Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF578 and GFF579 are separated by 19 nucleotides GFF579 and GFF580 are separated by 177 nucleotides GFF580 and GFF581 are separated by 43 nucleotides
GFF578 - Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21), at 310,266 to 311,699
GFF578
GFF579 - Glycogen phosphorylase (EC 2.4.1.1), at 311,719 to 314,166
GFF579
GFF580 - Transcriptional regulator, ArsR family, at 314,344 to 315,099
GFF580
GFF581 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 315,143 to 316,048
GFF581
Position (kb) 311
312
313
314
315 Strain fitness (log2 ratio) -1
0
1 at 310.758 kb on + strand, within GFF578 at 310.758 kb on + strand, within GFF578 at 310.817 kb on + strand, within GFF578 at 310.873 kb on - strand, within GFF578 at 310.875 kb on + strand, within GFF578 at 310.886 kb on + strand, within GFF578 at 310.886 kb on + strand, within GFF578 at 310.887 kb on - strand, within GFF578 at 310.888 kb on + strand, within GFF578 at 310.909 kb on - strand, within GFF578 at 310.909 kb on - strand, within GFF578 at 310.923 kb on - strand, within GFF578 at 310.996 kb on + strand, within GFF578 at 311.418 kb on + strand, within GFF578 at 311.418 kb on + strand, within GFF578 at 311.419 kb on - strand, within GFF578 at 311.419 kb on - strand, within GFF578 at 311.544 kb on - strand, within GFF578 at 311.617 kb on + strand at 311.617 kb on + strand at 311.646 kb on - strand at 311.686 kb on - strand at 311.697 kb on + strand at 311.719 kb on - strand at 311.733 kb on + strand at 311.734 kb on - strand at 311.734 kb on - strand at 311.779 kb on - strand at 311.779 kb on - strand at 311.785 kb on + strand at 311.788 kb on - strand at 311.899 kb on - strand at 311.936 kb on - strand at 311.968 kb on - strand, within GFF579 at 312.038 kb on + strand, within GFF579 at 312.175 kb on + strand, within GFF579 at 312.196 kb on - strand, within GFF579 at 312.472 kb on + strand, within GFF579 at 312.473 kb on - strand, within GFF579 at 312.561 kb on - strand, within GFF579 at 312.561 kb on - strand, within GFF579 at 312.577 kb on + strand, within GFF579 at 312.786 kb on + strand, within GFF579 at 312.786 kb on + strand, within GFF579 at 312.787 kb on - strand, within GFF579 at 312.795 kb on + strand, within GFF579 at 312.796 kb on - strand, within GFF579 at 312.870 kb on + strand, within GFF579 at 312.890 kb on - strand, within GFF579 at 313.342 kb on - strand, within GFF579 at 313.366 kb on - strand, within GFF579 at 313.371 kb on + strand, within GFF579 at 313.403 kb on + strand, within GFF579 at 313.504 kb on + strand, within GFF579 at 313.522 kb on - strand, within GFF579 at 313.590 kb on - strand, within GFF579 at 313.606 kb on + strand, within GFF579 at 313.771 kb on - strand, within GFF579 at 313.786 kb on - strand, within GFF579 at 313.804 kb on - strand, within GFF579 at 313.841 kb on - strand, within GFF579 at 313.856 kb on - strand, within GFF579 at 313.878 kb on - strand, within GFF579 at 313.886 kb on + strand, within GFF579 at 313.886 kb on + strand, within GFF579 at 313.915 kb on - strand, within GFF579 at 313.978 kb on + strand at 313.979 kb on - strand at 314.022 kb on + strand at 314.089 kb on - strand at 314.216 kb on + strand at 314.312 kb on - strand at 314.393 kb on + strand at 314.401 kb on + strand at 314.511 kb on - strand, within