Strain Fitness in Pseudomonas putida KT2440 around PP_1311

Experiment: 3-methyl-1-butanol 0.4 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1309 and PP_1310 are separated by 135 nucleotidesPP_1310 and PP_1311 are separated by 25 nucleotidesPP_1311 and PP_1312 are separated by 163 nucleotides PP_1309: PP_1309 - conserved exported protein of unknown function, at 1,496,840 to 1,497,277 _1309 PP_1310: PP_1310 - conserved hypothetical protein, at 1,497,413 to 1,498,048 _1310 PP_1311: PP_1311 - Tryptophan--tRNA ligase, at 1,498,074 to 1,499,423 _1311 PP_1312: PP_1312 - putative enzyme with nucleoside triphosphate hydrolase domain, at 1,499,587 to 1,500,681 _1312 Position (kb) 1498 1499 1500Strain fitness (log2 ratio) -1 0 1 2 3at 1497.126 kb on + strand, within PP_1309at 1497.126 kb on + strand, within PP_1309at 1497.126 kb on + strand, within PP_1309at 1497.290 kb on + strandat 1497.291 kb on - strandat 1497.291 kb on - strandat 1497.291 kb on - strandat 1497.292 kb on + strandat 1497.307 kb on + strandat 1497.333 kb on + strandat 1497.333 kb on + strandat 1497.334 kb on - strandat 1497.334 kb on - strandat 1497.334 kb on - strandat 1497.375 kb on - strandat 1497.406 kb on + strandat 1497.407 kb on - strandat 1497.473 kb on + strandat 1497.614 kb on + strand, within PP_1310at 1497.614 kb on + strand, within PP_1310at 1497.615 kb on - strand, within PP_1310at 1497.926 kb on + strand, within PP_1310at 1497.927 kb on - strand, within PP_1310at 1499.541 kb on + strandat 1500.095 kb on - strand, within PP_1312at 1500.352 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 0.4 vol%
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1,497,126 + PP_1309 0.65 +1.9
1,497,126 + PP_1309 0.65 +2.9
1,497,126 + PP_1309 0.65 +2.2
1,497,290 + +2.5
1,497,291 - +1.5
1,497,291 - +2.1
1,497,291 - +2.4
1,497,292 + +2.5
1,497,307 + +2.3
1,497,333 + +1.9
1,497,333 + +2.8
1,497,334 - +0.1
1,497,334 - +0.8
1,497,334 - +0.3
1,497,375 - +1.1
1,497,406 + +1.4
1,497,407 - +0.9
1,497,473 + +1.2
1,497,614 + PP_1310 0.32 +0.4
1,497,614 + PP_1310 0.32 -0.4
1,497,615 - PP_1310 0.32 +2.4
1,497,926 + PP_1310 0.81 -0.4
1,497,927 - PP_1310 0.81 +1.0
1,499,541 + -1.4
1,500,095 - PP_1312 0.46 -0.4
1,500,352 - -0.1

Or see this region's nucleotide sequence