Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF319 and GFF320 overlap by 23 nucleotides GFF320 and GFF321 overlap by 16 nucleotides GFF321 and GFF322 are separated by 71 nucleotides GFF322 and GFF323 are separated by 11 nucleotides
GFF319 - Beta-glucosidase (EC 3.2.1.21), at 35,753 to 37,135
GFF319
GFF320 - hypothetical protein, at 37,113 to 37,256
GFF320
GFF321 - Putative secreted protein, at 37,241 to 37,534
GFF321
GFF322 - hypothetical protein, at 37,606 to 37,719
GFF322
GFF323 - Transcriptional regulatory protein zraR, at 37,731 to 40,529
GFF323
Position (kb) 37
38 Strain fitness (log2 ratio) -2
-1
0
1 at 36.249 kb on + strand, within GFF319 at 36.249 kb on + strand, within GFF319 at 36.249 kb on + strand, within GFF319 at 36.250 kb on - strand, within GFF319 at 36.372 kb on - strand, within GFF319 at 36.476 kb on + strand, within GFF319 at 36.479 kb on + strand, within GFF319 at 36.480 kb on - strand, within GFF319 at 36.480 kb on - strand, within GFF319 at 36.682 kb on + strand, within GFF319 at 36.683 kb on - strand, within GFF319 at 36.683 kb on - strand, within GFF319 at 36.785 kb on - strand, within GFF319 at 36.897 kb on - strand, within GFF319 at 36.924 kb on - strand, within GFF319 at 36.963 kb on + strand, within GFF319 at 37.067 kb on - strand at 37.281 kb on + strand, within GFF321 at 37.281 kb on + strand, within GFF321 at 37.281 kb on + strand, within GFF321 at 37.282 kb on - strand, within GFF321 at 37.282 kb on - strand, within GFF321 at 37.570 kb on + strand at 37.571 kb on + strand at 37.571 kb on + strand at 37.577 kb on + strand at 37.686 kb on + strand, within GFF322 at 37.686 kb on + strand, within GFF322 at 37.687 kb on - strand, within GFF322 at 37.691 kb on + strand, within GFF322 at 37.692 kb on - strand, within GFF322 at 37.729 kb on + strand at 37.729 kb on + strand at 37.729 kb on + strand at 37.730 kb on - strand at 37.740 kb on - strand at 37.929 kb on + strand at 38.035 kb on - strand, within GFF323 at 38.134 kb on + strand, within GFF323 at 38.226 kb on - strand, within GFF323 at 38.268 kb on - strand, within GFF323 at 38.336 kb on + strand, within GFF323 at 38.337 kb on - strand, within GFF323 at 38.370 kb on + strand, within GFF323 at 38.403 kb on + strand, within GFF323 at 38.429 kb on + strand, within GFF323 at 38.430 kb on - strand, within GFF323 at 38.430 kb on - strand, within GFF323
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 36,249 + GFF319 0.36 +0.0 36,249 + GFF319 0.36 -0.3 36,249 + GFF319 0.36 +0.2 36,250 - GFF319 0.36 +0.2 36,372 - GFF319 0.45 -0.6 36,476 + GFF319 0.52 -0.2 36,479 + GFF319 0.52 +0.6 36,480 - GFF319 0.53 -0.5 36,480 - GFF319 0.53 -0.1 36,682 + GFF319 0.67 -1.2 36,683 - GFF319 0.67 +1.7 36,683 - GFF319 0.67 +0.2 36,785 - GFF319 0.75 -0.0 36,897 - GFF319 0.83 -0.2 36,924 - GFF319 0.85 -0.1 36,963 + GFF319 0.87 +0.9 37,067 - -0.2 37,281 + GFF321 0.14 -0.3 37,281 + GFF321 0.14 -0.3 37,281 + GFF321 0.14 -0.2 37,282 - GFF321 0.14 -0.9 37,282 - GFF321 0.14 -1.1 37,570 + +0.1 37,571 + -1.6 37,571 + +0.5 37,577 + -0.6 37,686 + GFF322 0.70 +0.0 37,686 + GFF322 0.70 +0.3 37,687 - GFF322 0.71 -0.3 37,691 + GFF322 0.75 +0.0 37,692 - GFF322 0.75 -0.1 37,729 + +0.5 37,729 + -0.4 37,729 + -0.3 37,730 - -0.7 37,740 - +0.2 37,929 + -1.0 38,035 - GFF323 0.11 +0.8 38,134 + GFF323 0.14 -2.4 38,226 - GFF323 0.18 +0.1 38,268 - GFF323 0.19 -0.3 38,336 + GFF323 0.22 -0.4 38,337 - GFF323 0.22 +0.5 38,370 + GFF323 0.23 -0.0 38,403 + GFF323 0.24 -0.0 38,429 + GFF323 0.25 +0.4 38,430 - GFF323 0.25 +0.5 38,430 - GFF323 0.25 +1.1
Or see this region's nucleotide sequence