Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2184 and GFF2185 are separated by 26 nucleotides GFF2185 and GFF2186 are separated by 27 nucleotides GFF2186 and GFF2187 are separated by 3 nucleotides GFF2187 and GFF2188 are separated by 573 nucleotides
GFF2184 - Predicted P-loop ATPase fused to an acetyltransferase COG1444, at 30,290 to 32,308
GFF2184
GFF2185 - Putative inner membrane protein, at 32,335 to 32,535
GFF2185
GFF2186 - N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18), at 32,563 to 33,690
GFF2186
GFF2187 - FIG138056: a glutathione-dependent thiol reductase, at 33,694 to 34,050
GFF2187
GFF2188 - RND efflux system, inner membrane transporter CmeB, at 34,624 to 37,737
GFF2188
Position (kb) 32
33
34 Strain fitness (log2 ratio) -2
-1
0
1 at 31.563 kb on + strand, within GFF2184 at 31.563 kb on + strand, within GFF2184 at 31.563 kb on + strand, within GFF2184 at 32.099 kb on + strand, within GFF2184 at 32.191 kb on + strand at 32.540 kb on - strand at 33.720 kb on - strand at 33.921 kb on + strand, within GFF2187 at 33.940 kb on + strand, within GFF2187 at 34.322 kb on - strand at 34.416 kb on + strand at 34.428 kb on + strand at 34.437 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 31,563 + GFF2184 0.63 -0.4 31,563 + GFF2184 0.63 -0.1 31,563 + GFF2184 0.63 -1.6 32,099 + GFF2184 0.90 +0.5 32,191 + +0.1 32,540 - +0.0 33,720 - +0.5 33,921 + GFF2187 0.64 -1.1 33,940 + GFF2187 0.69 -2.3 34,322 - -0.1 34,416 + -0.8 34,428 + -0.1 34,437 + -0.2
Or see this region's nucleotide sequence