Experiment: 3-methyl-1-butanol 0.4 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2524 and PP_2525 are separated by 31 nucleotides PP_2525 and PP_2526 are separated by 146 nucleotides PP_2526 and PP_2527 are separated by 81 nucleotides
PP_2524: PP_2524 - HD domain protein, at 2,871,223 to 2,871,879
_2524
PP_2525: PP_2525 - conserved protein of unknown function, at 2,871,911 to 2,873,074
_2525
PP_2526: PP_2526 - conserved membrane protein of unknown function, at 2,873,221 to 2,873,694
_2526
PP_2527: PP_2527 - conserved membrane protein of unknown function, at 2,873,776 to 2,874,540
_2527
Position (kb)
2871
2872
2873
2874 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2870.928 kb on + strand at 2870.929 kb on - strand at 2870.929 kb on - strand at 2870.930 kb on + strand at 2870.930 kb on + strand at 2871.056 kb on - strand at 2871.056 kb on - strand at 2871.079 kb on + strand at 2871.120 kb on + strand at 2871.120 kb on + strand at 2871.153 kb on + strand at 2871.215 kb on + strand at 2871.215 kb on + strand at 2871.216 kb on - strand at 2871.247 kb on + strand at 2871.277 kb on + strand at 2871.278 kb on - strand at 2871.321 kb on + strand, within PP_2524 at 2871.440 kb on + strand at 2871.440 kb on + strand, within PP_2524 at 2871.441 kb on - strand, within PP_2524 at 2871.527 kb on + strand, within PP_2524 at 2871.528 kb on - strand, within PP_2524 at 2871.554 kb on - strand, within PP_2524 at 2871.652 kb on + strand, within PP_2524 at 2871.683 kb on - strand, within PP_2524 at 2871.683 kb on - strand, within PP_2524 at 2871.783 kb on + strand, within PP_2524 at 2871.784 kb on - strand, within PP_2524 at 2871.784 kb on - strand, within PP_2524 at 2871.894 kb on + strand at 2871.895 kb on - strand at 2871.992 kb on + strand at 2871.993 kb on - strand at 2872.050 kb on - strand, within PP_2525 at 2872.265 kb on + strand, within PP_2525 at 2872.266 kb on - strand, within PP_2525 at 2872.695 kb on - strand, within PP_2525 at 2873.046 kb on + strand at 2873.065 kb on + strand at 2873.066 kb on - strand at 2873.183 kb on + strand at 2873.183 kb on + strand at 2873.183 kb on + strand at 2873.184 kb on - strand at 2873.185 kb on + strand at 2873.186 kb on - strand at 2873.186 kb on - strand at 2873.448 kb on - strand, within PP_2526 at 2873.596 kb on + strand, within PP_2526 at 2873.596 kb on + strand, within PP_2526 at 2873.605 kb on + strand, within PP_2526 at 2873.665 kb on + strand at 2873.666 kb on - strand at 2873.666 kb on - strand at 2873.666 kb on - strand at 2873.891 kb on + strand, within PP_2527 at 2873.891 kb on + strand, within PP_2527
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 0.4 vol% remove 2,870,928 + +0.2 2,870,929 - -0.8 2,870,929 - -0.1 2,870,930 + -2.8 2,870,930 + +0.8 2,871,056 - +1.7 2,871,056 - +1.0 2,871,079 + +0.1 2,871,120 + +0.5 2,871,120 + +1.1 2,871,153 + -0.4 2,871,215 + -0.6 2,871,215 + -0.1 2,871,216 - +0.5 2,871,247 + +0.1 2,871,277 + +1.4 2,871,278 - +0.3 2,871,321 + PP_2524 0.15 +1.0 2,871,440 + +2.1 2,871,440 + PP_2524 0.33 +0.3 2,871,441 - PP_2524 0.33 +0.9 2,871,527 + PP_2524 0.46 +0.1 2,871,528 - PP_2524 0.46 -1.0 2,871,554 - PP_2524 0.50 +1.0 2,871,652 + PP_2524 0.65 -0.3 2,871,683 - PP_2524 0.70 -0.8 2,871,683 - PP_2524 0.70 +0.8 2,871,783 + PP_2524 0.85 -0.3 2,871,784 - PP_2524 0.85 +0.2 2,871,784 - PP_2524 0.85 -0.3 2,871,894 + +0.9 2,871,895 - +1.1 2,871,992 + -0.7 2,871,993 - -0.6 2,872,050 - PP_2525 0.12 -0.5 2,872,265 + PP_2525 0.30 -0.1 2,872,266 - PP_2525 0.30 -0.6 2,872,695 - PP_2525 0.67 -1.3 2,873,046 + -1.4 2,873,065 + -0.3 2,873,066 - -0.5 2,873,183 + +0.8 2,873,183 + +0.1 2,873,183 + -0.3 2,873,184 - +2.2 2,873,185 + +1.2 2,873,186 - -0.5 2,873,186 - +1.9 2,873,448 - PP_2526 0.48 +1.0 2,873,596 + PP_2526 0.79 -0.2 2,873,596 + PP_2526 0.79 -0.1 2,873,605 + PP_2526 0.81 -0.3 2,873,665 + -0.4 2,873,666 - -0.6 2,873,666 - +0.1 2,873,666 - +0.5 2,873,891 + PP_2527 0.15 +0.2 2,873,891 + PP_2527 0.15 -0.6
Or see this region's nucleotide sequence