Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2675 and GFF2676 are separated by 189 nucleotides GFF2676 and GFF2677 overlap by 26 nucleotides GFF2677 and GFF2678 are separated by 115 nucleotides GFF2678 and GFF2679 are separated by 89 nucleotides
GFF2675 - Periplasmic Murein Peptide-Binding Protein MppA, at 197,615 to 199,228
GFF2675
GFF2676 - Gamma-D-Glutamyl-meso-Diaminopimelate Amidase, at 199,418 to 200,146
GFF2676
GFF2677 - L-alanine-DL-glutamate epimerase, at 200,121 to 201,086
GFF2677
GFF2678 - Thiol peroxidase, Tpx-type (EC 1.11.1.15), at 201,202 to 201,708
GFF2678
GFF2679 - Transcriptional repressor protein TyrR, at 201,798 to 203,339
GFF2679
Position (kb) 200
201
202 Strain fitness (log2 ratio) -2
-1
0
1
2 at 199.377 kb on + strand at 199.476 kb on - strand at 199.504 kb on - strand, within GFF2676 at 199.614 kb on + strand, within GFF2676 at 199.614 kb on + strand, within GFF2676 at 199.645 kb on - strand, within GFF2676 at 199.681 kb on - strand, within GFF2676 at 199.708 kb on - strand, within GFF2676 at 199.848 kb on + strand, within GFF2676 at 199.978 kb on - strand, within GFF2676 at 199.978 kb on - strand, within GFF2676 at 200.261 kb on + strand, within GFF2677 at 200.361 kb on - strand, within GFF2677 at 200.607 kb on - strand, within GFF2677 at 200.607 kb on - strand, within GFF2677 at 200.706 kb on + strand, within GFF2677 at 200.707 kb on - strand, within GFF2677 at 200.730 kb on - strand, within GFF2677 at 200.803 kb on - strand, within GFF2677 at 200.952 kb on - strand, within GFF2677 at 201.045 kb on - strand at 201.069 kb on + strand at 201.069 kb on + strand at 201.070 kb on - strand at 201.070 kb on - strand at 201.075 kb on - strand at 201.130 kb on + strand at 201.179 kb on + strand at 201.181 kb on + strand at 201.197 kb on + strand at 201.285 kb on + strand, within GFF2678 at 201.286 kb on - strand, within GFF2678 at 201.514 kb on - strand, within GFF2678 at 201.532 kb on - strand, within GFF2678 at 201.634 kb on - strand, within GFF2678 at 201.707 kb on - strand at 201.743 kb on - strand at 201.901 kb on + strand at 201.906 kb on - strand at 201.948 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 199,377 + +1.2 199,476 - -1.2 199,504 - GFF2676 0.12 +0.9 199,614 + GFF2676 0.27 +0.4 199,614 + GFF2676 0.27 +0.9 199,645 - GFF2676 0.31 +0.0 199,681 - GFF2676 0.36 +0.8 199,708 - GFF2676 0.40 +0.2 199,848 + GFF2676 0.59 -0.1 199,978 - GFF2676 0.77 +0.2 199,978 - GFF2676 0.77 +0.8 200,261 + GFF2677 0.14 +0.3 200,361 - GFF2677 0.25 -0.2 200,607 - GFF2677 0.50 +1.3 200,607 - GFF2677 0.50 -1.8 200,706 + GFF2677 0.61 -1.9 200,707 - GFF2677 0.61 -0.6 200,730 - GFF2677 0.63 -1.4 200,803 - GFF2677 0.71 -0.3 200,952 - GFF2677 0.86 +0.8 201,045 - +0.2 201,069 + -1.4 201,069 + -0.3 201,070 - -0.4 201,070 - -0.2 201,075 - -1.9 201,130 + -0.2 201,179 + -0.0 201,181 + +0.7 201,197 + +0.3 201,285 + GFF2678 0.16 +2.0 201,286 - GFF2678 0.17 -1.4 201,514 - GFF2678 0.62 -0.7 201,532 - GFF2678 0.65 -0.4 201,634 - GFF2678 0.85 +1.4 201,707 - -0.0 201,743 - +0.7 201,901 + +0.9 201,906 - -0.0 201,948 - -1.6
Or see this region's nucleotide sequence