Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF83

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF81 and GFF82 are separated by 91 nucleotidesGFF82 and GFF83 are separated by 138 nucleotidesGFF83 and GFF84 are separated by 140 nucleotides GFF81 - Ferric hydroxamate outer membrane receptor FhuA, at 63,162 to 65,273 GFF81 GFF82 - hypothetical protein, at 65,365 to 65,505 GFF82 GFF83 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 65,644 to 68,166 GFF83 GFF84 - ATP-dependent helicase HrpB, at 68,307 to 70,781 GFF84 Position (kb) 65 66 67 68 69Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 64.782 kb on - strand, within GFF81at 64.997 kb on + strand, within GFF81at 65.026 kb on - strand, within GFF81at 65.132 kb on + strandat 65.133 kb on - strandat 65.296 kb on - strandat 65.355 kb on + strandat 65.356 kb on - strandat 65.357 kb on + strandat 65.396 kb on - strand, within GFF82at 65.528 kb on + strandat 65.572 kb on + strandat 65.809 kb on - strandat 65.874 kb on - strandat 65.917 kb on + strand, within GFF83at 65.982 kb on - strand, within GFF83at 66.179 kb on + strand, within GFF83at 66.228 kb on - strand, within GFF83at 66.238 kb on - strand, within GFF83at 66.350 kb on - strand, within GFF83at 66.512 kb on + strand, within GFF83at 66.602 kb on + strand, within GFF83at 66.603 kb on - strand, within GFF83at 66.604 kb on + strand, within GFF83at 66.619 kb on + strand, within GFF83at 66.632 kb on + strand, within GFF83at 66.699 kb on - strand, within GFF83at 66.742 kb on - strand, within GFF83at 66.891 kb on + strand, within GFF83at 66.891 kb on + strand, within GFF83at 66.940 kb on - strand, within GFF83at 67.011 kb on - strand, within GFF83at 67.042 kb on + strand, within GFF83at 67.051 kb on + strand, within GFF83at 67.104 kb on - strand, within GFF83at 67.181 kb on + strand, within GFF83at 67.182 kb on - strand, within GFF83at 67.182 kb on - strand, within GFF83at 67.331 kb on + strand, within GFF83at 67.440 kb on + strand, within GFF83at 67.500 kb on + strand, within GFF83at 67.620 kb on + strand, within GFF83at 67.731 kb on + strand, within GFF83at 67.731 kb on + strand, within GFF83at 67.732 kb on - strand, within GFF83at 67.732 kb on - strand, within GFF83at 67.732 kb on - strand, within GFF83at 67.862 kb on + strand, within GFF83at 67.863 kb on - strand, within GFF83at 67.979 kb on - strandat 68.011 kb on - strandat 68.026 kb on - strandat 68.057 kb on + strandat 68.058 kb on - strandat 68.134 kb on - strandat 68.134 kb on - strandat 68.278 kb on + strandat 68.278 kb on + strandat 68.298 kb on + strandat 68.298 kb on + strandat 68.299 kb on - strandat 68.301 kb on - strandat 68.314 kb on + strandat 68.659 kb on + strand, within GFF84at 68.721 kb on + strand, within GFF84at 68.721 kb on + strand, within GFF84at 68.722 kb on - strand, within GFF84at 68.722 kb on - strand, within GFF84at 69.117 kb on - strand, within GFF84

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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64,782 - GFF81 0.77 +0.1
64,997 + GFF81 0.87 +0.6
65,026 - GFF81 0.88 +0.2
65,132 + +1.1
65,133 - +0.3
65,296 - -0.0
65,355 + -0.2
65,356 - +0.3
65,357 + +0.2
65,396 - GFF82 0.22 -0.4
65,528 + -0.1
65,572 + +0.3
65,809 - -1.4
65,874 - +0.2
65,917 + GFF83 0.11 +0.3
65,982 - GFF83 0.13 +0.3
66,179 + GFF83 0.21 -0.3
66,228 - GFF83 0.23 +2.2
66,238 - GFF83 0.24 +0.1
66,350 - GFF83 0.28 -0.8
66,512 + GFF83 0.34 +0.3
66,602 + GFF83 0.38 -0.2
66,603 - GFF83 0.38 +0.1
66,604 + GFF83 0.38 -0.9
66,619 + GFF83 0.39 -0.0
66,632 + GFF83 0.39 -1.3
66,699 - GFF83 0.42 -0.3
66,742 - GFF83 0.44 -0.5
66,891 + GFF83 0.49 +0.3
66,891 + GFF83 0.49 -0.1
66,940 - GFF83 0.51 +0.3
67,011 - GFF83 0.54 +0.3
67,042 + GFF83 0.55 +0.1
67,051 + GFF83 0.56 -0.4
67,104 - GFF83 0.58 +0.7
67,181 + GFF83 0.61 -1.6
67,182 - GFF83 0.61 -0.0
67,182 - GFF83 0.61 -1.4
67,331 + GFF83 0.67 -0.6
67,440 + GFF83 0.71 +0.7
67,500 + GFF83 0.74 +0.9
67,620 + GFF83 0.78 +0.0
67,731 + GFF83 0.83 +0.5
67,731 + GFF83 0.83 -0.7
67,732 - GFF83 0.83 -0.3
67,732 - GFF83 0.83 +0.3
67,732 - GFF83 0.83 +1.2
67,862 + GFF83 0.88 -1.2
67,863 - GFF83 0.88 +0.2
67,979 - +0.1
68,011 - -0.3
68,026 - +0.7
68,057 + -0.5
68,058 - +0.1
68,134 - -0.3
68,134 - +0.6
68,278 + +0.2
68,278 + +1.4
68,298 + +0.4
68,298 + +0.3
68,299 - +0.3
68,301 - +0.0
68,314 + +0.3
68,659 + GFF84 0.14 -3.1
68,721 + GFF84 0.17 -0.6
68,721 + GFF84 0.17 -1.8
68,722 - GFF84 0.17 -0.8
68,722 - GFF84 0.17 -0.1
69,117 - GFF84 0.33 +0.2

Or see this region's nucleotide sequence