Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3750 and GFF3751 are separated by 108 nucleotides GFF3751 and GFF3752 are separated by 124 nucleotides GFF3752 and GFF3753 are separated by 151 nucleotides GFF3753 and GFF3754 are separated by 27 nucleotides
GFF3750 - Putative inner membrane protein, at 17,462 to 18,025
GFF3750
GFF3751 - LSU ribosomal protein L25p, at 18,134 to 18,418
GFF3751
GFF3752 - ATP-dependent RNA helicase YejH, at 18,543 to 20,303
GFF3752
GFF3753 - Ribosomal small subunit pseudouridine synthase A (EC 4.2.1.70), at 20,455 to 21,150
GFF3753
GFF3754 - MFS family multidrug transport protein, bicyclomycin resistance protein, at 21,178 to 22,368
GFF3754
Position (kb) 18
19
20
21 Strain fitness (log2 ratio) -2
-1
0
1
2 at 17.547 kb on - strand, within GFF3750 at 17.646 kb on + strand, within GFF3750 at 17.651 kb on + strand, within GFF3750 at 17.763 kb on + strand, within GFF3750 at 17.763 kb on + strand, within GFF3750 at 17.763 kb on + strand, within GFF3750 at 17.833 kb on - strand, within GFF3750 at 17.880 kb on + strand, within GFF3750 at 17.886 kb on + strand, within GFF3750 at 17.978 kb on - strand at 18.024 kb on - strand at 18.024 kb on - strand at 18.552 kb on + strand at 18.553 kb on - strand at 18.700 kb on - strand at 18.735 kb on - strand, within GFF3752 at 18.815 kb on - strand, within GFF3752 at 18.918 kb on + strand, within GFF3752 at 19.397 kb on - strand, within GFF3752 at 19.422 kb on - strand, within GFF3752 at 19.694 kb on + strand, within GFF3752 at 19.694 kb on + strand, within GFF3752 at 19.695 kb on - strand, within GFF3752 at 19.760 kb on + strand, within GFF3752 at 19.819 kb on + strand, within GFF3752 at 19.819 kb on + strand, within GFF3752 at 19.873 kb on - strand, within GFF3752 at 19.873 kb on - strand, within GFF3752 at 19.873 kb on - strand, within GFF3752 at 19.873 kb on - strand, within GFF3752 at 20.013 kb on + strand, within GFF3752 at 20.048 kb on + strand, within GFF3752 at 20.049 kb on - strand, within GFF3752 at 20.098 kb on + strand, within GFF3752 at 20.099 kb on - strand, within GFF3752 at 20.366 kb on + strand at 20.367 kb on - strand at 20.367 kb on - strand at 20.436 kb on + strand at 20.639 kb on - strand, within GFF3753 at 20.639 kb on - strand, within GFF3753 at 20.649 kb on + strand, within GFF3753 at 20.731 kb on + strand, within GFF3753 at 20.818 kb on - strand, within GFF3753 at 20.848 kb on + strand, within GFF3753 at 20.849 kb on - strand, within GFF3753 at 21.079 kb on - strand, within GFF3753 at 21.152 kb on + strand at 21.152 kb on + strand at 21.152 kb on + strand at 21.240 kb on - strand at 21.263 kb on - strand at 21.263 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 17,547 - GFF3750 0.15 +0.7 17,646 + GFF3750 0.33 +0.2 17,651 + GFF3750 0.34 -0.2 17,763 + GFF3750 0.53 +0.5 17,763 + GFF3750 0.53 +0.5 17,763 + GFF3750 0.53 +0.5 17,833 - GFF3750 0.66 +0.2 17,880 + GFF3750 0.74 +0.4 17,886 + GFF3750 0.75 +0.7 17,978 - -0.6 18,024 - -0.1 18,024 - -2.1 18,552 + +0.4 18,553 - -0.2 18,700 - +0.3 18,735 - GFF3752 0.11 +0.4 18,815 - GFF3752 0.15 -0.7 18,918 + GFF3752 0.21 +0.6 19,397 - GFF3752 0.48 -0.1 19,422 - GFF3752 0.50 +1.2 19,694 + GFF3752 0.65 +0.2 19,694 + GFF3752 0.65 -0.5 19,695 - GFF3752 0.65 +1.4 19,760 + GFF3752 0.69 -0.5 19,819 + GFF3752 0.72 -0.2 19,819 + GFF3752 0.72 -0.6 19,873 - GFF3752 0.76 -0.2 19,873 - GFF3752 0.76 +0.3 19,873 - GFF3752 0.76 +1.8 19,873 - GFF3752 0.76 +0.5 20,013 + GFF3752 0.83 +2.0 20,048 + GFF3752 0.85 +0.9 20,049 - GFF3752 0.86 +1.7 20,098 + GFF3752 0.88 -0.3 20,099 - GFF3752 0.88 +0.1 20,366 + +0.2 20,367 - +0.1 20,367 - +0.7 20,436 + +0.3 20,639 - GFF3753 0.26 +0.3 20,639 - GFF3753 0.26 -0.3 20,649 + GFF3753 0.28 +0.0 20,731 + GFF3753 0.40 -0.3 20,818 - GFF3753 0.52 +0.7 20,848 + GFF3753 0.56 +1.2 20,849 - GFF3753 0.57 -0.3 21,079 - GFF3753 0.90 -1.3 21,152 + +0.1 21,152 + +0.2 21,152 + +0.2 21,240 - -0.2 21,263 - -0.6 21,263 - +1.4
Or see this region's nucleotide sequence