Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF441 and GFF442 are separated by 60 nucleotides GFF442 and GFF443 are separated by 104 nucleotides GFF443 and GFF444 are separated by 81 nucleotides
GFF441 - BAX protein, at 163,115 to 163,687
GFF441
GFF442 - Xylose activator XylR (AraC family), at 163,748 to 164,926
GFF442
GFF443 - FIG01045230: hypothetical protein, at 165,031 to 165,207
GFF443
GFF444 - Xylose isomerase (EC 5.3.1.5), at 165,289 to 166,611
GFF444
Position (kb) 163
164
165 Strain fitness (log2 ratio) -1
0
1
2 at 162.772 kb on + strand at 162.867 kb on + strand at 162.904 kb on + strand at 162.904 kb on + strand at 162.905 kb on - strand at 162.910 kb on - strand at 162.981 kb on + strand at 162.995 kb on + strand at 163.012 kb on + strand at 163.022 kb on + strand at 163.023 kb on - strand at 163.122 kb on - strand at 163.148 kb on - strand at 163.181 kb on + strand, within GFF441 at 163.270 kb on + strand, within GFF441 at 163.270 kb on + strand, within GFF441 at 163.283 kb on - strand, within GFF441 at 163.375 kb on + strand, within GFF441 at 163.376 kb on - strand, within GFF441 at 163.421 kb on - strand, within GFF441 at 163.488 kb on - strand, within GFF441 at 163.518 kb on - strand, within GFF441 at 163.591 kb on + strand, within GFF441 at 163.604 kb on + strand, within GFF441 at 163.628 kb on - strand, within GFF441 at 163.628 kb on - strand, within GFF441 at 163.634 kb on + strand at 163.634 kb on + strand at 163.634 kb on + strand at 163.635 kb on - strand at 163.649 kb on + strand at 163.685 kb on + strand at 163.685 kb on + strand at 163.686 kb on - strand at 163.709 kb on - strand at 163.722 kb on + strand at 163.735 kb on + strand at 163.735 kb on + strand at 163.749 kb on + strand at 163.749 kb on + strand at 163.749 kb on + strand at 163.765 kb on + strand at 163.801 kb on - strand at 163.827 kb on + strand at 163.828 kb on - strand at 163.937 kb on + strand, within GFF442 at 164.144 kb on + strand, within GFF442 at 164.250 kb on + strand, within GFF442 at 164.251 kb on - strand, within GFF442 at 164.259 kb on - strand, within GFF442 at 164.270 kb on - strand, within GFF442 at 164.462 kb on + strand, within GFF442 at 164.554 kb on - strand, within GFF442 at 164.895 kb on - strand at 164.933 kb on - strand at 164.985 kb on + strand at 165.084 kb on - strand, within GFF443 at 165.210 kb on + strand at 165.210 kb on + strand at 165.211 kb on - strand at 165.221 kb on + strand at 165.256 kb on + strand at 165.288 kb on + strand at 165.399 kb on + strand at 165.439 kb on - strand, within GFF444 at 165.463 kb on - strand, within GFF444 at 165.473 kb on + strand, within GFF444 at 165.473 kb on + strand, within GFF444 at 165.631 kb on + strand, within GFF444 at 165.631 kb on + strand, within GFF444 at 165.633 kb on + strand, within GFF444 at 165.634 kb on - strand, within GFF444 at 165.692 kb on + strand, within GFF444 at 165.822 kb on + strand, within GFF444 at 165.822 kb on + strand, within GFF444
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 162,772 + +0.3 162,867 + +0.0 162,904 + +0.2 162,904 + -1.0 162,905 - +0.1 162,910 - +0.3 162,981 + +0.3 162,995 + +0.3 163,012 + -0.5 163,022 + -0.8 163,023 - -0.5 163,122 - -0.4 163,148 - +0.1 163,181 + GFF441 0.12 -0.3 163,270 + GFF441 0.27 +1.9 163,270 + GFF441 0.27 -0.1 163,283 - GFF441 0.29 +0.3 163,375 + GFF441 0.45 +0.5 163,376 - GFF441 0.46 -0.1 163,421 - GFF441 0.53 +0.4 163,488 - GFF441 0.65 -0.2 163,518 - GFF441 0.70 -0.4 163,591 + GFF441 0.83 -0.9 163,604 + GFF441 0.85 +2.3 163,628 - GFF441 0.90 +0.1 163,628 - GFF441 0.90 -0.9 163,634 + -0.1 163,634 + -0.4 163,634 + +0.4 163,635 - -0.0 163,649 + +1.0 163,685 + +0.3 163,685 + +0.0 163,686 - +0.2 163,709 - -0.3 163,722 + +0.7 163,735 + -0.9 163,735 + -0.4 163,749 + -0.3 163,749 + -0.2 163,749 + +0.4 163,765 + -0.2 163,801 - -0.9 163,827 + +0.1 163,828 - +0.1 163,937 + GFF442 0.16 +0.2 164,144 + GFF442 0.34 +0.6 164,250 + GFF442 0.43 +0.1 164,251 - GFF442 0.43 +0.2 164,259 - GFF442 0.43 -0.3 164,270 - GFF442 0.44 +0.3 164,462 + GFF442 0.61 -0.4 164,554 - GFF442 0.68 +0.6 164,895 - -0.2 164,933 - +0.2 164,985 + -0.3 165,084 - GFF443 0.30 -0.2 165,210 + +0.3 165,210 + +0.1 165,211 - +0.4 165,221 + -0.0 165,256 + +0.4 165,288 + +0.6 165,399 + +0.0 165,439 - GFF444 0.11 -0.9 165,463 - GFF444 0.13 +0.5 165,473 + GFF444 0.14 -0.0 165,473 + GFF444 0.14 +0.4 165,631 + GFF444 0.26 -1.2 165,631 + GFF444 0.26 -0.3 165,633 + GFF444 0.26 +0.5 165,634 - GFF444 0.26 +0.3 165,692 + GFF444 0.30 -0.3 165,822 + GFF444 0.40 -0.6 165,822 + GFF444 0.40 +0.1
Or see this region's nucleotide sequence