Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3974 and GFF3975 are separated by 79 nucleotides GFF3975 and GFF3976 are separated by 319 nucleotides GFF3976 and GFF3977 are separated by 44 nucleotides GFF3977 and GFF3978 are separated by 30 nucleotides
GFF3974 - AMP nucleosidase (EC 3.2.2.4), at 252,092 to 253,546
GFF3974
GFF3975 - hypothetical protein, at 253,626 to 253,748
GFF3975
GFF3976 - FIG01045899: hypothetical protein, at 254,068 to 254,241
GFF3976
GFF3977 - hypothetical protein, at 254,286 to 254,420
GFF3977
GFF3978 - Putative periplasmic protein, at 254,451 to 256,181
GFF3978
Position (kb) 253
254 Strain fitness (log2 ratio) -1
0
1
2
3 at 252.774 kb on - strand, within GFF3974 at 252.923 kb on - strand, within GFF3974 at 253.061 kb on + strand, within GFF3974 at 253.061 kb on + strand, within GFF3974 at 253.131 kb on + strand, within GFF3974 at 253.300 kb on + strand, within GFF3974 at 253.349 kb on + strand, within GFF3974 at 253.383 kb on + strand, within GFF3974 at 253.592 kb on - strand at 253.596 kb on - strand at 253.603 kb on - strand at 253.716 kb on + strand, within GFF3975 at 253.797 kb on + strand at 253.834 kb on - strand at 253.886 kb on - strand at 253.887 kb on + strand at 253.887 kb on + strand at 253.887 kb on + strand at 253.888 kb on - strand at 254.066 kb on - strand at 254.071 kb on + strand at 254.071 kb on + strand at 254.487 kb on - strand at 254.489 kb on - strand at 254.636 kb on + strand, within GFF3978
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 252,774 - GFF3974 0.47 +0.4 252,923 - GFF3974 0.57 +0.4 253,061 + GFF3974 0.67 -1.2 253,061 + GFF3974 0.67 -0.3 253,131 + GFF3974 0.71 +0.4 253,300 + GFF3974 0.83 -0.3 253,349 + GFF3974 0.86 +0.1 253,383 + GFF3974 0.89 +0.2 253,592 - +3.3 253,596 - +1.3 253,603 - +0.2 253,716 + GFF3975 0.73 -0.8 253,797 + +0.0 253,834 - +0.4 253,886 - -1.1 253,887 + +0.3 253,887 + +0.5 253,887 + +0.3 253,888 - +0.5 254,066 - +0.3 254,071 + +0.2 254,071 + -0.1 254,487 - +0.4 254,489 - -0.7 254,636 + GFF3978 0.11 -0.6
Or see this region's nucleotide sequence