Experiment: 3-methyl-1-butanol 0.4 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1985 and PP_1986 overlap by 4 nucleotides PP_1986 and PP_1987 are separated by 118 nucleotides PP_1987 and PP_1988 are separated by 54 nucleotides
PP_1985: PP_1985 - 3-isopropylmalate dehydratase large subunit, at 2,250,670 to 2,252,103
_1985
PP_1986: PP_1986 - 3-isopropylmalate dehydratase small subunit, at 2,252,100 to 2,252,744
_1986
PP_1987: PP_1987 - Methlytransferase, UbiE/COQ5 family, at 2,252,863 to 2,253,627
_1987
PP_1988: PP_1988 - 3-isopropylmalate dehydrogenase, at 2,253,682 to 2,254,764
_1988
Position (kb)
2252
2253
2254 Strain fitness (log2 ratio)
-2
-1
0
1 at 2252.241 kb on + strand, within PP_1986 at 2252.242 kb on - strand, within PP_1986 at 2252.263 kb on - strand, within PP_1986 at 2252.320 kb on - strand, within PP_1986 at 2252.403 kb on + strand, within PP_1986 at 2252.780 kb on - strand at 2252.784 kb on - strand at 2253.076 kb on + strand, within PP_1987 at 2253.385 kb on + strand, within PP_1987 at 2253.555 kb on - strand at 2253.562 kb on + strand at 2253.563 kb on - strand at 2253.563 kb on - strand at 2253.563 kb on - strand at 2253.565 kb on + strand at 2253.752 kb on + strand at 2254.102 kb on + strand, within PP_1988 at 2254.156 kb on + strand, within PP_1988 at 2254.157 kb on - strand, within PP_1988 at 2254.157 kb on - strand, within PP_1988 at 2254.157 kb on - strand, within PP_1988 at 2254.172 kb on - strand, within PP_1988 at 2254.345 kb on + strand, within PP_1988 at 2254.345 kb on + strand, within PP_1988 at 2254.345 kb on + strand, within PP_1988 at 2254.345 kb on + strand, within PP_1988 at 2254.346 kb on - strand, within PP_1988 at 2254.346 kb on - strand, within PP_1988 at 2254.346 kb on - strand, within PP_1988 at 2254.346 kb on - strand, within PP_1988 at 2254.510 kb on + strand, within PP_1988 at 2254.511 kb on - strand, within PP_1988 at 2254.511 kb on - strand, within PP_1988 at 2254.511 kb on - strand, within PP_1988 at 2254.511 kb on - strand, within PP_1988 at 2254.511 kb on - strand, within PP_1988 at 2254.603 kb on + strand, within PP_1988
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 0.4 vol% remove 2,252,241 + PP_1986 0.22 +0.7 2,252,242 - PP_1986 0.22 -0.1 2,252,263 - PP_1986 0.25 -0.8 2,252,320 - PP_1986 0.34 -1.1 2,252,403 + PP_1986 0.47 -0.4 2,252,780 - +1.1 2,252,784 - -0.1 2,253,076 + PP_1987 0.28 -1.5 2,253,385 + PP_1987 0.68 -1.0 2,253,555 - -0.8 2,253,562 + +0.1 2,253,563 - +0.1 2,253,563 - -0.9 2,253,563 - -2.7 2,253,565 + +0.3 2,253,752 + -0.9 2,254,102 + PP_1988 0.39 +0.5 2,254,156 + PP_1988 0.44 -0.0 2,254,157 - PP_1988 0.44 +0.6 2,254,157 - PP_1988 0.44 +1.0 2,254,157 - PP_1988 0.44 -1.5 2,254,172 - PP_1988 0.45 -0.9 2,254,345 + PP_1988 0.61 +1.0 2,254,345 + PP_1988 0.61 +0.4 2,254,345 + PP_1988 0.61 +0.1 2,254,345 + PP_1988 0.61 -0.2 2,254,346 - PP_1988 0.61 +0.9 2,254,346 - PP_1988 0.61 -0.3 2,254,346 - PP_1988 0.61 +0.0 2,254,346 - PP_1988 0.61 -0.4 2,254,510 + PP_1988 0.76 -0.2 2,254,511 - PP_1988 0.77 +0.5 2,254,511 - PP_1988 0.77 -1.1 2,254,511 - PP_1988 0.77 -1.2 2,254,511 - PP_1988 0.77 +0.6 2,254,511 - PP_1988 0.77 +0.1 2,254,603 + PP_1988 0.85 +0.0
Or see this region's nucleotide sequence