Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4764

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4763 and GFF4764 overlap by 4 nucleotidesGFF4764 and GFF4765 are separated by 140 nucleotides GFF4763 - Type III secretion outermembrane contact sensing protein (YopN,Yop4b,LcrE), at 70,265 to 71,383 GFF4763 GFF4764 - Type III secretion outermembrane pore forming protein (YscC,MxiD,HrcC, InvG), at 71,380 to 72,924 GFF4764 GFF4765 - Type III secretion thermoregulatory protein (LcrF,VirF,transcription regulation of virulence plasmid), at 73,065 to 73,814 GFF4765 Position (kb) 71 72 73Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 70.530 kb on - strand, within GFF4763at 70.601 kb on + strand, within GFF4763at 70.753 kb on - strand, within GFF4763at 70.753 kb on - strand, within GFF4763at 70.753 kb on - strand, within GFF4763at 70.829 kb on + strand, within GFF4763at 71.009 kb on + strand, within GFF4763at 71.010 kb on - strand, within GFF4763at 71.010 kb on - strand, within GFF4763at 71.076 kb on - strand, within GFF4763at 71.084 kb on + strand, within GFF4763at 71.302 kb on + strandat 71.302 kb on + strandat 71.302 kb on + strandat 71.302 kb on + strandat 71.431 kb on + strandat 71.566 kb on - strand, within GFF4764at 71.592 kb on + strand, within GFF4764at 71.704 kb on - strand, within GFF4764at 71.738 kb on - strand, within GFF4764at 71.748 kb on + strand, within GFF4764at 71.748 kb on + strand, within GFF4764at 71.748 kb on + strand, within GFF4764at 71.803 kb on + strand, within GFF4764at 71.804 kb on - strand, within GFF4764at 71.852 kb on + strand, within GFF4764at 71.852 kb on + strand, within GFF4764at 71.852 kb on + strand, within GFF4764at 71.857 kb on + strand, within GFF4764at 71.857 kb on + strand, within GFF4764at 71.857 kb on + strand, within GFF4764at 71.858 kb on - strand, within GFF4764at 71.877 kb on + strand, within GFF4764at 71.877 kb on + strand, within GFF4764at 71.877 kb on + strand, within GFF4764at 71.877 kb on + strand, within GFF4764at 71.878 kb on - strand, within GFF4764at 71.878 kb on - strand, within GFF4764at 71.878 kb on - strand, within GFF4764at 72.037 kb on + strand, within GFF4764at 72.078 kb on + strand, within GFF4764at 72.083 kb on - strand, within GFF4764at 72.083 kb on - strand, within GFF4764at 72.270 kb on + strand, within GFF4764at 72.271 kb on - strand, within GFF4764at 72.399 kb on + strand, within GFF4764at 72.400 kb on - strand, within GFF4764at 72.459 kb on + strand, within GFF4764at 72.507 kb on + strand, within GFF4764at 72.598 kb on - strand, within GFF4764at 72.627 kb on - strand, within GFF4764at 72.676 kb on + strand, within GFF4764at 72.765 kb on - strand, within GFF4764at 72.985 kb on + strandat 73.011 kb on + strandat 73.301 kb on - strand, within GFF4765at 73.362 kb on + strand, within GFF4765at 73.410 kb on + strand, within GFF4765at 73.416 kb on - strand, within GFF4765at 73.534 kb on + strand, within GFF4765at 73.600 kb on - strand, within GFF4765at 73.715 kb on + strand, within GFF4765at 73.716 kb on - strand, within GFF4765

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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70,530 - GFF4763 0.24 -1.3
70,601 + GFF4763 0.30 -0.5
70,753 - GFF4763 0.44 +0.1
70,753 - GFF4763 0.44 +1.3
70,753 - GFF4763 0.44 -1.9
70,829 + GFF4763 0.50 +0.4
71,009 + GFF4763 0.66 -0.7
71,010 - GFF4763 0.67 -0.9
71,010 - GFF4763 0.67 +0.7
71,076 - GFF4763 0.72 -0.3
71,084 + GFF4763 0.73 -0.4
71,302 + -2.3
71,302 + -0.7
71,302 + -1.0
71,302 + -0.3
71,431 + +0.0
71,566 - GFF4764 0.12 -0.6
71,592 + GFF4764 0.14 -0.2
71,704 - GFF4764 0.21 +0.5
71,738 - GFF4764 0.23 -1.8
71,748 + GFF4764 0.24 -0.6
71,748 + GFF4764 0.24 -1.2
71,748 + GFF4764 0.24 -0.7
71,803 + GFF4764 0.27 -0.2
71,804 - GFF4764 0.27 +1.6
71,852 + GFF4764 0.31 -0.4
71,852 + GFF4764 0.31 -1.0
71,852 + GFF4764 0.31 -2.0
71,857 + GFF4764 0.31 +0.8
71,857 + GFF4764 0.31 -2.8
71,857 + GFF4764 0.31 -0.2
71,858 - GFF4764 0.31 -0.3
71,877 + GFF4764 0.32 -1.2
71,877 + GFF4764 0.32 +0.2
71,877 + GFF4764 0.32 +0.2
71,877 + GFF4764 0.32 +0.5
71,878 - GFF4764 0.32 +0.6
71,878 - GFF4764 0.32 -0.1
71,878 - GFF4764 0.32 +0.3
72,037 + GFF4764 0.43 -0.6
72,078 + GFF4764 0.45 +0.1
72,083 - GFF4764 0.46 +0.1
72,083 - GFF4764 0.46 +1.2
72,270 + GFF4764 0.58 +0.0
72,271 - GFF4764 0.58 +0.4
72,399 + GFF4764 0.66 +0.6
72,400 - GFF4764 0.66 -0.3
72,459 + GFF4764 0.70 -0.4
72,507 + GFF4764 0.73 +0.4
72,598 - GFF4764 0.79 -1.0
72,627 - GFF4764 0.81 -0.4
72,676 + GFF4764 0.84 -0.3
72,765 - GFF4764 0.90 +0.3
72,985 + -0.6
73,011 + +0.2
73,301 - GFF4765 0.31 -0.9
73,362 + GFF4765 0.40 +0.2
73,410 + GFF4765 0.46 +0.1
73,416 - GFF4765 0.47 -0.2
73,534 + GFF4765 0.63 +0.1
73,600 - GFF4765 0.71 +0.1
73,715 + GFF4765 0.87 -0.9
73,716 - GFF4765 0.87 +1.9

Or see this region's nucleotide sequence