Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF176 and GFF177 are separated by 340 nucleotides GFF177 and GFF178 are separated by 10 nucleotides
GFF176 - Arabinose operon regulatory protein, at 166,605 to 167,450
GFF176
GFF177 - Ribulokinase (EC 2.7.1.16), at 167,791 to 169,500
GFF177
GFF178 - L-arabinose isomerase (EC 5.3.1.4), at 169,511 to 171,013
GFF178
Position (kb) 167
168
169
170 Strain fitness (log2 ratio) -1
0
1 at 166.898 kb on - strand, within GFF176 at 166.898 kb on - strand, within GFF176 at 166.950 kb on - strand, within GFF176 at 166.961 kb on + strand, within GFF176 at 167.207 kb on - strand, within GFF176 at 167.238 kb on + strand, within GFF176 at 167.238 kb on + strand, within GFF176 at 167.316 kb on - strand, within GFF176 at 167.316 kb on - strand, within GFF176 at 167.554 kb on + strand at 167.631 kb on + strand at 167.633 kb on + strand at 167.633 kb on + strand at 167.882 kb on + strand at 167.883 kb on - strand at 167.960 kb on - strand at 168.006 kb on - strand, within GFF177 at 168.199 kb on - strand, within GFF177 at 168.242 kb on - strand, within GFF177 at 168.912 kb on - strand, within GFF177 at 169.215 kb on - strand, within GFF177 at 169.343 kb on + strand at 169.498 kb on + strand at 169.529 kb on + strand at 169.529 kb on + strand at 169.530 kb on - strand at 169.565 kb on + strand at 169.565 kb on + strand at 169.716 kb on - strand, within GFF178 at 169.792 kb on + strand, within GFF178 at 169.810 kb on - strand, within GFF178 at 169.870 kb on + strand, within GFF178 at 169.870 kb on + strand, within GFF178 at 169.871 kb on - strand, within GFF178 at 169.915 kb on + strand, within GFF178 at 169.970 kb on - strand, within GFF178 at 170.063 kb on - strand, within GFF178 at 170.093 kb on - strand, within GFF178 at 170.220 kb on - strand, within GFF178 at 170.342 kb on - strand, within GFF178
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 166,898 - GFF176 0.35 +0.2 166,898 - GFF176 0.35 +0.0 166,950 - GFF176 0.41 +0.1 166,961 + GFF176 0.42 -0.3 167,207 - GFF176 0.71 +0.1 167,238 + GFF176 0.75 +0.6 167,238 + GFF176 0.75 -0.2 167,316 - GFF176 0.84 +0.8 167,316 - GFF176 0.84 +0.0 167,554 + -0.7 167,631 + +0.2 167,633 + -0.3 167,633 + -1.6 167,882 + -1.0 167,883 - +0.1 167,960 - +0.8 168,006 - GFF177 0.13 +0.5 168,199 - GFF177 0.24 +0.7 168,242 - GFF177 0.26 -0.0 168,912 - GFF177 0.66 -0.2 169,215 - GFF177 0.83 +0.1 169,343 + +0.2 169,498 + -0.7 169,529 + +1.0 169,529 + +0.2 169,530 - -0.1 169,565 + -0.1 169,565 + -0.2 169,716 - GFF178 0.14 -0.7 169,792 + GFF178 0.19 -0.7 169,810 - GFF178 0.20 -1.3 169,870 + GFF178 0.24 -0.1 169,870 + GFF178 0.24 +1.6 169,871 - GFF178 0.24 +0.4 169,915 + GFF178 0.27 +1.5 169,970 - GFF178 0.31 -0.3 170,063 - GFF178 0.37 +0.2 170,093 - GFF178 0.39 -0.8 170,220 - GFF178 0.47 +0.5 170,342 - GFF178 0.55 +0.0
Or see this region's nucleotide sequence