Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3647 and GFF3430 are separated by 754 nucleotides GFF3430 and GFF3431 are separated by 377 nucleotides GFF3431 and GFF3432 are separated by 131 nucleotides GFF3432 and GFF3433 are separated by 44 nucleotides
GFF3647 - tRNA-Arg-CCT, at 195,266 to 195,342
GFF3647
GFF3430 - Periplasmic lysozyme inhibitor of c-type lysozyme, at 196,097 to 196,441
GFF3430
GFF3431 - hypothetical protein, at 196,819 to 196,944
GFF3431
GFF3432 - FIG01046599: hypothetical protein, at 197,076 to 197,249
GFF3432
GFF3433 - FIG074102: hypothetical protein, at 197,294 to 197,416
GFF3433
Position (kb) 196
197 Strain fitness (log2 ratio) -1
0
1
2 at 195.681 kb on + strand at 195.723 kb on + strand at 195.970 kb on + strand at 195.970 kb on + strand at 196.026 kb on + strand at 196.132 kb on + strand, within GFF3430 at 196.132 kb on + strand, within GFF3430 at 196.172 kb on - strand, within GFF3430 at 196.209 kb on + strand, within GFF3430 at 196.305 kb on + strand, within GFF3430 at 196.346 kb on - strand, within GFF3430 at 196.351 kb on + strand, within GFF3430 at 196.351 kb on + strand, within GFF3430 at 196.442 kb on + strand at 196.493 kb on - strand at 196.521 kb on - strand at 196.586 kb on + strand at 196.732 kb on - strand at 196.835 kb on + strand, within GFF3431 at 196.835 kb on + strand, within GFF3431 at 196.974 kb on - strand at 197.331 kb on + strand, within GFF3433
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 195,681 + -0.7 195,723 + -0.1 195,970 + -0.3 195,970 + +0.6 196,026 + -0.0 196,132 + GFF3430 0.10 +0.3 196,132 + GFF3430 0.10 -1.3 196,172 - GFF3430 0.22 -0.8 196,209 + GFF3430 0.32 +2.4 196,305 + GFF3430 0.60 -1.1 196,346 - GFF3430 0.72 +0.1 196,351 + GFF3430 0.74 -0.9 196,351 + GFF3430 0.74 -0.6 196,442 + -0.1 196,493 - +1.0 196,521 - -0.5 196,586 + -0.8 196,732 - -0.3 196,835 + GFF3431 0.13 -0.3 196,835 + GFF3431 0.13 +0.3 196,974 - +0.3 197,331 + GFF3433 0.30 -0.1
Or see this region's nucleotide sequence