Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4671 and GFF4672 overlap by 4 nucleotides GFF4672 and GFF4673 overlap by 4 nucleotides GFF4673 and GFF4674 overlap by 50 nucleotides
GFF4671 - Phage terminase, large subunit, at 1,276 to 3,309
GFF4671
GFF4672 - FIG01047026: hypothetical protein, at 3,306 to 3,512
GFF4672
GFF4673 - Phage portal protein, at 3,509 to 5,056
GFF4673
GFF4674 - Prophage Clp protease-like protein, at 5,007 to 7,061
GFF4674
Position (kb) 3
4
5
6 Strain fitness (log2 ratio) -1
0
1 at 2.834 kb on - strand, within GFF4671 at 2.893 kb on + strand, within GFF4671 at 2.893 kb on + strand, within GFF4671 at 2.900 kb on + strand, within GFF4671 at 2.965 kb on + strand, within GFF4671 at 2.966 kb on - strand, within GFF4671 at 3.016 kb on + strand, within GFF4671 at 3.091 kb on + strand, within GFF4671 at 3.333 kb on + strand, within GFF4672 at 3.390 kb on - strand, within GFF4672 at 3.585 kb on + strand at 3.707 kb on - strand, within GFF4673 at 3.888 kb on - strand, within GFF4673 at 3.890 kb on - strand, within GFF4673 at 3.890 kb on - strand, within GFF4673 at 3.890 kb on - strand, within GFF4673 at 4.064 kb on - strand, within GFF4673 at 4.082 kb on + strand, within GFF4673 at 4.082 kb on + strand, within GFF4673 at 4.278 kb on - strand, within GFF4673 at 4.376 kb on + strand, within GFF4673 at 4.530 kb on + strand, within GFF4673 at 4.549 kb on + strand, within GFF4673 at 4.563 kb on - strand, within GFF4673 at 4.627 kb on - strand, within GFF4673 at 4.810 kb on - strand, within GFF4673 at 4.916 kb on - strand at 5.087 kb on + strand at 5.221 kb on + strand, within GFF4674 at 5.280 kb on + strand, within GFF4674 at 5.326 kb on + strand, within GFF4674 at 5.330 kb on + strand, within GFF4674 at 5.343 kb on - strand, within GFF4674 at 5.373 kb on - strand, within GFF4674 at 5.464 kb on - strand, within GFF4674 at 5.573 kb on - strand, within GFF4674 at 5.651 kb on + strand, within GFF4674 at 5.752 kb on + strand, within GFF4674 at 5.752 kb on + strand, within GFF4674 at 5.795 kb on + strand, within GFF4674 at 5.802 kb on - strand, within GFF4674 at 5.802 kb on - strand, within GFF4674 at 5.994 kb on + strand, within GFF4674 at 5.995 kb on - strand, within GFF4674
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 2,834 - GFF4671 0.77 +0.4 2,893 + GFF4671 0.79 -0.2 2,893 + GFF4671 0.79 -0.3 2,900 + GFF4671 0.80 +0.1 2,965 + GFF4671 0.83 -0.2 2,966 - GFF4671 0.83 -0.9 3,016 + GFF4671 0.86 -0.7 3,091 + GFF4671 0.89 -0.1 3,333 + GFF4672 0.13 -0.8 3,390 - GFF4672 0.41 +0.7 3,585 + +0.5 3,707 - GFF4673 0.13 -0.7 3,888 - GFF4673 0.24 -0.1 3,890 - GFF4673 0.25 +0.8 3,890 - GFF4673 0.25 -0.3 3,890 - GFF4673 0.25 +0.4 4,064 - GFF4673 0.36 -0.5 4,082 + GFF4673 0.37 -0.1 4,082 + GFF4673 0.37 -0.3 4,278 - GFF4673 0.50 -0.7 4,376 + GFF4673 0.56 -1.4 4,530 + GFF4673 0.66 -0.3 4,549 + GFF4673 0.67 -0.3 4,563 - GFF4673 0.68 +0.0 4,627 - GFF4673 0.72 +0.9 4,810 - GFF4673 0.84 -0.2 4,916 - +0.2 5,087 + -0.5 5,221 + GFF4674 0.10 +0.5 5,280 + GFF4674 0.13 +0.6 5,326 + GFF4674 0.16 -0.9 5,330 + GFF4674 0.16 -0.5 5,343 - GFF4674 0.16 +0.8 5,373 - GFF4674 0.18 +0.3 5,464 - GFF4674 0.22 +1.0 5,573 - GFF4674 0.28 +0.5 5,651 + GFF4674 0.31 +0.1 5,752 + GFF4674 0.36 +0.7 5,752 + GFF4674 0.36 -0.9 5,795 + GFF4674 0.38 +0.2 5,802 - GFF4674 0.39 -0.2 5,802 - GFF4674 0.39 +0.6 5,994 + GFF4674 0.48 +0.2 5,995 - GFF4674 0.48 -0.8
Or see this region's nucleotide sequence