Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2138 and GFF2139 are separated by 106 nucleotides GFF2139 and GFF2140 are separated by 144 nucleotides
GFF2138 - Cytoplasmic axial filament protein CafA and Ribonuclease G (EC 3.1.4.-), at 25,603 to 27,072
GFF2138
GFF2139 - FIG005080: Possible exported protein, at 27,179 to 30,979
GFF2139
GFF2140 - TldD protein, part of TldE/TldD proteolytic complex, at 31,124 to 32,569
GFF2140
Position (kb)
27
28
29
30
31 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 26.190 kb on - strand, within GFF2138 at 26.318 kb on - strand, within GFF2138 at 26.318 kb on - strand, within GFF2138 at 26.347 kb on + strand, within GFF2138 at 26.367 kb on + strand, within GFF2138 at 26.380 kb on + strand, within GFF2138 at 26.381 kb on - strand, within GFF2138 at 26.449 kb on - strand, within GFF2138 at 26.449 kb on - strand, within GFF2138 at 26.511 kb on - strand, within GFF2138 at 26.511 kb on - strand, within GFF2138 at 26.514 kb on + strand, within GFF2138 at 26.514 kb on + strand, within GFF2138 at 26.545 kb on - strand, within GFF2138 at 26.658 kb on + strand, within GFF2138 at 26.731 kb on - strand, within GFF2138 at 26.888 kb on + strand, within GFF2138 at 26.905 kb on + strand, within GFF2138 at 27.039 kb on + strand at 27.094 kb on - strand at 27.394 kb on + strand at 27.784 kb on - strand, within GFF2139 at 28.186 kb on - strand, within GFF2139 at 28.632 kb on - strand, within GFF2139 at 28.802 kb on + strand, within GFF2139 at 28.829 kb on + strand, within GFF2139 at 28.830 kb on - strand, within GFF2139 at 28.832 kb on - strand, within GFF2139 at 28.869 kb on + strand, within GFF2139 at 29.024 kb on + strand, within GFF2139 at 29.145 kb on + strand, within GFF2139 at 29.170 kb on - strand, within GFF2139 at 29.233 kb on + strand, within GFF2139 at 29.233 kb on + strand, within GFF2139 at 29.248 kb on + strand, within GFF2139 at 29.369 kb on - strand, within GFF2139 at 29.416 kb on + strand, within GFF2139 at 29.416 kb on + strand, within GFF2139 at 29.435 kb on - strand, within GFF2139 at 29.445 kb on + strand, within GFF2139 at 29.534 kb on + strand, within GFF2139 at 29.534 kb on + strand, within GFF2139 at 29.737 kb on + strand, within GFF2139 at 29.772 kb on + strand, within GFF2139 at 30.052 kb on + strand, within GFF2139 at 30.052 kb on + strand, within GFF2139 at 30.053 kb on - strand, within GFF2139 at 30.056 kb on + strand, within GFF2139 at 30.057 kb on - strand, within GFF2139 at 30.062 kb on + strand, within GFF2139 at 30.062 kb on + strand, within GFF2139 at 30.065 kb on + strand, within GFF2139 at 30.139 kb on + strand, within GFF2139 at 30.211 kb on + strand, within GFF2139 at 30.212 kb on - strand, within GFF2139 at 30.358 kb on + strand, within GFF2139 at 30.359 kb on - strand, within GFF2139 at 30.359 kb on - strand, within GFF2139 at 30.361 kb on + strand, within GFF2139 at 30.361 kb on + strand, within GFF2139 at 30.426 kb on + strand, within GFF2139 at 30.553 kb on - strand, within GFF2139 at 30.587 kb on - strand, within GFF2139 at 30.611 kb on - strand at 30.640 kb on + strand at 