Experiment: 3-methyl-1-butanol 0.4 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0399 and PP_0400 overlap by 1 nucleotides PP_0400 and PP_0401 are separated by 197 nucleotides PP_0401 and PP_0402 overlap by 4 nucleotides PP_0402 and PP_0403 overlap by 4 nucleotides
PP_0399: PP_0399 - Bis(5'-nucleosyl)-tetraphosphatase, symmetrical, at 485,068 to 485,934
_0399
PP_0400: PP_0400 - CO2+/MG2+ efflux protein, at 485,934 to 486,314
_0400
PP_0401: PP_0401 - Ribosomal RNA small subunit methyltransferase A, at 486,512 to 487,315
_0401
PP_0402: PP_0402 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 487,312 to 488,301
_0402
PP_0403: PP_0403 - peptidyl-prolyl cis-trans isomerase, at 488,298 to 489,617
_0403
Position (kb) 486
487
488 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 486.017 kb on - strand, within PP_0400 at 486.067 kb on + strand, within PP_0400 at 486.068 kb on - strand, within PP_0400 at 486.068 kb on - strand, within PP_0400 at 486.068 kb on - strand, within PP_0400 at 486.068 kb on - strand, within PP_0400 at 486.126 kb on + strand, within PP_0400 at 486.126 kb on + strand, within PP_0400 at 486.127 kb on - strand, within PP_0400 at 486.136 kb on - strand, within PP_0400 at 486.136 kb on - strand, within PP_0400 at 486.220 kb on + strand, within PP_0400 at 486.221 kb on - strand, within PP_0400 at 486.343 kb on - strand at 486.343 kb on - strand at 486.343 kb on - strand at 486.353 kb on - strand at 486.428 kb on + strand at 486.440 kb on + strand at 486.508 kb on + strand at 486.829 kb on - strand, within PP_0401 at 486.829 kb on - strand, within PP_0401 at 486.978 kb on + strand, within PP_0401 at 486.978 kb on + strand, within PP_0401 at 486.979 kb on - strand, within PP_0401 at 486.979 kb on - strand, within PP_0401 at 486.979 kb on - strand, within PP_0401 at 487.302 kb on + strand at 487.303 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 0.4 vol% remove 486,017 - PP_0400 0.22 +1.4 486,067 + PP_0400 0.35 +2.2 486,068 - PP_0400 0.35 +0.2 486,068 - PP_0400 0.35 -3.5 486,068 - PP_0400 0.35 +2.1 486,068 - PP_0400 0.35 -0.4 486,126 + PP_0400 0.50 -0.4 486,126 + PP_0400 0.50 -1.7 486,127 - PP_0400 0.51 -0.3 486,136 - PP_0400 0.53 -0.1 486,136 - PP_0400 0.53 +0.8 486,220 + PP_0400 0.75 -0.8 486,221 - PP_0400 0.75 -0.6 486,343 - -0.4 486,343 - -0.8 486,343 - -0.7 486,353 - -0.4 486,428 + -0.4 486,440 + +1.2 486,508 + +0.7 486,829 - PP_0401 0.39 -1.2 486,829 - PP_0401 0.39 -0.2 486,978 + PP_0401 0.58 +0.6 486,978 + PP_0401 0.58 -0.1 486,979 - PP_0401 0.58 -1.5 486,979 - PP_0401 0.58 -0.1 486,979 - PP_0401 0.58 -0.7 487,302 + -1.7 487,303 - +0.6
Or see this region's nucleotide sequence