Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4309 and GFF4310 are separated by 25 nucleotides GFF4310 and GFF4311 are separated by 169 nucleotides GFF4311 and GFF4312 are separated by 392 nucleotides GFF4312 and GFF4313 are separated by 83 nucleotides
GFF4309 - Glyoxalase family protein, at 95,520 to 95,885
GFF4309
GFF4310 - Transcriptional regulator, DeoR family, at 95,911 to 96,603
GFF4310
GFF4311 - hypothetical protein, at 96,773 to 97,120
GFF4311
GFF4312 - hypothetical protein, at 97,513 to 97,665
GFF4312
GFF4313 - Nucleoside-specific channel-forming protein Tsx precursor, at 97,749 to 98,612
GFF4313
Position (kb)
96
97
98 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 95.841 kb on - strand, within GFF4309 at 95.864 kb on - strand at 95.869 kb on + strand at 95.869 kb on + strand at 95.870 kb on - strand at 95.887 kb on - strand at 95.893 kb on + strand at 96.127 kb on - strand, within GFF4310 at 96.127 kb on - strand, within GFF4310 at 96.159 kb on + strand, within GFF4310 at 96.168 kb on - strand, within GFF4310 at 96.280 kb on + strand, within GFF4310 at 96.331 kb on - strand, within GFF4310 at 96.410 kb on + strand, within GFF4310 at 96.411 kb on - strand, within GFF4310 at 96.411 kb on - strand, within GFF4310 at 96.411 kb on - strand, within GFF4310 at 96.677 kb on + strand at 96.687 kb on + strand at 96.687 kb on + strand at 96.689 kb on + strand at 96.689 kb on + strand at 96.689 kb on + strand at 96.690 kb on - strand at 96.690 kb on - strand at 96.769 kb on - strand at 96.772 kb on + strand at 96.961 kb on - strand, within GFF4311 at 96.994 kb on + strand, within GFF4311 at 96.995 kb on - strand, within GFF4311 at 97.074 kb on - strand, within GFF4311 at 97.074 kb on - strand, within GFF4311 at 97.205 kb on + strand at 97.288 kb on - strand at 97.582 kb on - strand, within GFF4312 at 97.584 kb on + strand, within GFF4312 at 97.585 kb on - strand, within GFF4312 at 97.960 kb on - strand, within GFF4313 at 97.997 kb on - strand, within GFF4313 at 98.104 kb on + strand, within GFF4313 at 98.113 kb on + strand, within GFF4313 at 98.114 kb on - strand, within GFF4313
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 95,841 - GFF4309 0.88 +1.5 95,864 - -0.3 95,869 + -0.4 95,869 + +0.0 95,870 - -1.1 95,887 - -1.0 95,893 + +0.1 96,127 - GFF4310 0.31 -0.4 96,127 - GFF4310 0.31 -0.2 96,159 + GFF4310 0.36 -0.5 96,168 - GFF4310 0.37 +1.3 96,280 + GFF4310 0.53 -0.3 96,331 - GFF4310 0.61 +0.4 96,410 + GFF4310 0.72 +0.8 96,411 - GFF4310 0.72 +1.1 96,411 - GFF4310 0.72 -2.7 96,411 - GFF4310 0.72 -0.4 96,677 + -0.0 96,687 + +0.2 96,687 + -0.5 96,689 + -0.5 96,689 + -0.2 96,689 + +0.1 96,690 - -0.3 96,690 - -0.5 96,769 - +0.3 96,772 + +0.3 96,961 - GFF4311 0.54 +0.1 96,994 + GFF4311 0.64 -1.2 96,995 - GFF4311 0.64 -0.4 97,074 - GFF4311 0.86 -1.8 97,074 - GFF4311 0.86 -1.1 97,205 + -0.3 97,288 - -0.9 97,582 - GFF4312 0.45 +0.5 97,584 + GFF4312 0.46 +0.5 97,585 - GFF4312 0.47 -1.8 97,960 - GFF4313 0.24 -0.8 97,997 - GFF4313 0.29 +2.7 98,104 + GFF4313 0.41 -0.7 98,113 + GFF4313 0.42 +0.3 98,114 - GFF4313 0.42 -0.1
Or see this region's nucleotide sequence