30.640 kb on + strand at 30.640 kb on + strand at 30.647 kb on - strand at 30.676 kb on - strand at 31.014 kb on - strand at 31.017 kb on + strand at 31.017 kb on + strand at 31.018 kb on - strand at 31.061 kb on - strand at 31.069 kb on - strand at 31.113 kb on + strand at 31.141 kb on - strand at 31.141 kb on - strand at 31.289 kb on - strand, within GFF2140 at 31.301 kb on + strand, within GFF2140 at 31.302 kb on - strand, within GFF2140 at 31.303 kb on + strand, within GFF2140 at 31.374 kb on + strand, within GFF2140 at 31.608 kb on + strand, within GFF2140 at 31.608 kb on + strand, within GFF2140 at 31.618 kb on - strand, within GFF2140 at 31.751 kb on + strand, within GFF2140 at 31.962 kb on + strand, within GFF2140 at 31.962 kb on + strand, within GFF2140 at 31.963 kb on - strand, within GFF2140
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 26,190 - GFF2138 0.40 +0.8 26,318 - GFF2138 0.49 -0.4 26,318 - GFF2138 0.49 +1.1 26,347 + GFF2138 0.51 +0.1 26,367 + GFF2138 0.52 +0.3 26,380 + GFF2138 0.53 +0.1 26,381 - GFF2138 0.53 +0.3 26,449 - GFF2138 0.58 +0.6 26,449 - GFF2138 0.58 +0.3 26,511 - GFF2138 0.62 -0.5 26,511 - GFF2138 0.62 -0.9 26,514 + GFF2138 0.62 -0.5 26,514 + GFF2138 0.62 +0.0 26,545 - GFF2138 0.64 +0.4 26,658 + GFF2138 0.72 +1.1 26,731 - GFF2138 0.77 +0.4 26,888 + GFF2138 0.87 +1.5 26,905 + GFF2138 0.89 -0.2 27,039 + +0.5 27,094 - -0.5 27,394 + -1.4 27,784 - GFF2139 0.16 -0.2 28,186 - GFF2139 0.26 -0.8 28,632 - GFF2139 0.38 +0.5 28,802 + GFF2139 0.43 +0.0 28,829 + GFF2139 0.43 +0.6 28,830 - GFF2139 0.43 +0.8 28,832 - GFF2139 0.43 -0.2 28,869 + GFF2139 0.44 +0.0 29,024 + GFF2139 0.49 +0.3 29,145 + GFF2139 0.52 +0.2 29,170 - GFF2139 0.52 +0.5 29,233 + GFF2139 0.54 +0.1 29,233 + GFF2139 0.54 -0.3 29,248 + GFF2139 0.54 -0.2 29,369 - GFF2139 0.58 +0.4 29,416 + GFF2139 0.59 -0.4 29,416 + GFF2139 0.59 +2.6 29,435 - GFF2139 0.59 -0.2 29,445 + GFF2139 0.60 +0.3 29,534 + GFF2139 0.62 +0.4 29,534 + GFF2139 0.62 +0.4 29,737 + GFF2139 0.67 -0.1 29,772 + GFF2139 0.68 +0.1 30,052 + GFF2139 0.76 -0.1 30,052 + GFF2139 0.76 +0.3 30,053 - GFF2139 0.76 -0.1 30,056 + GFF2139 0.76 -0.9 30,057 - GFF2139 0.76 +0.1 30,062 + GFF2139 0.76 +0.2 30,062 + GFF2139 0.76 +0.2 30,065 + GFF2139 0.76 -0.9 30,139 + GFF2139 0.78 +0.2 30,211 + GFF2139 0.80 -0.0 30,212 - GFF2139 0.80 -1.3 30,358 + GFF2139 0.84 -0.0 30,359 - GFF2139 0.84 -1.7 30,359 - GFF2139 0.84 -1.3 30,361 + GFF2139 0.84 +2.6 30,361 + GFF2139 0.84 +1.0 30,426 + GFF2139 0.85 +0.0 30,553 - GFF2139 0.89 -0.2 30,587 - GFF2139 0.90 +0.3 30,611 - -1.2 30,640 + +1.1 30,640 + -0.6 30,640 + +0.4 30,647 - -0.1 30,676 - -0.4 31,014 - +0.8 31,017 + -0.0 31,017 + +0.7 31,018 - +0.7 31,061 - +0.4 31,069 - +0.1 31,113 + -0.7 31,141 - -0.6 31,141 - +1.0 31,289 - GFF2140 0.11 -0.2 31,301 + GFF2140 0.12 -0.0 31,302 - GFF2140 0.12 -1.2 31,303 + GFF2140 0.12 -0.9 31,374 + GFF2140 0.17 -0.2 31,608 + GFF2140 0.33 +0.5 31,608 + GFF2140 0.33 -3.7 31,618 - GFF2140 0.34 -0.1 31,751 + GFF2140 0.43 -0.7 31,962 + GFF2140 0.58 +0.1 31,962 + GFF2140 0.58 -2.0 31,963 - GFF2140 0.58 +1.1
Or see this region's nucleotide sequence