Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3385 and GFF3386 are separated by 323 nucleotides GFF3386 and GFF3387 are separated by 2 nucleotides GFF3387 and GFF3388 are separated by 13 nucleotides GFF3388 and GFF3389 overlap by 20 nucleotides
GFF3385 - Putative OMR family iron-siderophore receptor precursor, at 149,939 to 152,113
GFF3385
GFF3386 - YcfF/hinT protein: a purine nucleoside phosphoramidase, at 152,437 to 152,814
GFF3386
GFF3387 - YcfL protein: an outer membrane lipoprotein that is part of a salvage cluster, at 152,817 to 153,191
GFF3387
GFF3388 - Lipoprotein YcfM, part of a salvage pathway of unknown substrate, at 153,205 to 153,843
GFF3388
GFF3389 - Thiamine kinase (EC 2.7.1.89) @ Adenosylcobinamide kinase (EC 2.7.1.156), at 153,824 to 154,648
GFF3389
Position (kb)
152
153
154 Strain fitness (log2 ratio)
-2
-1
0
1 at 152.027 kb on - strand at 152.047 kb on + strand at 152.052 kb on - strand at 152.246 kb on + strand at 152.324 kb on - strand at 152.389 kb on + strand at 152.412 kb on + strand at 152.571 kb on + strand, within GFF3386 at 152.718 kb on + strand, within GFF3386 at 152.746 kb on + strand, within GFF3386 at 152.751 kb on + strand, within GFF3386 at 152.812 kb on + strand at 152.813 kb on - strand at 152.816 kb on + strand at 152.816 kb on + strand at 152.817 kb on - strand at 153.048 kb on + strand, within GFF3387 at 153.133 kb on + strand, within GFF3387 at 153.183 kb on + strand at 153.264 kb on + strand at 153.433 kb on + strand, within GFF3388 at 153.433 kb on + strand, within GFF3388 at 153.507 kb on - strand, within GFF3388 at 153.529 kb on - strand, within GFF3388 at 153.576 kb on - strand, within GFF3388 at 153.688 kb on - strand, within GFF3388 at 153.729 kb on - strand, within GFF3388 at 153.734 kb on - strand, within GFF3388 at 153.771 kb on - strand, within GFF3388 at 153.782 kb on + strand at 153.783 kb on - strand at 153.807 kb on - strand at 153.842 kb on - strand at 153.949 kb on + strand, within GFF3389 at 154.069 kb on + strand, within GFF3389 at 154.089 kb on + strand, within GFF3389 at 154.155 kb on - strand, within GFF3389 at 154.155 kb on - strand, within GFF3389 at 154.155 kb on - strand, within GFF3389
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 152,027 - +1.6 152,047 + -0.1 152,052 - +0.2 152,246 + +0.2 152,324 - +1.1 152,389 + +0.3 152,412 + +0.7 152,571 + GFF3386 0.35 -0.3 152,718 + GFF3386 0.74 -1.3 152,746 + GFF3386 0.82 -0.9 152,751 + GFF3386 0.83 -0.2 152,812 + +0.4 152,813 - +1.2 152,816 + +0.3 152,816 + -0.9 152,817 - -0.2 153,048 + GFF3387 0.62 -0.4 153,133 + GFF3387 0.84 -0.8 153,183 + -0.7 153,264 + -0.5 153,433 + GFF3388 0.36 -0.1 153,433 + GFF3388 0.36 +0.3 153,507 - GFF3388 0.47 -1.2 153,529 - GFF3388 0.51 +0.3 153,576 - GFF3388 0.58 +0.7 153,688 - GFF3388 0.76 +1.3 153,729 - GFF3388 0.82 -0.3 153,734 - GFF3388 0.83 -0.5 153,771 - GFF3388 0.89 -2.7 153,782 + -0.6 153,783 - -1.4 153,807 - -0.5 153,842 - +1.1 153,949 + GFF3389 0.15 +0.1 154,069 + GFF3389 0.30 -0.3 154,089 + GFF3389 0.32 +0.6 154,155 - GFF3389 0.40 +0.2 154,155 - GFF3389 0.40 -0.2 154,155 - GFF3389 0.40 -0.5
Or see this region's nucleotide sequence