Strain Fitness in Pseudomonas putida KT2440 around PP_1309

Experiment: 3-methyl-1-butanol 0.4 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1308 and PP_1309 are separated by 71 nucleotidesPP_1309 and PP_1310 are separated by 135 nucleotidesPP_1310 and PP_1311 are separated by 25 nucleotides PP_1308: PP_1308 - methionine gamma-lyase, at 1,495,572 to 1,496,768 _1308 PP_1309: PP_1309 - conserved exported protein of unknown function, at 1,496,840 to 1,497,277 _1309 PP_1310: PP_1310 - conserved hypothetical protein, at 1,497,413 to 1,498,048 _1310 PP_1311: PP_1311 - Tryptophan--tRNA ligase, at 1,498,074 to 1,499,423 _1311 Position (kb) 1496 1497 1498Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1495.850 kb on + strand, within PP_1308at 1495.850 kb on + strand, within PP_1308at 1495.850 kb on + strand, within PP_1308at 1495.911 kb on + strand, within PP_1308at 1495.920 kb on - strand, within PP_1308at 1495.920 kb on - strand, within PP_1308at 1495.943 kb on + strand, within PP_1308at 1496.034 kb on + strand, within PP_1308at 1496.034 kb on + strand, within PP_1308at 1496.141 kb on + strand, within PP_1308at 1496.141 kb on + strand, within PP_1308at 1496.141 kb on + strand, within PP_1308at 1496.142 kb on - strand, within PP_1308at 1496.142 kb on - strand, within PP_1308at 1496.148 kb on + strand, within PP_1308at 1496.225 kb on + strand, within PP_1308at 1496.226 kb on - strand, within PP_1308at 1496.226 kb on - strandat 1496.294 kb on + strand, within PP_1308at 1496.294 kb on + strand, within PP_1308at 1496.294 kb on + strand, within PP_1308at 1496.646 kb on + strand, within PP_1308at 1496.646 kb on + strand, within PP_1308at 1496.647 kb on - strand, within PP_1308at 1496.647 kb on - strand, within PP_1308at 1496.648 kb on + strand, within PP_1308at 1496.766 kb on + strandat 1496.885 kb on - strand, within PP_1309at 1496.885 kb on - strand, within PP_1309at 1496.902 kb on - strand, within PP_1309at 1496.929 kb on + strand, within PP_1309at 1496.930 kb on - strand, within PP_1309at 1496.930 kb on - strand, within PP_1309at 1497.050 kb on + strand, within PP_1309at 1497.060 kb on + strandat 1497.060 kb on + strand, within PP_1309at 1497.060 kb on + strand, within PP_1309at 1497.061 kb on - strand, within PP_1309at 1497.061 kb on - strand, within PP_1309at 1497.126 kb on + strand, within PP_1309at 1497.126 kb on + strand, within PP_1309at 1497.126 kb on + strand, within PP_1309at 1497.290 kb on + strandat 1497.291 kb on - strandat 1497.291 kb on - strandat 1497.291 kb on - strandat 1497.292 kb on + strandat 1497.307 kb on + strandat 1497.333 kb on + strandat 1497.333 kb on + strandat 1497.334 kb on - strandat 1497.334 kb on - strandat 1497.334 kb on - strandat 1497.375 kb on - strandat 1497.406 kb on + strandat 1497.407 kb on - strandat 1497.473 kb on + strandat 1497.614 kb on + strand, within PP_1310at 1497.614 kb on + strand, within PP_1310at 1497.615 kb on - strand, within PP_1310at 1497.926 kb on + strand, within PP_1310at 1497.927 kb on - strand, within PP_1310

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 0.4 vol%
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1,495,850 + PP_1308 0.23 +0.5
1,495,850 + PP_1308 0.23 +0.1
1,495,850 + PP_1308 0.23 +1.9
1,495,911 + PP_1308 0.28 -2.5
1,495,920 - PP_1308 0.29 +0.8
1,495,920 - PP_1308 0.29 -1.4
1,495,943 + PP_1308 0.31 -0.3
1,496,034 + PP_1308 0.39 -0.0
1,496,034 + PP_1308 0.39 +1.0
1,496,141 + PP_1308 0.48 +0.3
1,496,141 + PP_1308 0.48 +0.5
1,496,141 + PP_1308 0.48 +0.4
1,496,142 - PP_1308 0.48 +0.3
1,496,142 - PP_1308 0.48 -1.6
1,496,148 + PP_1308 0.48 -0.9
1,496,225 + PP_1308 0.55 -3.3
1,496,226 - PP_1308 0.55 +0.4
1,496,226 - +0.1
1,496,294 + PP_1308 0.60 +0.4
1,496,294 + PP_1308 0.60 +0.4
1,496,294 + PP_1308 0.60 -0.6
1,496,646 + PP_1308 0.90 +0.2
1,496,646 + PP_1308 0.90 -0.7
1,496,647 - PP_1308 0.90 -0.6
1,496,647 - PP_1308 0.90 +0.3
1,496,648 + PP_1308 0.90 -0.1
1,496,766 + -0.1
1,496,885 - PP_1309 0.10 +1.0
1,496,885 - PP_1309 0.10 +0.5
1,496,902 - PP_1309 0.14 +2.7
1,496,929 + PP_1309 0.20 +0.8
1,496,930 - PP_1309 0.21 +3.3
1,496,930 - PP_1309 0.21 +2.1
1,497,050 + PP_1309 0.48 +2.5
1,497,060 + +2.9
1,497,060 + PP_1309 0.50 +3.3
1,497,060 + PP_1309 0.50 +2.6
1,497,061 - PP_1309 0.50 +2.7
1,497,061 - PP_1309 0.50 +2.6
1,497,126 + PP_1309 0.65 +1.9
1,497,126 + PP_1309 0.65 +2.9
1,497,126 + PP_1309 0.65 +2.2
1,497,290 + +2.5
1,497,291 - +1.5
1,497,291 - +2.1
1,497,291 - +2.4
1,497,292 + +2.5
1,497,307 + +2.3
1,497,333 + +1.9
1,497,333 + +2.8
1,497,334 - +0.1
1,497,334 - +0.8
1,497,334 - +0.3
1,497,375 - +1.1
1,497,406 + +1.4
1,497,407 - +0.9
1,497,473 + +1.2
1,497,614 + PP_1310 0.32 +0.4
1,497,614 + PP_1310 0.32 -0.4
1,497,615 - PP_1310 0.32 +2.4
1,497,926 + PP_1310 0.81 -0.4
1,497,927 - PP_1310 0.81 +1.0

Or see this region's nucleotide sequence