Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1912 and GFF1913 are separated by 142 nucleotides GFF1913 and GFF1914 are separated by 125 nucleotides GFF1914 and GFF1915 are separated by 51 nucleotides GFF1915 and GFF1916 are separated by 10 nucleotides
GFF1912 - Probable glutathione S-transferase (EC 2.5.1.18), YfcF homolog, at 106,016 to 106,660
GFF1912
GFF1913 - Probable glutathione S-transferase (EC 2.5.1.18), YfcG homolog, at 106,803 to 107,450
GFF1913
GFF1914 - Cell division inhibitor, at 107,576 to 108,469
GFF1914
GFF1915 - Histidine ABC transporter, ATP-binding protein HisP (TC 3.A.1.3.1), at 108,521 to 109,297
GFF1915
GFF1916 - Histidine ABC transporter, permease protein HisM (TC 3.A.1.3.1), at 109,308 to 110,015
GFF1916
Position (kb)
107
108
109 Strain fitness (log2 ratio)
-1
0
1
2 at 106.655 kb on - strand at 106.655 kb on - strand at 106.655 kb on - strand at 106.678 kb on + strand at 106.679 kb on + strand at 106.682 kb on + strand at 106.701 kb on + strand at 106.899 kb on - strand, within GFF1913 at 106.937 kb on + strand, within GFF1913 at 107.277 kb on + strand, within GFF1913 at 107.362 kb on - strand, within GFF1913 at 107.362 kb on - strand, within GFF1913 at 107.362 kb on - strand, within GFF1913 at 107.363 kb on + strand, within GFF1913 at 107.364 kb on - strand, within GFF1913 at 107.414 kb on - strand at 107.461 kb on + strand at 107.461 kb on + strand at 107.461 kb on + strand at 107.461 kb on + strand at 107.461 kb on + strand at 107.462 kb on - strand at 107.462 kb on - strand at 107.462 kb on - strand at 107.462 kb on - strand at 107.462 kb on - strand at 107.470 kb on + strand at 107.470 kb on + strand at 107.471 kb on - strand at 107.477 kb on + strand at 107.486 kb on - strand at 107.486 kb on - strand at 107.486 kb on - strand at 107.488 kb on - strand at 107.488 kb on - strand at 107.518 kb on - strand at 107.561 kb on + strand at 107.575 kb on + strand at 107.924 kb on + strand, within GFF1914 at 108.892 kb on - strand, within GFF1915 at 109.034 kb on + strand, within GFF1915 at 109.035 kb on - strand, within GFF1915 at 109.142 kb on - strand, within GFF1915 at 109.192 kb on + strand, within GFF1915 at 109.278 kb on - strand at 109.372 kb on + strand at 109.383 kb on + strand, within GFF1916 at 109.383 kb on + strand, within GFF1916 at 109.388 kb on - strand, within GFF1916 at 109.388 kb on - strand, within GFF1916 at 109.440 kb on - strand, within GFF1916
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 106,655 - -0.8 106,655 - -0.5 106,655 - +0.6 106,678 + +0.5 106,679 + +0.2 106,682 + -0.2 106,701 + -0.1 106,899 - GFF1913 0.15 -0.4 106,937 + GFF1913 0.21 -0.4 107,277 + GFF1913 0.73 -0.2 107,362 - GFF1913 0.86 +1.2 107,362 - GFF1913 0.86 -0.5 107,362 - GFF1913 0.86 +0.2 107,363 + GFF1913 0.86 -0.1 107,364 - GFF1913 0.87 -1.0 107,414 - +0.2 107,461 + +1.0 107,461 + +0.7 107,461 + -0.9 107,461 + +0.1 107,461 + -0.2 107,462 - +0.1 107,462 - -0.0 107,462 - -0.8 107,462 - -1.0 107,462 - +0.1 107,470 + +1.1 107,470 + +1.0 107,471 - -1.1 107,477 + -0.1 107,486 - +1.2 107,486 - +0.5 107,486 - -0.6 107,488 - +2.6 107,488 - +0.2 107,518 - -0.8 107,561 + +0.1 107,575 + +0.3 107,924 + GFF1914 0.39 -0.6 108,892 - GFF1915 0.48 +0.9 109,034 + GFF1915 0.66 +0.5 109,035 - GFF1915 0.66 -0.3 109,142 - GFF1915 0.80 -0.2 109,192 + GFF1915 0.86 -1.3 109,278 - +1.2 109,372 + +0.0 109,383 + GFF1916 0.11 -0.8 109,383 + GFF1916 0.11 +0.5 109,388 - GFF1916 0.11 +0.2 109,388 - GFF1916 0.11 -0.6 109,440 - GFF1916 0.19 -0.5
Or see this region's nucleotide sequence