Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4140

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4138 and GFF4139 overlap by 4 nucleotidesGFF4139 and GFF4140 are separated by 112 nucleotidesGFF4140 and GFF4141 are separated by 221 nucleotidesGFF4141 and GFF4142 are separated by 83 nucleotidesGFF4142 and GFF4143 overlap by 24 nucleotides GFF4138 - PTS system, glucitol/sorbitol-specific IIA component (EC 2.7.1.69), at 21,076 to 21,450 GFF4138 GFF4139 - PTS system, glucitol/sorbitol-specific IIC component (EC 2.7.1.69), at 21,447 to 21,968 GFF4139 GFF4140 - putative cytoplasmic protein, at 22,081 to 22,365 GFF4140 GFF4141 - FIG01047227: hypothetical protein, at 22,587 to 22,748 GFF4141 GFF4142 - FIG01047293: hypothetical protein, at 22,832 to 22,993 GFF4142 GFF4143 - putative cytoplasmic protein, at 22,970 to 23,836 GFF4143 Position (kb) 22 23Strain fitness (log2 ratio) -1 0 1at 21.255 kb on - strand, within GFF4138at 21.429 kb on + strandat 21.488 kb on - strandat 21.519 kb on + strand, within GFF4139at 21.520 kb on - strand, within GFF4139at 21.703 kb on - strand, within GFF4139at 22.083 kb on - strandat 22.158 kb on - strand, within GFF4140at 22.492 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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21,255 - GFF4138 0.48 +0.2
21,429 + +0.8
21,488 - -0.4
21,519 + GFF4139 0.14 -0.4
21,520 - GFF4139 0.14 +0.6
21,703 - GFF4139 0.49 -0.4
22,083 - -0.1
22,158 - GFF4140 0.27 -0.7
22,492 + +0.4

Or see this region's nucleotide sequence