Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3450 and GFF3451 are separated by 254 nucleotides GFF3451 and GFF3452 are separated by 138 nucleotides GFF3452 and GFF3453 are separated by 82 nucleotides GFF3453 and GFF3454 are separated by 196 nucleotides GFF3454 and GFF3455 are separated by 16 nucleotides
GFF3450 - Transcriptional regulator, MerR family, at 210,243 to 211,091
GFF3450
GFF3451 - FIG074102: hypothetical protein, at 211,346 to 211,534
GFF3451
GFF3452 - FIG01045247: hypothetical protein, at 211,673 to 211,822
GFF3452
GFF3453 - Periplasmic chorismate mutase I precursor (EC 5.4.99.5), at 211,905 to 212,450
GFF3453
GFF3454 - Putative transport protein, at 212,647 to 213,285
GFF3454
GFF3455 - FIG01046394: hypothetical protein, at 213,302 to 213,481
GFF3455
Position (kb) 211
212
213 Strain fitness (log2 ratio) -1
0
1
2 at 211.124 kb on + strand at 211.125 kb on - strand at 211.344 kb on + strand at 212.206 kb on - strand, within GFF3453 at 212.209 kb on + strand, within GFF3453 at 212.276 kb on + strand, within GFF3453 at 212.448 kb on + strand at 212.477 kb on - strand at 212.623 kb on - strand at 212.660 kb on - strand at 212.776 kb on + strand, within GFF3454 at 212.777 kb on - strand, within GFF3454 at 212.903 kb on - strand, within GFF3454 at 212.911 kb on + strand, within GFF3454 at 212.911 kb on + strand, within GFF3454
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 211,124 + +0.3 211,125 - -1.0 211,344 + -0.0 212,206 - GFF3453 0.55 -0.7 212,209 + GFF3453 0.56 -1.5 212,276 + GFF3453 0.68 +0.7 212,448 + -0.4 212,477 - -1.4 212,623 - +0.6 212,660 - +0.4 212,776 + GFF3454 0.20 +0.6 212,777 - GFF3454 0.20 +0.2 212,903 - GFF3454 0.40 +0.6 212,911 + GFF3454 0.41 -0.3 212,911 + GFF3454 0.41 +2.2
Or see this region's nucleotide sequence