Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF622

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF621 and GFF622 are separated by 289 nucleotidesGFF622 and GFF623 are separated by 95 nucleotides GFF621 - IgaA: a membrane protein that prevents overactivation of the Rcs regulatory system, at 360,763 to 362,895 GFF621 GFF622 - ADP compounds hydrolase NudE (EC 3.6.1.-), at 363,185 to 363,781 GFF622 GFF623 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 363,877 to 366,429 GFF623 Position (kb) 363 364Strain fitness (log2 ratio) -1 0 1at 362.910 kb on - strandat 362.913 kb on - strandat 363.032 kb on + strandat 363.033 kb on - strandat 363.039 kb on - strandat 363.174 kb on + strandat 363.232 kb on - strandat 363.340 kb on + strand, within GFF622at 363.341 kb on - strand, within GFF622at 363.341 kb on - strand, within GFF622at 363.403 kb on + strand, within GFF622at 363.660 kb on + strand, within GFF622at 363.904 kb on - strandat 364.092 kb on - strandat 364.238 kb on + strand, within GFF623at 364.359 kb on + strand, within GFF623at 364.568 kb on + strand, within GFF623at 364.569 kb on - strand, within GFF623at 364.661 kb on + strand, within GFF623at 364.662 kb on - strand, within GFF623at 364.768 kb on - strand, within GFF623

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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362,910 - +1.4
362,913 - -0.1
363,032 + -0.3
363,033 - -0.1
363,039 - +0.9
363,174 + +0.2
363,232 - +0.2
363,340 + GFF622 0.26 +0.1
363,341 - GFF622 0.26 -0.3
363,341 - GFF622 0.26 -1.0
363,403 + GFF622 0.37 -0.3
363,660 + GFF622 0.80 +0.0
363,904 - +0.3
364,092 - +0.2
364,238 + GFF623 0.14 -1.1
364,359 + GFF623 0.19 -0.6
364,568 + GFF623 0.27 -0.7
364,569 - GFF623 0.27 +1.3
364,661 + GFF623 0.31 +0.5
364,662 - GFF623 0.31 -0.6
364,768 - GFF623 0.35 -0.3

Or see this region's nucleotide sequence