Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4269 and GFF4270 are separated by 85 nucleotides GFF4270 and GFF4271 are separated by 44 nucleotides GFF4271 and GFF4272 are separated by 346 nucleotides
GFF4269 - Putative flagellin structural protein, at 55,518 to 58,313
GFF4269
GFF4270 - FIG00637869: hypothetical protein, at 58,399 to 59,022
GFF4270
GFF4271 - Penicillin-binding protein AmpH, at 59,067 to 60,224
GFF4271
GFF4272 - SbmA protein, at 60,571 to 61,791
GFF4272
Position (kb) 59
60
61 Strain fitness (log2 ratio) -1
0
1
2 at 58.141 kb on + strand at 58.168 kb on + strand at 58.249 kb on - strand at 58.299 kb on - strand at 58.304 kb on + strand at 58.398 kb on + strand at 58.399 kb on - strand at 58.519 kb on - strand, within GFF4270 at 59.048 kb on + strand at 59.139 kb on - strand at 59.192 kb on + strand, within GFF4271 at 59.215 kb on + strand, within GFF4271 at 59.283 kb on - strand, within GFF4271 at 59.284 kb on + strand, within GFF4271 at 59.284 kb on + strand, within GFF4271 at 59.396 kb on + strand, within GFF4271 at 59.423 kb on - strand, within GFF4271 at 59.462 kb on - strand, within GFF4271 at 59.462 kb on - strand, within GFF4271 at 59.492 kb on + strand, within GFF4271 at 59.499 kb on - strand, within GFF4271 at 59.615 kb on + strand, within GFF4271 at 59.615 kb on + strand, within GFF4271 at 59.616 kb on - strand, within GFF4271 at 59.852 kb on - strand, within GFF4271 at 59.882 kb on - strand, within GFF4271 at 60.094 kb on + strand, within GFF4271 at 60.095 kb on - strand, within GFF4271 at 60.095 kb on - strand, within GFF4271 at 60.102 kb on - strand, within GFF4271 at 60.176 kb on + strand at 60.215 kb on + strand at 60.237 kb on + strand at 60.237 kb on + strand at 60.237 kb on + strand at 60.238 kb on - strand at 60.247 kb on - strand at 60.247 kb on - strand at 60.267 kb on - strand at 60.267 kb on - strand at 60.268 kb on + strand at 60.388 kb on - strand at 60.452 kb on + strand at 60.467 kb on - strand at 60.467 kb on - strand at 60.467 kb on - strand at 60.516 kb on + strand at 60.758 kb on - strand, within GFF4272 at 60.802 kb on + strand, within GFF4272 at 60.802 kb on + strand, within GFF4272 at 61.054 kb on + strand, within GFF4272 at 61.054 kb on + strand, within GFF4272 at 61.055 kb on - strand, within GFF4272 at 61.058 kb on + strand, within GFF4272 at 61.059 kb on - strand, within GFF4272 at 61.059 kb on - strand, within GFF4272 at 61.090 kb on + strand, within GFF4272 at 61.090 kb on + strand, within GFF4272 at 61.091 kb on - strand, within GFF4272 at 61.092 kb on + strand, within GFF4272 at 61.092 kb on + strand, within GFF4272 at 61.092 kb on + strand, within GFF4272 at 61.108 kb on + strand, within GFF4272 at 61.152 kb on + strand, within GFF4272 at 61.153 kb on - strand, within GFF4272
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 58,141 + +0.1 58,168 + -0.4 58,249 - -0.1 58,299 - -0.5 58,304 + -0.6 58,398 + -0.2 58,399 - -0.1 58,519 - GFF4270 0.19 +0.6 59,048 + +0.1 59,139 - -0.5 59,192 + GFF4271 0.11 -0.2 59,215 + GFF4271 0.13 -0.0 59,283 - GFF4271 0.19 +0.8 59,284 + GFF4271 0.19 +0.7 59,284 + GFF4271 0.19 -0.2 59,396 + GFF4271 0.28 -0.0 59,423 - GFF4271 0.31 +1.1 59,462 - GFF4271 0.34 -1.3 59,462 - GFF4271 0.34 -0.9 59,492 + GFF4271 0.37 -0.1 59,499 - GFF4271 0.37 -1.1 59,615 + GFF4271 0.47 +1.9 59,615 + GFF4271 0.47 +0.2 59,616 - GFF4271 0.47 -1.6 59,852 - GFF4271 0.68 -0.7 59,882 - GFF4271 0.70 +0.9 60,094 + GFF4271 0.89 +0.2 60,095 - GFF4271 0.89 -0.5 60,095 - GFF4271 0.89 -0.2 60,102 - GFF4271 0.89 -0.1 60,176 + -1.0 60,215 + -0.7 60,237 + -0.1 60,237 + -0.2 60,237 + +0.1 60,238 - -0.0 60,247 - +0.2 60,247 - -0.2 60,267 - -0.3 60,267 - -1.1 60,268 + -0.2 60,388 - +0.5 60,452 + +0.1 60,467 - -0.1 60,467 - +1.5 60,467 - -0.2 60,516 + -1.1 60,758 - GFF4272 0.15 -1.2 60,802 + GFF4272 0.19 -0.5 60,802 + GFF4272 0.19 +0.1 61,054 + GFF4272 0.40 +0.1 61,054 + GFF4272 0.40 -1.2 61,055 - GFF4272 0.40 +0.4 61,058 + GFF4272 0.40 -0.1 61,059 - GFF4272 0.40 -1.1 61,059 - GFF4272 0.40 -0.2 61,090 + GFF4272 0.43 +0.3 61,090 + GFF4272 0.43 +0.1 61,091 - GFF4272 0.43 +0.7 61,092 + GFF4272 0.43 -0.0 61,092 + GFF4272 0.43 +0.0 61,092 + GFF4272 0.43 +0.6 61,108 + GFF4272 0.44 -0.5 61,152 + GFF4272 0.48 -0.0 61,153 - GFF4272 0.48 -0.0
Or see this region's nucleotide sequence