Experiment: 3-methyl-1-butanol 0.4 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0243 and PP_0244 are separated by 153 nucleotides PP_0244 and PP_0245 overlap by 1 nucleotides
PP_0243: PP_0243 - Glutamate--cysteine ligase, at 296,426 to 298,003
_0243
PP_0244: PP_0244 - conserved protein of unknown function, at 298,157 to 298,540
_0244
PP_0245: PP_0245 - putative transcriptional accessory protein, at 298,540 to 301,125
_0245
Position (kb) 298
299 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 298.008 kb on + strand at 298.009 kb on - strand at 298.049 kb on + strand at 298.057 kb on + strand at 298.057 kb on + strand at 298.066 kb on - strand at 298.066 kb on - strand at 298.066 kb on - strand at 298.143 kb on + strand at 298.144 kb on - strand at 298.158 kb on + strand at 298.158 kb on + strand at 298.158 kb on + strand at 298.159 kb on - strand at 298.159 kb on - strand at 298.211 kb on + strand, within PP_0244 at 298.211 kb on + strand at 298.211 kb on + strand, within PP_0244 at 298.211 kb on + strand, within PP_0244 at 298.212 kb on - strand, within PP_0244 at 298.299 kb on + strand, within PP_0244 at 298.299 kb on + strand, within PP_0244 at 298.299 kb on + strand, within PP_0244 at 298.299 kb on + strand, within PP_0244 at 298.300 kb on - strand, within PP_0244 at 298.521 kb on + strand at 298.522 kb on - strand at 298.532 kb on + strand at 298.532 kb on + strand at 298.533 kb on - strand at 298.541 kb on + strand at 298.541 kb on + strand at 298.541 kb on + strand at 298.542 kb on - strand at 298.833 kb on + strand, within PP_0245 at 298.834 kb on - strand, within PP_0245 at 298.834 kb on - strand at 298.888 kb on - strand, within PP_0245 at 299.022 kb on - strand, within PP_0245 at 299.462 kb on + strand, within PP_0245 at 299.462 kb on + strand, within PP_0245 at 299.463 kb on - strand, within PP_0245
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 0.4 vol% remove 298,008 + +0.3 298,009 - -0.6 298,049 + -0.9 298,057 + +1.7 298,057 + -2.8 298,066 - +1.0 298,066 - +1.9 298,066 - +0.3 298,143 + -4.3 298,144 - -1.4 298,158 + -1.3 298,158 + -0.2 298,158 + -1.0 298,159 - -0.0 298,159 - -0.4 298,211 + PP_0244 0.14 -1.3 298,211 + -0.5 298,211 + PP_0244 0.14 -0.2 298,211 + PP_0244 0.14 -1.4 298,212 - PP_0244 0.14 -0.4 298,299 + PP_0244 0.37 -0.1 298,299 + PP_0244 0.37 +0.0 298,299 + PP_0244 0.37 -2.0 298,299 + PP_0244 0.37 -0.3 298,300 - PP_0244 0.37 -1.4 298,521 + +0.1 298,522 - +0.2 298,532 + +0.5 298,532 + +0.1 298,533 - -1.2 298,541 + +0.5 298,541 + -0.4 298,541 + +0.9 298,542 - +1.4 298,833 + PP_0245 0.11 -1.4 298,834 - PP_0245 0.11 +0.1 298,834 - +0.3 298,888 - PP_0245 0.13 -0.3 299,022 - PP_0245 0.19 +0.3 299,462 + PP_0245 0.36 +0.3 299,462 + PP_0245 0.36 -0.4 299,463 - PP_0245 0.36 -0.4
Or see this region's nucleotide sequence