Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4116 and GFF4038 are separated by 164 nucleotides GFF4038 and GFF4039 are separated by 20 nucleotides GFF4039 and GFF4040 are separated by 104 nucleotides
GFF4116 - tRNA-Asp-GTC, at 238 to 314
GFF4116
GFF4038 - Methylglyoxal reductase, acetol producing (EC 1.1.1.-) / 2,5-diketo-D-gluconic acid reductase B (EC 1.1.1.274), at 479 to 1,282
GFF4038
GFF4039 - LysR family transcriptional regulator YafC, at 1,303 to 2,217
GFF4039
GFF4040 - Putative drug efflux protein, at 2,322 to 3,497
GFF4040
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-2
-1
0
1 at 0.456 kb on + strand at 0.456 kb on + strand at 0.457 kb on - strand at 0.594 kb on - strand, within GFF4038 at 0.656 kb on + strand, within GFF4038 at 0.657 kb on - strand, within GFF4038 at 0.702 kb on + strand, within GFF4038 at 0.964 kb on + strand, within GFF4038 at 1.011 kb on - strand, within GFF4038 at 1.011 kb on - strand, within GFF4038 at 1.069 kb on + strand, within GFF4038 at 1.298 kb on + strand at 1.617 kb on + strand, within GFF4039 at 1.701 kb on + strand, within GFF4039 at 1.930 kb on - strand, within GFF4039 at 2.000 kb on - strand, within GFF4039 at 2.067 kb on + strand, within GFF4039 at 2.103 kb on + strand, within GFF4039 at 2.317 kb on + strand at 2.317 kb on + strand at 2.321 kb on + strand at 2.321 kb on + strand at 2.321 kb on + strand at 2.322 kb on - strand at 2.322 kb on - strand at 2.322 kb on - strand at 2.350 kb on + strand at 2.593 kb on - strand, within GFF4040 at 2.594 kb on + strand, within GFF4040 at 2.711 kb on + strand, within GFF4040 at 2.712 kb on - strand, within GFF4040 at 2.712 kb on - strand, within GFF4040 at 2.735 kb on - strand, within GFF4040 at 3.062 kb on - strand, within GFF4040 at 3.192 kb on + strand, within GFF4040
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 456 + -0.0 456 + -0.6 457 - -1.5 594 - GFF4038 0.14 -0.4 656 + GFF4038 0.22 -1.1 657 - GFF4038 0.22 +0.3 702 + GFF4038 0.28 -2.0 964 + GFF4038 0.60 -0.1 1,011 - GFF4038 0.66 -1.8 1,011 - GFF4038 0.66 -0.5 1,069 + GFF4038 0.73 -0.5 1,298 + -0.5 1,617 + GFF4039 0.34 -0.6 1,701 + GFF4039 0.43 -0.5 1,930 - GFF4039 0.69 -1.1 2,000 - GFF4039 0.76 +0.3 2,067 + GFF4039 0.83 -0.7 2,103 + GFF4039 0.87 -1.1 2,317 + -0.9 2,317 + -1.1 2,321 + -0.3 2,321 + +0.4 2,321 + -1.9 2,322 - +0.4 2,322 - +0.3 2,322 - -0.4 2,350 + -0.9 2,593 - GFF4040 0.23 -0.9 2,594 + GFF4040 0.23 +0.4 2,711 + GFF4040 0.33 -0.3 2,712 - GFF4040 0.33 +1.0 2,712 - GFF4040 0.33 +0.9 2,735 - GFF4040 0.35 +1.0 3,062 - GFF4040 0.63 +1.0 3,192 + GFF4040 0.74 -0.5
Or see this region's nucleotide sequence