Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3149 and GFF3150 are separated by 212 nucleotides GFF3150 and GFF3151 are separated by 5 nucleotides GFF3151 and GFF3152 are separated by 61 nucleotides GFF3152 and GFF3153 overlap by 2 nucleotides GFF3153 and GFF3154 are separated by 13 nucleotides
GFF3149 - Type I secretion outer membrane protein, TolC precursor, at 153,708 to 155,177
GFF3149
GFF3150 - UPF0441 protein ygiB, at 155,390 to 156,061
GFF3150
GFF3151 - Similarity with glutathionylspermidine synthase (EC 6.3.1.8), group 1, at 156,067 to 157,230
GFF3151
GFF3152 - Uncharacterized protein ygiD, at 157,292 to 158,080
GFF3152
GFF3153 - hypothetical protein, at 158,079 to 158,207
GFF3153
GFF3154 - Zinc transporter ZupT, at 158,221 to 158,994
GFF3154
Position (kb) 156
157
158 Strain fitness (log2 ratio) -2
-1
0
1
2 at 155.094 kb on + strand at 155.097 kb on - strand at 155.179 kb on - strand at 155.221 kb on + strand at 155.222 kb on - strand at 155.251 kb on + strand at 155.251 kb on + strand at 155.270 kb on - strand at 155.280 kb on - strand at 155.361 kb on + strand at 155.361 kb on + strand at 155.362 kb on - strand at 155.362 kb on - strand at 155.365 kb on + strand at 155.389 kb on + strand at 155.486 kb on - strand, within GFF3150 at 155.630 kb on - strand, within GFF3150 at 155.634 kb on - strand, within GFF3150 at 155.869 kb on + strand, within GFF3150 at 155.869 kb on + strand, within GFF3150 at 155.884 kb on + strand, within GFF3150 at 156.062 kb on + strand at 156.082 kb on - strand at 156.141 kb on + strand at 156.141 kb on + strand at 156.141 kb on + strand at 156.141 kb on + strand at 156.141 kb on + strand at 156.141 kb on + strand at 156.146 kb on - strand at 156.175 kb on + strand at 156.178 kb on + strand at 156.254 kb on + strand, within GFF3151 at 156.520 kb on - strand, within GFF3151 at 156.572 kb on - strand, within GFF3151 at 156.641 kb on + strand, within GFF3151 at 156.687 kb on + strand, within GFF3151 at 156.687 kb on + strand, within GFF3151 at 156.962 kb on - strand, within GFF3151 at 157.021 kb on - strand, within GFF3151 at 157.108 kb on + strand, within GFF3151 at 157.108 kb on + strand, within GFF3151 at 157.204 kb on + strand at 157.228 kb on + strand at 157.315 kb on + strand at 157.316 kb on - strand at 157.316 kb on - strand at 157.319 kb on + strand at 157.319 kb on + strand at 157.321 kb on + strand at 157.386 kb on - strand, within GFF3152 at 157.386 kb on - strand, within GFF3152 at 157.452 kb on + strand, within GFF3152 at 157.453 kb on - strand, within GFF3152 at 157.475 kb on + strand, within GFF3152 at 157.475 kb on + strand, within GFF3152 at 157.542 kb on - strand, within GFF3152 at 157.543 kb on + strand, within GFF3152 at 157.577 kb on - strand, within GFF3152 at 157.583 kb on + strand, within GFF3152 at 157.675 kb on + strand, within GFF3152 at 157.730 kb on + strand, within GFF3152 at 157.731 kb on - strand, within GFF3152 at 157.855 kb on - strand, within GFF3152 at 157.930 kb on + strand, within GFF3152 at 158.065 kb on + strand at 158.066 kb on - strand at 158.122 kb on + strand, within GFF3153 at 158.123 kb on - strand, within GFF3153 at 158.175 kb on + strand, within GFF3153 at 158.228 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 155,094 + +1.0 155,097 - +2.0 155,179 - +0.9 155,221 + -0.0 155,222 - -0.7 155,251 + +0.2 155,251 + +0.4 155,270 - -0.5 155,280 - -1.0 155,361 + -1.5 155,361 + +0.2 155,362 - -1.2 155,362 - -0.0 155,365 + +0.4 155,389 + +1.8 155,486 - GFF3150 0.14 -0.4 155,630 - GFF3150 0.36 +1.0 155,634 - GFF3150 0.36 -0.7 155,869 + GFF3150 0.71 -2.4 155,869 + GFF3150 0.71 -0.6 155,884 + GFF3150 0.74 -0.8 156,062 + -1.2 156,082 - -1.9 156,141 + +1.4 156,141 + -0.7 156,141 + -1.3 156,141 + -0.5 156,141 + -0.6 156,141 + -2.4 156,146 - -1.2 156,175 + -0.7 156,178 + -0.9 156,254 + GFF3151 0.16 -0.3 156,520 - GFF3151 0.39 -1.3 156,572 - GFF3151 0.43 -0.2 156,641 + GFF3151 0.49 -0.4 156,687 + GFF3151 0.53 -1.4 156,687 + GFF3151 0.53 -0.9 156,962 - GFF3151 0.77 -1.3 157,021 - GFF3151 0.82 -0.6 157,108 + GFF3151 0.89 -1.5 157,108 + GFF3151 0.89 -0.6 157,204 + -1.3 157,228 + -0.5 157,315 + +0.5 157,316 - -0.2 157,316 - -0.5 157,319 + +0.1 157,319 + +0.4 157,321 + -0.6 157,386 - GFF3152 0.12 +0.0 157,386 - GFF3152 0.12 -0.9 157,452 + GFF3152 0.20 +0.1 157,453 - GFF3152 0.20 +1.0 157,475 + GFF3152 0.23 +0.2 157,475 + GFF3152 0.23 +0.2 157,542 - GFF3152 0.32 +2.7 157,543 + GFF3152 0.32 -1.2 157,577 - GFF3152 0.36 +0.3 157,583 + GFF3152 0.37 -2.2 157,675 + GFF3152 0.49 +0.2 157,730 + GFF3152 0.56 -1.9 157,731 - GFF3152 0.56 -0.7 157,855 - GFF3152 0.71 -2.6 157,930 + GFF3152 0.81 +0.1 158,065 + +0.3 158,066 - -0.9 158,122 + GFF3153 0.33 +0.6 158,123 - GFF3153 0.34 -0.1 158,175 + GFF3153 0.74 -0.1 158,228 + -0.8
Or see this region's nucleotide sequence