Experiment: 3-methyl-1-butanol 0.4 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2462 and PP_2463 are separated by 200 nucleotides PP_2463 and PP_5525 are separated by 162 nucleotides PP_5525 and PP_2465 are separated by 338 nucleotides
PP_2462: PP_2462 - conserved protein of unknown function, at 2,808,000 to 2,808,374
_2462
PP_2463: PP_2463 - nucleic acid cold-shock chaperone, at 2,808,575 to 2,808,787
_2463
PP_5525: PP_5525 - conserved exported protein of unknown function, at 2,808,950 to 2,809,255
_5525
PP_2465: PP_2465 - Threonine--tRNA ligase, at 2,809,594 to 2,811,516
_2465
Position (kb)
2808
2809
2810 Strain fitness (log2 ratio)
-1
0
1 at 2808.073 kb on - strand, within PP_2462 at 2808.073 kb on - strand, within PP_2462 at 2808.431 kb on + strand at 2808.431 kb on + strand at 2808.431 kb on + strand at 2808.431 kb on + strand at 2808.432 kb on - strand at 2808.480 kb on + strand at 2808.567 kb on + strand at 2808.567 kb on + strand at 2808.568 kb on - strand at 2808.693 kb on - strand, within PP_2463 at 2808.808 kb on + strand at 2808.809 kb on - strand at 2808.866 kb on + strand at 2808.880 kb on + strand at 2808.880 kb on + strand at 2808.890 kb on + strand at 2808.891 kb on - strand at 2808.944 kb on - strand at 2809.155 kb on + strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.263 kb on + strand at 2809.264 kb on - strand at 2809.292 kb on + strand at 2809.292 kb on + strand at 2809.293 kb on - strand at 2809.332 kb on - strand at 2809.332 kb on - strand at 2809.389 kb on + strand at 2809.389 kb on + strand at 2809.430 kb on + strand at 2809.533 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 0.4 vol% remove 2,808,073 - PP_2462 0.19 -0.6 2,808,073 - PP_2462 0.19 -1.2 2,808,431 + +0.5 2,808,431 + +0.0 2,808,431 + -1.0 2,808,431 + +0.5 2,808,432 - +0.8 2,808,480 + -0.1 2,808,567 + -0.0 2,808,567 + -0.6 2,808,568 - +1.1 2,808,693 - PP_2463 0.55 -0.1 2,808,808 + -0.5 2,808,809 - -0.3 2,808,866 + +0.8 2,808,880 + -1.0 2,808,880 + +1.3 2,808,890 + +0.1 2,808,891 - +0.8 2,808,944 - -1.3 2,809,155 + PP_5525 0.67 -0.9 2,809,156 - PP_5525 0.67 +0.0 2,809,156 - PP_5525 0.67 -1.4 2,809,156 - PP_5525 0.67 -1.3 2,809,156 - PP_5525 0.67 -0.5 2,809,156 - PP_5525 0.67 -1.2 2,809,263 + +0.8 2,809,264 - +0.8 2,809,292 + +0.5 2,809,292 + +0.4 2,809,293 - -1.4 2,809,332 - -1.5 2,809,332 - +0.8 2,809,389 + -1.1 2,809,389 + +0.0 2,809,430 + +0.5 2,809,533 + +0.9
Or see this region's nucleotide sequence