Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2823 and GFF2824 are separated by 130 nucleotides GFF2824 and GFF2825 are separated by 5 nucleotides GFF2825 and GFF2826 are separated by 80 nucleotides GFF2826 and GFF2827 are separated by 3 nucleotides GFF2827 and GFF2828 are separated by 329 nucleotides
GFF2823 - DinG family ATP-dependent helicase YoaA, at 343,787 to 345,697
GFF2823
GFF2824 - RidA/YER057c/UK114 superfamily, group 2, YoaB-like protein, at 345,828 to 346,172
GFF2824
GFF2825 - FIG00638134: hypothetical protein, at 346,178 to 346,357
GFF2825
GFF2826 - Para-aminobenzoate synthase, aminase component (EC 2.6.1.85), at 346,438 to 347,802
GFF2826
GFF2827 - Hypothetical nudix hydrolase YeaB, at 347,806 to 348,384
GFF2827
GFF2828 - L-serine dehydratase, beta subunit (EC 4.3.1.17) / L-serine dehydratase, alpha subunit (EC 4.3.1.17), at 348,714 to 350,012
GFF2828
Position (kb) 346
347
348 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 345.534 kb on + strand at 345.535 kb on - strand at 345.535 kb on - strand at 345.540 kb on + strand at 345.540 kb on + strand at 345.541 kb on - strand at 345.726 kb on + strand at 345.727 kb on - strand at 345.746 kb on + strand at 345.822 kb on + strand at 345.822 kb on + strand at 345.823 kb on - strand at 345.826 kb on + strand at 345.826 kb on + strand at 345.827 kb on - strand at 345.880 kb on - strand, within GFF2824 at 345.884 kb on + strand, within GFF2824 at 346.112 kb on - strand, within GFF2824 at 346.276 kb on + strand, within GFF2825 at 346.276 kb on + strand, within GFF2825 at 346.362 kb on - strand at 346.372 kb on - strand at 346.390 kb on - strand at 346.417 kb on + strand at 346.638 kb on - strand, within GFF2826 at 346.641 kb on + strand, within GFF2826 at 346.782 kb on - strand, within GFF2826 at 346.810 kb on - strand, within GFF2826 at 346.909 kb on + strand, within GFF2826 at 347.265 kb on + strand, within GFF2826 at 347.617 kb on - strand, within GFF2826 at 347.659 kb on - strand, within GFF2826 at 347.659 kb on - strand, within GFF2826 at 347.659 kb on - strand, within GFF2826 at 347.659 kb on - strand, within GFF2826 at 347.804 kb on - strand at 347.885 kb on + strand, within GFF2827 at 348.159 kb on + strand, within GFF2827 at 348.316 kb on + strand, within GFF2827 at 348.388 kb on + strand at 348.389 kb on - strand at 348.464 kb on - strand at 348.466 kb on - strand at 348.466 kb on - strand at 348.466 kb on - strand at 348.467 kb on + strand at 348.467 kb on + strand at 348.468 kb on - strand at 348.468 kb on - strand at 348.468 kb on - strand at 348.472 kb on - strand at 348.521 kb on - strand at 348.586 kb on - strand at 348.601 kb on - strand at 348.602 kb on + strand at 348.603 kb on - strand at 348.641 kb on + strand at 348.641 kb on + strand at 348.645 kb on - strand at 348.671 kb on + strand at 348.672 kb on - strand at 348.725 kb on + strand at 348.760 kb on + strand at 348.796 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 345,534 + +0.3 345,535 - +0.4 345,535 - +0.3 345,540 + -0.3 345,540 + +0.8 345,541 - +0.1 345,726 + -0.5 345,727 - +0.7 345,746 + +1.1 345,822 + +1.8 345,822 + +0.8 345,823 - +0.1 345,826 + -0.3 345,826 + +0.4 345,827 - +0.5 345,880 - GFF2824 0.15 +1.0 345,884 + GFF2824 0.16 +0.7 346,112 - GFF2824 0.82 -0.6 346,276 + GFF2825 0.54 +0.8 346,276 + GFF2825 0.54 +0.2 346,362 - +1.0 346,372 - -1.0 346,390 - +0.1 346,417 + +2.6 346,638 - GFF2826 0.15 +1.4 346,641 + GFF2826 0.15 -0.6 346,782 - GFF2826 0.25 +0.4 346,810 - GFF2826 0.27 -0.2 346,909 + GFF2826 0.35 +1.2 347,265 + GFF2826 0.61 -0.1 347,617 - GFF2826 0.86 -0.6 347,659 - GFF2826 0.89 +0.6 347,659 - GFF2826 0.89 +2.0 347,659 - GFF2826 0.89 +0.1 347,659 - GFF2826 0.89 -0.7 347,804 - -0.1 347,885 + GFF2827 0.14 +0.1 348,159 + GFF2827 0.61 -0.2 348,316 + GFF2827 0.88 +0.9 348,388 + +0.2 348,389 - -0.0 348,464 - +0.9 348,466 - +0.6 348,466 - -0.8 348,466 - -1.7 348,467 + +0.2 348,467 + +0.4 348,468 - -0.1 348,468 - +0.7 348,468 - -0.8 348,472 - +0.3 348,521 - -2.1 348,586 - -1.3 348,601 - +0.2 348,602 + +0.4 348,603 - +1.1 348,641 + +0.6 348,641 + +2.9 348,645 - +0.4 348,671 + -0.8 348,672 - -0.2 348,725 + +1.0 348,760 + -0.7 348,796 - +1.0
Or see this region's nucleotide sequence