Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3508 and GFF3509 are separated by 364 nucleotides GFF3509 and GFF3510 are separated by 101 nucleotides GFF3510 and GFF3511 are separated by 136 nucleotides GFF3511 and GFF3512 are separated by 137 nucleotides
GFF3508 - Catalase (EC 1.11.1.6), at 259,167 to 261,419
GFF3508
GFF3509 - Cell division activator cedA, at 261,784 to 262,026
GFF3509
GFF3510 - L-cystine uptake protein TcyP, at 262,128 to 263,519
GFF3510
GFF3511 - Membrane-bound metal-dependent hydrolase YdjM, induced during SOS response, at 263,656 to 264,255
GFF3511
GFF3512 - 2-deoxyglucose-6-phosphate hydrolase YniC, at 264,393 to 265,061
GFF3512
Position (kb) 262
263
264 Strain fitness (log2 ratio) -2
-1
0
1
2 at 261.410 kb on + strand at 261.500 kb on + strand at 261.532 kb on + strand at 261.535 kb on - strand at 261.535 kb on - strand at 261.819 kb on + strand, within GFF3509 at 261.822 kb on + strand, within GFF3509 at 261.826 kb on + strand, within GFF3509 at 261.826 kb on + strand, within GFF3509 at 261.827 kb on - strand, within GFF3509 at 261.828 kb on + strand, within GFF3509 at 261.828 kb on + strand, within GFF3509 at 261.907 kb on - strand, within GFF3509 at 261.910 kb on + strand, within GFF3509 at 261.911 kb on - strand, within GFF3509 at 261.961 kb on - strand, within GFF3509 at 262.127 kb on - strand at 262.129 kb on + strand at 262.175 kb on + strand at 262.575 kb on - strand, within GFF3510 at 262.678 kb on - strand, within GFF3510 at 262.681 kb on - strand, within GFF3510 at 262.717 kb on + strand, within GFF3510 at 262.718 kb on - strand, within GFF3510 at 262.749 kb on - strand, within GFF3510 at 262.749 kb on - strand, within GFF3510 at 262.812 kb on - strand, within GFF3510 at 262.967 kb on + strand, within GFF3510 at 263.060 kb on - strand, within GFF3510 at 263.169 kb on - strand, within GFF3510 at 263.448 kb on - strand at 263.506 kb on - strand at 263.703 kb on + strand at 263.706 kb on + strand at 263.728 kb on + strand, within GFF3511 at 263.978 kb on + strand, within GFF3511
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 261,410 + -1.4 261,500 + -1.2 261,532 + -0.3 261,535 - -1.1 261,535 - +0.6 261,819 + GFF3509 0.14 -0.1 261,822 + GFF3509 0.16 -0.1 261,826 + GFF3509 0.17 -1.0 261,826 + GFF3509 0.17 -0.9 261,827 - GFF3509 0.18 +0.6 261,828 + GFF3509 0.18 -0.8 261,828 + GFF3509 0.18 -0.6 261,907 - GFF3509 0.51 -0.3 261,910 + GFF3509 0.52 +0.6 261,911 - GFF3509 0.52 +0.8 261,961 - GFF3509 0.73 -0.4 262,127 - +0.2 262,129 + -0.1 262,175 + -0.9 262,575 - GFF3510 0.32 -1.5 262,678 - GFF3510 0.40 -0.5 262,681 - GFF3510 0.40 -0.7 262,717 + GFF3510 0.42 +0.9 262,718 - GFF3510 0.42 +0.7 262,749 - GFF3510 0.45 -0.9 262,749 - GFF3510 0.45 -0.5 262,812 - GFF3510 0.49 -1.0 262,967 + GFF3510 0.60 -1.0 263,060 - GFF3510 0.67 -0.0 263,169 - GFF3510 0.75 -1.8 263,448 - +2.7 263,506 - +0.0 263,703 + +0.8 263,706 + -0.1 263,728 + GFF3511 0.12 +0.6 263,978 + GFF3511 0.54 +0.6
Or see this region's nucleotide sequence