 -0.2 5,029 + GFF4042 0.68 -0.3 5,029 + GFF4042 0.68 +0.1 5,030 - GFF4042 0.68 -0.7 5,139 + GFF4042 0.82 -0.0 5,247 + -0.3 5,247 + +0.4 5,276 - +0.1 5,332 - -0.2 5,350 + +1.9 5,372 - +0.3 5,391 - -0.6 5,401 + -0.2
Or see this region's nucleotide sequence