Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF491 and GFF492 are separated by 3 nucleotides GFF492 and GFF493 are separated by 71 nucleotides
GFF491 - Cyclic di-GMP binding protein precursor, at 212,247 to 214,547
GFF491
GFF492 - Endoglucanase precursor (EC 3.2.1.4), at 214,551 to 215,660
GFF492
GFF493 - Cellulose synthase operon protein C, at 215,732 to 219,184
GFF493
Position (kb) 214
215
216 Strain fitness (log2 ratio) -2
-1
0
1 at 213.652 kb on + strand, within GFF491 at 213.653 kb on - strand, within GFF491 at 213.653 kb on - strand, within GFF491 at 213.653 kb on - strand, within GFF491 at 213.669 kb on + strand, within GFF491 at 213.671 kb on + strand, within GFF491 at 213.672 kb on - strand, within GFF491 at 213.707 kb on + strand, within GFF491 at 213.707 kb on + strand, within GFF491 at 213.708 kb on - strand, within GFF491 at 213.708 kb on - strand, within GFF491 at 213.711 kb on - strand, within GFF491 at 213.775 kb on - strand, within GFF491 at 214.127 kb on + strand, within GFF491 at 214.277 kb on - strand, within GFF491 at 214.380 kb on + strand at 214.380 kb on + strand at 214.380 kb on + strand at 214.380 kb on + strand at 214.384 kb on - strand at 214.477 kb on + strand at 214.477 kb on + strand at 214.508 kb on + strand at 214.559 kb on + strand at 214.603 kb on + strand at 214.622 kb on + strand at 214.623 kb on - strand at 214.706 kb on + strand, within GFF492 at 214.729 kb on - strand, within GFF492 at 214.828 kb on - strand, within GFF492 at 215.076 kb on - strand, within GFF492 at 215.097 kb on + strand, within GFF492 at 215.098 kb on - strand, within GFF492 at 215.224 kb on - strand, within GFF492 at 215.278 kb on - strand, within GFF492 at 215.295 kb on + strand, within GFF492 at 215.345 kb on - strand, within GFF492 at 215.547 kb on + strand, within GFF492 at 215.547 kb on + strand, within GFF492 at 215.554 kb on - strand at 215.554 kb on - strand at 215.620 kb on + strand at 215.620 kb on + strand at 215.753 kb on - strand at 215.761 kb on - strand at 215.848 kb on - strand at 215.848 kb on - strand at 215.921 kb on + strand at 215.961 kb on + strand at 216.431 kb on + strand, within GFF493 at 216.485 kb on - strand, within GFF493
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 213,652 + GFF491 0.61 +0.4 213,653 - GFF491 0.61 -0.4 213,653 - GFF491 0.61 -0.3 213,653 - GFF491 0.61 -0.1 213,669 + GFF491 0.62 +0.6 213,671 + GFF491 0.62 +0.2 213,672 - GFF491 0.62 -0.1 213,707 + GFF491 0.63 -0.8 213,707 + GFF491 0.63 -0.1 213,708 - GFF491 0.63 -0.6 213,708 - GFF491 0.63 +0.5 213,711 - GFF491 0.64 +0.0 213,775 - GFF491 0.66 +0.6 214,127 + GFF491 0.82 -1.2 214,277 - GFF491 0.88 -0.1 214,380 + -0.4 214,380 + +1.4 214,380 + -0.1 214,380 + -0.4 214,384 - -0.0 214,477 + -0.3 214,477 + +0.6 214,508 + +0.3 214,559 + -1.2 214,603 + -1.9 214,622 + -0.4 214,623 - -0.7 214,706 + GFF492 0.14 -0.1 214,729 - GFF492 0.16 -0.5 214,828 - GFF492 0.25 -1.4 215,076 - GFF492 0.47 -0.7 215,097 + GFF492 0.49 -0.1 215,098 - GFF492 0.49 -0.3 215,224 - GFF492 0.61 -0.3 215,278 - GFF492 0.65 +0.4 215,295 + GFF492 0.67 -0.9 215,345 - GFF492 0.72 -0.1 215,547 + GFF492 0.90 -0.8 215,547 + GFF492 0.90 -0.1 215,554 - +0.0 215,554 - -0.8 215,620 + -0.4 215,620 + -0.3 215,753 - -0.3 215,761 - -0.2 215,848 - -0.3 215,848 - -1.7 215,921 + -0.6 215,961 + -0.2 216,431 + GFF493 0.20 -0.0 216,485 - GFF493 0.22 +0.9
Or see this region's nucleotide sequence