GFF580 at 314.531 kb on - strand, within GFF580 at 314.531 kb on - strand, within GFF580 at 314.531 kb on - strand, within GFF580 at 314.531 kb on - strand, within GFF580 at 314.531 kb on - strand, within GFF580 at 314.531 kb on - strand, within GFF580 at 314.562 kb on + strand, within GFF580 at 314.581 kb on + strand, within GFF580 at 314.582 kb on - strand, within GFF580 at 314.676 kb on + strand, within GFF580 at 314.677 kb on - strand, within GFF580 at 314.709 kb on + strand, within GFF580 at 314.747 kb on + strand, within GFF580 at 314.747 kb on + strand, within GFF580 at 314.748 kb on - strand, within GFF580 at 314.751 kb on + strand, within GFF580 at 314.751 kb on + strand, within GFF580 at 314.829 kb on - strand, within GFF580 at 314.915 kb on - strand, within GFF580 at 315.013 kb on - strand, within GFF580 at 315.051 kb on + strand at 315.076 kb on - strand at 315.162 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 310,758 + GFF578 0.34 -0.1 310,758 + GFF578 0.34 +0.4 310,817 + GFF578 0.38 +0.6 310,873 - GFF578 0.42 -0.8 310,875 + GFF578 0.42 +0.7 310,886 + GFF578 0.43 -0.9 310,886 + GFF578 0.43 +0.2 310,887 - GFF578 0.43 +0.0 310,888 + GFF578 0.43 -0.6 310,909 - GFF578 0.45 +0.5 310,909 - GFF578 0.45 +0.4 310,923 - GFF578 0.46 +0.2 310,996 + GFF578 0.51 -0.2 311,418 + GFF578 0.80 -0.4 311,418 + GFF578 0.80 +0.6 311,419 - GFF578 0.80 +0.2 311,419 - GFF578 0.80 +0.3 311,544 - GFF578 0.89 +0.2 311,617 + -0.2 311,617 + +0.3 311,646 - +0.5 311,686 - +0.1 311,697 + +1.7 311,719 - +0.7 311,733 + -0.0 311,734 - +0.2 311,734 - +0.6 311,779 - -0.7 311,779 - -0.9 311,785 + +0.9 311,788 - -0.1 311,899 - +0.5 311,936 - +0.2 311,968 - GFF579 0.10 +0.1 312,038 + GFF579 0.13 +0.1 312,175 + GFF579 0.19 +0.0 312,196 - GFF579 0.19 -0.4 312,472 + GFF579 0.31 +1.1 312,473 - GFF579 0.31 +0.5 312,561 - GFF579 0.34 +0.4 312,561 - GFF579 0.34 +0.4 312,577 + GFF579 0.35 +0.5 312,786 + GFF579 0.44 -1.7 312,786 + GFF579 0.44 +0.8 312,787 - GFF579 0.44 +1.5 312,795 + GFF579 0.44 +0.3 312,796 - GFF579 0.44 -0.0 312,870 + GFF579 0.47 +0.7 312,890 - GFF579 0.48 -0.4 313,342 - GFF579 0.66 +0.6 313,366 - GFF579 0.67 +0.1 313,371 + GFF579 0.67 +0.5 313,403 + GFF579 0.69 +0.2 313,504 + GFF579 0.73 +0.5 313,522 - GFF579 0.74 +0.3 313,590 - GFF579 0.76 -0.1 313,606 + GFF579 0.77 +0.2 313,771 - GFF579 0.84 +0.6 313,786 - GFF579 0.84 +0.5 313,804 - GFF579 0.85 +0.4 313,841 - GFF579 0.87 -0.1 313,856 - GFF579 0.87 -0.4 313,878 - GFF579 0.88 +0.4 313,886 + GFF579 0.89 +0.7 313,886 + GFF579 0.89 -0.6 313,915 - GFF579 0.90 +0.0 313,978 + +0.9 313,979 - -0.1 314,022 + +0.8 314,089 - +0.5 314,216 + -0.3 314,312 - +0.7 314,393 + +0.3 314,401 + +0.2 314,511 - GFF580 0.22 -0.5 314,531 - GFF580 0.25 +0.9 314,531 - GFF580 0.25 +0.4 314,531 - GFF580 0.25 +0.6 314,531 - GFF580 0.25 +0.3 314,531 - GFF580 0.25 -0.1 314,531 - GFF580 0.25 +0.0 314,562 + GFF580 0.29 -0.3 314,581 + GFF580 0.31 +0.1 314,582 - GFF580 0.31 +0.3 314,676 + GFF580 0.44 +0.2 314,677 - GFF580 0.44 +0.4 314,709 + GFF580 0.48 +0.7 314,747 + GFF580 0.53 -0.1 314,747 + GFF580 0.53 -0.6 314,748 - GFF580 0.53 +0.3 314,751 + GFF580 0.54 +0.6 314,751 + GFF580 0.54 +1.4 314,829 - GFF580 0.64 +0.2 314,915 - GFF580 0.76 +1.2 315,013 - GFF580 0.88 +0.4 315,051 + +1.0 315,076 - +0.2 315,162 - -0.1
Or see this region's nucleotide sequence