Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF744 and GFF745 are separated by 44 nucleotides GFF745 and GFF746 are separated by 71 nucleotides GFF746 and GFF747 are separated by 312 nucleotides
GFF744 - D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4), at 102,693 to 103,763
GFF744
GFF745 - Deoxyribose operon repressor, DeoR family, at 103,808 to 104,566
GFF745
GFF746 - Putative permease, at 104,638 to 105,246
GFF746
GFF747 - Multidrug translocase MdfA, at 105,559 to 106,791
GFF747
Position (kb)
104
105
106 Strain fitness (log2 ratio)
-1
0
1
2 at 103.812 kb on + strand at 103.823 kb on - strand at 103.880 kb on - strand at 103.899 kb on - strand, within GFF745 at 103.923 kb on - strand, within GFF745 at 103.923 kb on - strand, within GFF745 at 104.021 kb on - strand, within GFF745 at 104.192 kb on - strand, within GFF745 at 104.195 kb on - strand, within GFF745 at 104.231 kb on - strand, within GFF745 at 104.542 kb on + strand at 104.566 kb on + strand at 104.583 kb on + strand at 104.617 kb on + strand at 104.617 kb on + strand at 104.618 kb on - strand at 104.788 kb on - strand, within GFF746 at 104.803 kb on + strand, within GFF746 at 104.804 kb on - strand, within GFF746 at 105.312 kb on + strand at 105.343 kb on - strand at 105.388 kb on + strand at 105.393 kb on + strand at 105.430 kb on + strand at 105.431 kb on - strand at 105.558 kb on + strand at 105.632 kb on - strand at 105.642 kb on + strand at 105.647 kb on - strand at 105.698 kb on - strand, within GFF747 at 105.754 kb on - strand, within GFF747 at 105.761 kb on + strand, within GFF747 at 105.883 kb on - strand, within GFF747 at 105.937 kb on + strand, within GFF747
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 103,812 + -0.1 103,823 - -0.2 103,880 - -0.7 103,899 - GFF745 0.12 +0.3 103,923 - GFF745 0.15 -0.4 103,923 - GFF745 0.15 -0.2 104,021 - GFF745 0.28 +0.3 104,192 - GFF745 0.51 -0.6 104,195 - GFF745 0.51 +1.4 104,231 - GFF745 0.56 +0.5 104,542 + -0.7 104,566 + -0.4 104,583 + -0.3 104,617 + -0.0 104,617 + +0.1 104,618 - +0.3 104,788 - GFF746 0.25 +1.1 104,803 + GFF746 0.27 -1.7 104,804 - GFF746 0.27 -0.8 105,312 + +0.6 105,343 - +1.9 105,388 + -1.3 105,393 + +0.1 105,430 + -0.7 105,431 - -0.6 105,558 + -0.4 105,632 - -0.0 105,642 + +1.6 105,647 - +0.5 105,698 - GFF747 0.11 -0.5 105,754 - GFF747 0.16 -0.1 105,761 + GFF747 0.16 -0.3 105,883 - GFF747 0.26 -1.2 105,937 + GFF747 0.31 +0.0
Or see this region's nucleotide sequence