Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2393 and GFF2394 overlap by 29 nucleotides GFF2394 and GFF2395 are separated by 26 nucleotides GFF2395 and GFF2396 are separated by 4 nucleotides
GFF2393 - Rossmann fold nucleotide-binding protein Smf possibly involved in DNA uptake, at 21,560 to 22,684
GFF2393
GFF2394 - Protein of unknown function Smg, at 22,656 to 23,129
GFF2394
GFF2395 - Similar to C-terminal Zn-finger domain of DNA topoisomerase I, at 23,156 to 23,698
GFF2395
GFF2396 - TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA, at 23,703 to 24,275
GFF2396
Position (kb)
22
23
24 Strain fitness (log2 ratio)
-2
-1
0
1 at 21.750 kb on + strand, within GFF2393 at 21.803 kb on + strand, within GFF2393 at 21.803 kb on + strand, within GFF2393 at 21.804 kb on - strand, within GFF2393 at 21.877 kb on + strand, within GFF2393 at 21.920 kb on + strand, within GFF2393 at 22.324 kb on + strand, within GFF2393 at 22.413 kb on - strand, within GFF2393 at 22.420 kb on - strand, within GFF2393 at 22.436 kb on - strand, within GFF2393 at 22.510 kb on - strand, within GFF2393 at 22.521 kb on + strand, within GFF2393 at 22.521 kb on + strand, within GFF2393 at 22.584 kb on + strand at 22.666 kb on + strand at 22.666 kb on + strand at 22.667 kb on - strand at 22.667 kb on - strand at 22.667 kb on - strand at 22.669 kb on + strand at 22.669 kb on + strand at 22.670 kb on - strand at 22.674 kb on + strand at 22.675 kb on - strand at 22.689 kb on + strand at 22.689 kb on + strand at 22.689 kb on + strand at 22.689 kb on + strand at 22.690 kb on - strand at 22.690 kb on - strand at 22.872 kb on + strand, within GFF2394 at 22.874 kb on + strand, within GFF2394 at 23.047 kb on + strand, within GFF2394 at 23.117 kb on + strand at 23.118 kb on - strand at 23.118 kb on - strand at 23.130 kb on + strand at 23.137 kb on + strand at 23.432 kb on - strand, within GFF2395 at 23.432 kb on - strand, within GFF2395 at 23.432 kb on - strand, within GFF2395
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 21,750 + GFF2393 0.17 +0.9 21,803 + GFF2393 0.22 +0.5 21,803 + GFF2393 0.22 +0.2 21,804 - GFF2393 0.22 +0.1 21,877 + GFF2393 0.28 +0.6 21,920 + GFF2393 0.32 +0.2 22,324 + GFF2393 0.68 -0.4 22,413 - GFF2393 0.76 -0.4 22,420 - GFF2393 0.76 +0.6 22,436 - GFF2393 0.78 -0.1 22,510 - GFF2393 0.84 -0.1 22,521 + GFF2393 0.85 +0.3 22,521 + GFF2393 0.85 -0.9 22,584 + -0.2 22,666 + -0.0 22,666 + -0.0 22,667 - +0.0 22,667 - +0.6 22,667 - -0.2 22,669 + -0.2 22,669 + +0.9 22,670 - +0.5 22,674 + +0.4 22,675 - -0.1 22,689 + +0.3 22,689 + +0.7 22,689 + +0.2 22,689 + -0.6 22,690 - -0.4 22,690 - -0.1 22,872 + GFF2394 0.46 +0.0 22,874 + GFF2394 0.46 +1.5 23,047 + GFF2394 0.82 -0.5 23,117 + +1.6 23,118 - +0.2 23,118 - -0.9 23,130 + +0.1 23,137 + -1.1 23,432 - GFF2395 0.51 -2.3 23,432 - GFF2395 0.51 -0.5 23,432 - GFF2395 0.51 +1.2
Or see this region's nucleotide sequence