Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3887 and GFF3888 overlap by 1 nucleotides GFF3888 and GFF3889 are separated by 41 nucleotides GFF3889 and GFF3890 are separated by 3 nucleotides
GFF3887 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 166,273 to 167,358
GFF3887
GFF3888 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 167,358 to 168,257
GFF3888
GFF3889 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 168,299 to 169,183
GFF3889
GFF3890 - dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13), at 169,187 to 169,735
GFF3890
Position (kb) 167
168
169 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 166.585 kb on + strand, within GFF3887 at 166.612 kb on + strand, within GFF3887 at 166.764 kb on + strand, within GFF3887 at 166.825 kb on + strand, within GFF3887 at 166.857 kb on + strand, within GFF3887 at 166.860 kb on + strand, within GFF3887 at 166.864 kb on + strand, within GFF3887 at 166.864 kb on + strand, within GFF3887 at 166.973 kb on + strand, within GFF3887 at 166.975 kb on + strand, within GFF3887 at 166.976 kb on - strand, within GFF3887 at 166.976 kb on - strand, within GFF3887 at 167.004 kb on + strand, within GFF3887 at 167.082 kb on - strand, within GFF3887 at 167.086 kb on - strand, within GFF3887 at 167.149 kb on + strand, within GFF3887 at 167.150 kb on - strand, within GFF3887 at 167.150 kb on - strand, within GFF3887 at 167.341 kb on + strand at 167.368 kb on + strand at 167.510 kb on + strand, within GFF3888 at 167.557 kb on + strand, within GFF3888 at 167.673 kb on + strand, within GFF3888 at 167.828 kb on + strand, within GFF3888 at 167.828 kb on + strand, within GFF3888 at 168.043 kb on + strand, within GFF3888 at 168.095 kb on + strand, within GFF3888 at 168.264 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 166,585 + GFF3887 0.29 -0.0 166,612 + GFF3887 0.31 -0.4 166,764 + GFF3887 0.45 -1.1 166,825 + GFF3887 0.51 -1.3 166,857 + GFF3887 0.54 -0.1 166,860 + GFF3887 0.54 +1.7 166,864 + GFF3887 0.54 -0.4 166,864 + GFF3887 0.54 -0.5 166,973 + GFF3887 0.64 -2.0 166,975 + GFF3887 0.65 +0.3 166,976 - GFF3887 0.65 +4.8 166,976 - GFF3887 0.65 +5.7 167,004 + GFF3887 0.67 -1.4 167,082 - GFF3887 0.74 +5.0 167,086 - GFF3887 0.75 +4.1 167,149 + GFF3887 0.81 -1.3 167,150 - GFF3887 0.81 +4.2 167,150 - GFF3887 0.81 +4.1 167,341 + +0.0 167,368 + +4.6 167,510 + GFF3888 0.17 +6.2 167,557 + GFF3888 0.22 +2.9 167,673 + GFF3888 0.35 +4.9 167,828 + GFF3888 0.52 +5.3 167,828 + GFF3888 0.52 +4.7 168,043 + GFF3888 0.76 +5.3 168,095 + GFF3888 0.82 +5.7 168,264 + -0.3
Or see this region's nucleotide sequence