Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3752 and GFF3753 are separated by 151 nucleotides GFF3753 and GFF3754 are separated by 27 nucleotides GFF3754 and GFF3755 are separated by 390 nucleotides GFF3755 and GFF3756 are separated by 3 nucleotides
GFF3752 - ATP-dependent RNA helicase YejH, at 18,543 to 20,303
GFF3752
GFF3753 - Ribosomal small subunit pseudouridine synthase A (EC 4.2.1.70), at 20,455 to 21,150
GFF3753
GFF3754 - MFS family multidrug transport protein, bicyclomycin resistance protein, at 21,178 to 22,368
GFF3754
GFF3755 - FIG00896318: hypothetical protein, at 22,759 to 23,103
GFF3755
GFF3756 - Putative ABC transporter ATP-binding protein, at 23,107 to 24,696
GFF3756
Position (kb)
21
22
23 Strain fitness (log2 ratio)
-1
0
1 at 20.366 kb on + strand at 20.367 kb on - strand at 20.367 kb on - strand at 20.436 kb on + strand at 20.639 kb on - strand, within GFF3753 at 20.639 kb on - strand, within GFF3753 at 20.649 kb on + strand, within GFF3753 at 20.731 kb on + strand, within GFF3753 at 20.818 kb on - strand, within GFF3753 at 20.848 kb on + strand, within GFF3753 at 20.849 kb on - strand, within GFF3753 at 21.079 kb on - strand, within GFF3753 at 21.152 kb on + strand at 21.152 kb on + strand at 21.152 kb on + strand at 21.240 kb on - strand at 21.263 kb on - strand at 21.263 kb on - strand at 21.337 kb on + strand, within GFF3754 at 21.340 kb on - strand, within GFF3754 at 21.399 kb on + strand, within GFF3754 at 21.428 kb on + strand, within GFF3754 at 21.557 kb on - strand, within GFF3754 at 21.654 kb on - strand, within GFF3754 at 21.670 kb on + strand, within GFF3754 at 21.672 kb on + strand, within GFF3754 at 21.672 kb on + strand, within GFF3754 at 21.672 kb on + strand, within GFF3754 at 21.673 kb on - strand, within GFF3754 at 21.786 kb on - strand, within GFF3754 at 21.786 kb on - strand, within GFF3754 at 21.786 kb on - strand, within GFF3754 at 21.829 kb on + strand, within GFF3754 at 21.895 kb on + strand, within GFF3754 at 22.471 kb on - strand at 22.471 kb on - strand at 22.471 kb on - strand at 22.533 kb on + strand at 22.555 kb on + strand at 22.590 kb on + strand at 22.624 kb on - strand at 22.769 kb on + strand at 22.890 kb on + strand, within GFF3755 at 22.936 kb on - strand, within GFF3755 at 23.091 kb on + strand at 23.091 kb on + strand at 23.101 kb on + strand at 23.134 kb on - strand at 23.136 kb on - strand at 23.234 kb on - strand at 23.251 kb on - strand at 23.261 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 20,366 + -0.4 20,367 - +0.2 20,367 - +0.6 20,436 + +0.3 20,639 - GFF3753 0.26 +0.5 20,639 - GFF3753 0.26 +0.1 20,649 + GFF3753 0.28 -0.3 20,731 + GFF3753 0.40 +0.3 20,818 - GFF3753 0.52 -0.0 20,848 + GFF3753 0.56 +1.5 20,849 - GFF3753 0.57 -0.1 21,079 - GFF3753 0.90 -1.5 21,152 + +0.6 21,152 + -0.8 21,152 + +0.3 21,240 - +0.9 21,263 - -0.1 21,263 - +0.9 21,337 + GFF3754 0.13 +0.2 21,340 - GFF3754 0.14 +0.4 21,399 + GFF3754 0.19 +0.6 21,428 + GFF3754 0.21 +0.2 21,557 - GFF3754 0.32 -0.3 21,654 - GFF3754 0.40 -0.0 21,670 + GFF3754 0.41 -0.8 21,672 + GFF3754 0.41 +0.8 21,672 + GFF3754 0.41 +0.8 21,672 + GFF3754 0.41 +0.9 21,673 - GFF3754 0.42 +1.6 21,786 - GFF3754 0.51 +0.1 21,786 - GFF3754 0.51 +0.5 21,786 - GFF3754 0.51 +0.7 21,829 + GFF3754 0.55 +0.1 21,895 + GFF3754 0.60 +0.0 22,471 - -0.1 22,471 - -0.1 22,471 - +0.3 22,533 + +0.1 22,555 + +0.0 22,590 + +0.5 22,624 - -0.5 22,769 + +1.2 22,890 + GFF3755 0.38 +0.7 22,936 - GFF3755 0.51 -1.7 23,091 + -1.4 23,091 + -0.2 23,101 + -0.3 23,134 - +0.6 23,136 - +0.3 23,234 - +0.5 23,251 - -1.0 23,261 + +0.6
Or see this region's nucleotide sequence