Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF960 and GFF961 are separated by 10 nucleotides GFF961 and GFF962 overlap by 4 nucleotides GFF962 and GFF963 overlap by 14 nucleotides
GFF960 - putative ATP-dependent protease, at 140,029 to 142,113
GFF960
GFF961 - putative cytoplasmic protein, at 142,124 to 144,727
GFF961
GFF962 - putative cytoplasmic protein, at 144,724 to 145,536
GFF962
GFF963 - putative ABC-type sugar/spermidine/putrescine transport system ATPase component, at 145,523 to 146,614
GFF963
Position (kb) 142
143
144
145 Strain fitness (log2 ratio) -2
-1
0
1
2 at 141.176 kb on + strand, within GFF960 at 141.176 kb on + strand, within GFF960 at 141.177 kb on - strand, within GFF960 at 141.356 kb on + strand, within GFF960 at 141.405 kb on - strand, within GFF960 at 141.494 kb on - strand, within GFF960 at 141.532 kb on - strand, within GFF960 at 141.545 kb on - strand, within GFF960 at 141.545 kb on - strand, within GFF960 at 141.561 kb on + strand, within GFF960 at 141.717 kb on + strand, within GFF960 at 141.717 kb on + strand, within GFF960 at 141.798 kb on - strand, within GFF960 at 141.854 kb on + strand, within GFF960 at 141.859 kb on - strand, within GFF960 at 141.931 kb on - strand at 141.953 kb on + strand at 142.366 kb on + strand at 142.366 kb on + strand at 142.547 kb on + strand, within GFF961 at 142.548 kb on - strand, within GFF961 at 142.723 kb on + strand, within GFF961 at 142.842 kb on - strand, within GFF961 at 142.844 kb on + strand, within GFF961 at 142.856 kb on + strand, within GFF961 at 142.882 kb on + strand, within GFF961 at 142.946 kb on - strand, within GFF961 at 142.968 kb on + strand, within GFF961 at 143.074 kb on + strand, within GFF961 at 143.132 kb on + strand, within GFF961 at 143.132 kb on + strand, within GFF961 at 143.133 kb on - strand, within GFF961 at 143.176 kb on - strand, within GFF961 at 143.183 kb on + strand, within GFF961 at 143.284 kb on + strand, within GFF961 at 143.412 kb on + strand, within GFF961 at 143.430 kb on - strand, within GFF961 at 143.469 kb on + strand, within GFF961 at 143.470 kb on - strand, within GFF961 at 143.542 kb on - strand, within GFF961 at 143.671 kb on - strand, within GFF961 at 143.675 kb on - strand, within GFF961 at 143.737 kb on + strand, within GFF961 at 143.925 kb on + strand, within GFF961 at 143.954 kb on - strand, within GFF961 at 144.257 kb on + strand, within GFF961 at 144.867 kb on + strand, within GFF962 at 144.919 kb on - strand, within GFF962 at 144.934 kb on + strand, within GFF962 at 144.935 kb on - strand, within GFF962 at 145.041 kb on + strand, within GFF962 at 145.042 kb on - strand, within GFF962 at 145.150 kb on + strand, within GFF962 at 145.252 kb on + strand, within GFF962 at 145.503 kb on + strand at 145.587 kb on + strand at 145.722 kb on - strand, within GFF963 at 145.722 kb on - strand, within GFF963
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 141,176 + GFF960 0.55 +0.1 141,176 + GFF960 0.55 +0.2 141,177 - GFF960 0.55 +0.8 141,356 + GFF960 0.64 -0.2 141,405 - GFF960 0.66 +0.6 141,494 - GFF960 0.70 -0.3 141,532 - GFF960 0.72 -2.6 141,545 - GFF960 0.73 +1.1 141,545 - GFF960 0.73 +0.4 141,561 + GFF960 0.73 +0.3 141,717 + GFF960 0.81 -0.1 141,717 + GFF960 0.81 -0.0 141,798 - GFF960 0.85 +0.4 141,854 + GFF960 0.88 +0.9 141,859 - GFF960 0.88 -0.5 141,931 - +1.3 141,953 + +0.7 142,366 + +0.4 142,366 + -0.3 142,547 + GFF961 0.16 +2.0 142,548 - GFF961 0.16 +0.6 142,723 + GFF961 0.23 +0.9 142,842 - GFF961 0.28 -0.1 142,844 + GFF961 0.28 +0.6 142,856 + GFF961 0.28 +0.1 142,882 + GFF961 0.29 +0.1 142,946 - GFF961 0.32 -0.2 142,968 + GFF961 0.32 +0.7 143,074 + GFF961 0.36 +0.5 143,132 + GFF961 0.39 +0.8 143,132 + GFF961 0.39 -0.0 143,133 - GFF961 0.39 +0.1 143,176 - GFF961 0.40 +0.6 143,183 + GFF961 0.41 +0.3 143,284 + GFF961 0.45 +0.5 143,412 + GFF961 0.49 -0.2 143,430 - GFF961 0.50 +0.8 143,469 + GFF961 0.52 +0.7 143,470 - GFF961 0.52 +0.3 143,542 - GFF961 0.54 +0.3 143,671 - GFF961 0.59 +0.5 143,675 - GFF961 0.60 -0.6 143,737 + GFF961 0.62 +0.5 143,925 + GFF961 0.69 +2.4 143,954 - GFF961 0.70 +0.9 144,257 + GFF961 0.82 +0.2 144,867 + GFF962 0.18 -0.2 144,919 - GFF962 0.24 +0.3 144,934 + GFF962 0.26 +0.8 144,935 - GFF962 0.26 +0.4 145,041 + GFF962 0.39 +0.4 145,042 - GFF962 0.39 +0.7 145,150 + GFF962 0.52 +0.3 145,252 + GFF962 0.65 +1.0 145,503 + +0.4 145,587 + -0.0 145,722 - GFF963 0.18 +1.4 145,722 - GFF963 0.18 +1.1
Or see this region's nucleotide sequence