Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2312 and GFF2313 are separated by 54 nucleotides GFF2313 and GFF2314 are separated by 9 nucleotides GFF2314 and GFF2315 are separated by 172 nucleotides
GFF2312 - 1,4-dihydroxy-2-naphthoate polyprenyltransferase (EC 2.5.1.74), at 97,078 to 98,019
GFF2312
GFF2313 - ATP-dependent hsl protease ATP-binding subunit HslU, at 98,074 to 99,405
GFF2313
GFF2314 - ATP-dependent protease HslV (EC 3.4.25.-), at 99,415 to 99,864
GFF2314
GFF2315 - Cell division protein FtsN, at 100,037 to 100,918
GFF2315
Position (kb) 98
99
100 Strain fitness (log2 ratio) -2
-1
0
1
2 at 97.118 kb on - strand at 97.195 kb on + strand, within GFF2312 at 97.196 kb on - strand, within GFF2312 at 97.276 kb on - strand, within GFF2312 at 97.303 kb on + strand, within GFF2312 at 97.387 kb on - strand, within GFF2312 at 97.389 kb on + strand, within GFF2312 at 97.393 kb on - strand, within GFF2312 at 97.407 kb on + strand, within GFF2312 at 97.407 kb on + strand, within GFF2312 at 97.410 kb on + strand, within GFF2312 at 97.477 kb on - strand, within GFF2312 at 97.490 kb on + strand, within GFF2312 at 97.511 kb on - strand, within GFF2312 at 97.511 kb on - strand, within GFF2312 at 97.511 kb on - strand, within GFF2312 at 97.583 kb on + strand, within GFF2312 at 97.645 kb on + strand, within GFF2312 at 97.683 kb on + strand, within GFF2312 at 97.783 kb on - strand, within GFF2312 at 97.809 kb on - strand, within GFF2312 at 97.886 kb on + strand, within GFF2312 at 98.077 kb on + strand at 98.077 kb on + strand at 98.078 kb on - strand at 98.078 kb on - strand at 98.092 kb on + strand at 98.135 kb on + strand at 98.135 kb on + strand at 98.136 kb on - strand at 98.281 kb on - strand, within GFF2313 at 98.281 kb on - strand, within GFF2313 at 98.502 kb on + strand, within GFF2313 at 98.503 kb on - strand, within GFF2313 at 98.503 kb on - strand, within GFF2313 at 98.564 kb on - strand, within GFF2313 at 98.576 kb on - strand, within GFF2313 at 98.576 kb on - strand, within GFF2313 at 98.619 kb on + strand, within GFF2313 at 98.643 kb on + strand, within GFF2313 at 98.764 kb on - strand, within GFF2313 at 98.802 kb on + strand, within GFF2313 at 98.854 kb on + strand, within GFF2313 at 99.004 kb on + strand, within GFF2313 at 99.004 kb on + strand, within GFF2313 at 99.004 kb on + strand, within GFF2313 at 99.004 kb on + strand, within GFF2313 at 99.004 kb on + strand, within GFF2313 at 99.111 kb on + strand, within GFF2313 at 99.111 kb on + strand, within GFF2313 at 99.111 kb on + strand, within GFF2313 at 99.111 kb on + strand, within GFF2313 at 99.219 kb on + strand, within GFF2313 at 99.294 kb on + strand at 99.294 kb on + strand at 99.416 kb on + strand at 99.472 kb on - strand, within GFF2314 at 99.587 kb on - strand, within GFF2314 at 99.596 kb on + strand, within GFF2314 at 99.685 kb on - strand, within GFF2314 at 99.685 kb on - strand, within GFF2314 at 99.756 kb on + strand, within GFF2314 at 99.756 kb on + strand, within GFF2314 at 99.932 kb on - strand at 99.932 kb on - strand at 99.934 kb on + strand at 99.934 kb on + strand at 99.934 kb on + strand at 99.935 kb on - strand at 99.965 kb on + strand at 99.965 kb on + strand at 99.965 kb on + strand at 99.966 kb on - strand at 100.002 kb on + strand at 100.003 kb on - strand at 100.012 kb on - strand at 100.012 kb on - strand at 100.012 kb on - strand at 100.014 kb on - strand at 100.014 kb on - strand at 100.069 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 97,118 - -0.0 97,195 + GFF2312 0.12 +1.0 97,196 - GFF2312 0.13 -0.6 97,276 - GFF2312 0.21 +1.8 97,303 + GFF2312 0.24 +1.9 97,387 - GFF2312 0.33 +0.6 97,389 + GFF2312 0.33 -0.1 97,393 - GFF2312 0.33 +0.1 97,407 + GFF2312 0.35 -0.6 97,407 + GFF2312 0.35 +1.0 97,410 + GFF2312 0.35 -1.3 97,477 - GFF2312 0.42 -0.1 97,490 + GFF2312 0.44 -0.7 97,511 - GFF2312 0.46 +1.0 97,511 - GFF2312 0.46 -0.2 97,511 - GFF2312 0.46 -0.3 97,583 + GFF2312 0.54 +0.2 97,645 + GFF2312 0.60 -0.1 97,683 + GFF2312 0.64 -0.5 97,783 - GFF2312 0.75 +1.2 97,809 - GFF2312 0.78 +0.8 97,886 + GFF2312 0.86 +1.1 98,077 + -0.2 98,077 + -1.0 98,078 - -0.4 98,078 - -0.3 98,092 + -1.5 98,135 + -1.6 98,135 + -1.4 98,136 - -0.7 98,281 - GFF2313 0.16 -0.6 98,281 - GFF2313 0.16 -1.8 98,502 + GFF2313 0.32 -0.1 98,503 - GFF2313 0.32 -1.5 98,503 - GFF2313 0.32 -0.7 98,564 - GFF2313 0.37 +1.1 98,576 - GFF2313 0.38 -1.5 98,576 - GFF2313 0.38 -0.1 98,619 + GFF2313 0.41 -2.5 98,643 + GFF2313 0.43 -1.0 98,764 - GFF2313 0.52 -1.2 98,802 + GFF2313 0.55 -0.2 98,854 + GFF2313 0.59 -1.1 99,004 + GFF2313 0.70 -0.5 99,004 + GFF2313 0.70 -1.5 99,004 + GFF2313 0.70 -0.5 99,004 + GFF2313 0.70 -0.9 99,004 + GFF2313 0.70 -0.9 99,111 + GFF2313 0.78 +1.0 99,111 + GFF2313 0.78 -1.1 99,111 + GFF2313 0.78 -1.1 99,111 + GFF2313 0.78 +1.7 99,219 + GFF2313 0.86 -0.4 99,294 + -1.2 99,294 + -0.1 99,416 + +0.4 99,472 - GFF2314 0.13 -1.1 99,587 - GFF2314 0.38 -0.3 99,596 + GFF2314 0.40 -0.7 99,685 - GFF2314 0.60 -1.3 99,685 - GFF2314 0.60 -0.8 99,756 + GFF2314 0.76 -0.7 99,756 + GFF2314 0.76 -1.3 99,932 - -0.7 99,932 - -0.4 99,934 + +0.4 99,934 + -1.9 99,934 + -2.4 99,935 - -1.6 99,965 + -1.1 99,965 + -0.6 99,965 + -1.2 99,966 - -0.7 100,002 + -0.2 100,003 - +0.7 100,012 - -0.6 100,012 - -0.0 100,012 - +0.8 100,014 - +1.1 100,014 - -0.4 100,069 + +1.0
Or see this region's nucleotide sequence