Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2104

Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2103 and GFF2104 are separated by 17 nucleotidesGFF2104 and GFF2105 are separated by 61 nucleotidesGFF2105 and GFF2106 overlap by 16 nucleotides GFF2103 - LysR family transcriptional regulator YfiE, at 11,530 to 12,429 GFF2103 GFF2104 - ATP-dependent RNA helicase SrmB, at 12,447 to 13,781 GFF2104 GFF2105 - tRNA (adenine37-N(6))-methyltransferase TrmN6 (EC 2.1.1.223), at 13,843 to 14,649 GFF2105 GFF2106 - L-aspartate oxidase (EC 1.4.3.16), at 14,634 to 16,256 GFF2106 Position (kb) 12 13 14Strain fitness (log2 ratio) -2 -1 0 1at 11.564 kb on + strandat 11.914 kb on + strand, within GFF2103at 11.973 kb on - strand, within GFF2103at 12.079 kb on - strand, within GFF2103at 12.190 kb on - strand, within GFF2103at 12.201 kb on + strand, within GFF2103at 12.240 kb on - strand, within GFF2103at 12.267 kb on + strand, within GFF2103at 12.371 kb on + strandat 12.375 kb on + strandat 12.375 kb on + strandat 12.375 kb on + strandat 12.376 kb on - strandat 12.442 kb on + strandat 12.442 kb on + strandat 12.669 kb on + strand, within GFF2104at 12.909 kb on - strand, within GFF2104at 12.912 kb on - strand, within GFF2104at 13.434 kb on - strand, within GFF2104at 13.434 kb on - strand, within GFF2104at 13.651 kb on - strandat 13.810 kb on + strandat 13.910 kb on - strandat 14.046 kb on - strand, within GFF2105at 14.125 kb on - strand, within GFF2105at 14.266 kb on - strand, within GFF2105at 14.268 kb on + strand, within GFF2105at 14.429 kb on + strand, within GFF2105at 14.600 kb on - strandat 14.613 kb on + strandat 14.635 kb on + strandat 14.635 kb on + strandat 14.639 kb on + strandat 14.702 kb on - strandat 14.711 kb on + strandat 14.736 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI
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11,564 + -0.3
11,914 + GFF2103 0.43 +1.1
11,973 - GFF2103 0.49 -0.2
12,079 - GFF2103 0.61 -0.1
12,190 - GFF2103 0.73 +0.3
12,201 + GFF2103 0.75 +0.2
12,240 - GFF2103 0.79 +0.4
12,267 + GFF2103 0.82 +0.2
12,371 + -0.1
12,375 + -0.4
12,375 + +0.7
12,375 + -0.1
12,376 - -0.2
12,442 + -0.4
12,442 + -0.3
12,669 + GFF2104 0.17 -1.8
12,909 - GFF2104 0.35 -1.1
12,912 - GFF2104 0.35 -0.8
13,434 - GFF2104 0.74 -1.6
13,434 - GFF2104 0.74 +0.2
13,651 - +1.1
13,810 + -0.3
13,910 - -0.3
14,046 - GFF2105 0.25 -0.6
14,125 - GFF2105 0.35 -0.1
14,266 - GFF2105 0.52 +1.5
14,268 + GFF2105 0.53 -0.2
14,429 + GFF2105 0.73 +0.1
14,600 - -0.0
14,613 + -0.5
14,635 + -1.0
14,635 + +1.2
14,639 + -1.8
14,702 - -0.6
14,711 + +0.0
14,736 - -0.2

Or see this region's nucleotide sequence