Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4472 and GFF4473 are separated by 26 nucleotides GFF4473 and GFF4474 are separated by 98 nucleotides GFF4474 and GFF4475 are separated by 253 nucleotides
GFF4472 - Uncharacterized protein ybdG, at 255,849 to 257,096
GFF4472
GFF4473 - FIG01046818: hypothetical protein, at 257,123 to 257,308
GFF4473
GFF4474 - Outer membrane esterase, at 257,407 to 259,377
GFF4474
GFF4475 - NtrC family Transcriptional regulator, ATPase domain, at 259,631 to 262,273
GFF4475
Position (kb) 257
258
259
260 Strain fitness (log2 ratio) -1
0
1
2 at 256.433 kb on + strand, within GFF4472 at 256.459 kb on + strand, within GFF4472 at 256.894 kb on - strand, within GFF4472 at 256.899 kb on - strand, within GFF4472 at 256.965 kb on + strand, within GFF4472 at 256.968 kb on + strand, within GFF4472 at 257.145 kb on + strand, within GFF4473 at 257.224 kb on - strand, within GFF4473 at 257.344 kb on + strand at 257.375 kb on + strand at 257.379 kb on + strand at 257.394 kb on + strand at 257.493 kb on + strand at 257.494 kb on - strand at 257.515 kb on - strand at 257.529 kb on + strand at 257.749 kb on + strand, within GFF4474 at 257.749 kb on + strand, within GFF4474 at 257.750 kb on - strand, within GFF4474 at 257.750 kb on - strand, within GFF4474 at 257.901 kb on - strand, within GFF4474 at 258.131 kb on - strand, within GFF4474 at 258.219 kb on - strand, within GFF4474 at 258.247 kb on - strand, within GFF4474 at 258.288 kb on + strand, within GFF4474 at 258.288 kb on + strand, within GFF4474 at 258.288 kb on + strand, within GFF4474 at 258.412 kb on - strand, within GFF4474 at 258.418 kb on - strand, within GFF4474 at 258.430 kb on - strand, within GFF4474 at 258.508 kb on - strand, within GFF4474 at 258.610 kb on - strand, within GFF4474 at 258.612 kb on + strand, within GFF4474 at 258.739 kb on + strand, within GFF4474 at 258.740 kb on - strand, within GFF4474 at 258.860 kb on + strand, within GFF4474 at 258.985 kb on + strand, within GFF4474 at 259.038 kb on + strand, within GFF4474 at 259.039 kb on - strand, within GFF4474 at 259.039 kb on - strand, within GFF4474 at 259.141 kb on - strand, within GFF4474 at 259.167 kb on + strand, within GFF4474 at 259.167 kb on + strand, within GFF4474 at 259.167 kb on + strand, within GFF4474 at 259.171 kb on + strand, within GFF4474 at 259.171 kb on + strand, within GFF4474 at 259.171 kb on + strand, within GFF4474 at 259.172 kb on - strand, within GFF4474 at 259.191 kb on + strand at 259.350 kb on + strand at 259.351 kb on - strand at 259.672 kb on + strand at 259.673 kb on - strand at 259.684 kb on + strand at 259.892 kb on - strand at 259.892 kb on - strand at 259.908 kb on - strand, within GFF4475 at 260.060 kb on - strand, within GFF4475 at 260.284 kb on - strand, within GFF4475 at 260.339 kb on - strand, within GFF4475
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 256,433 + GFF4472 0.47 +0.0 256,459 + GFF4472 0.49 +0.6 256,894 - GFF4472 0.84 +0.1 256,899 - GFF4472 0.84 +0.3 256,965 + GFF4472 0.89 +0.1 256,968 + GFF4472 0.90 +0.3 257,145 + GFF4473 0.12 -1.5 257,224 - GFF4473 0.54 -0.5 257,344 + +0.4 257,375 + -0.0 257,379 + -0.4 257,394 + -0.3 257,493 + +0.1 257,494 - +0.2 257,515 - -0.3 257,529 + +0.1 257,749 + GFF4474 0.17 +0.3 257,749 + GFF4474 0.17 -0.2 257,750 - GFF4474 0.17 +0.6 257,750 - GFF4474 0.17 +0.3 257,901 - GFF4474 0.25 +0.2 258,131 - GFF4474 0.37 -0.4 258,219 - GFF4474 0.41 -0.1 258,247 - GFF4474 0.43 -0.1 258,288 + GFF4474 0.45 -0.4 258,288 + GFF4474 0.45 +0.5 258,288 + GFF4474 0.45 -1.2 258,412 - GFF4474 0.51 +0.5 258,418 - GFF4474 0.51 +0.5 258,430 - GFF4474 0.52 +2.2 258,508 - GFF4474 0.56 -0.5 258,610 - GFF4474 0.61 -1.5 258,612 + GFF4474 0.61 -1.5 258,739 + GFF4474 0.68 +0.5 258,740 - GFF4474 0.68 +1.0 258,860 + GFF4474 0.74 +0.5 258,985 + GFF4474 0.80 +0.5 259,038 + GFF4474 0.83 -0.2 259,039 - GFF4474 0.83 -0.1 259,039 - GFF4474 0.83 +0.3 259,141 - GFF4474 0.88 +0.4 259,167 + GFF4474 0.89 +0.1 259,167 + GFF4474 0.89 +0.3 259,167 + GFF4474 0.89 +0.3 259,171 + GFF4474 0.89 +0.5 259,171 + GFF4474 0.89 +0.1 259,171 + GFF4474 0.89 -0.5 259,172 - GFF4474 0.90 -0.3 259,191 + +0.3 259,350 + +0.8 259,351 - -0.2 259,672 + +0.6 259,673 - -0.0 259,684 + -0.5 259,892 - +0.3 259,892 - -0.4 259,908 - GFF4475 0.10 +0.7 260,060 - GFF4475 0.16 -0.4 260,284 - GFF4475 0.25 -0.8 260,339 - GFF4475 0.27 -0.6
Or see this region's nucleotide sequence