Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3094 and GFF3095 are separated by 11 nucleotides GFF3095 and GFF3096 are separated by 299 nucleotides
GFF3094 - D-mannonate oxidoreductase (EC 1.1.1.57), at 102,558 to 104,030
GFF3094
GFF3095 - Uronate isomerase (EC 5.3.1.12), at 104,042 to 105,454
GFF3095
GFF3096 - Putative methyl-accepting chemotaxis protein, at 105,754 to 106,812
GFF3096
Position (kb)
104
105
106 Strain fitness (log2 ratio)
-1
0
1 at 103.066 kb on + strand, within GFF3094 at 103.271 kb on - strand, within GFF3094 at 103.271 kb on - strand, within GFF3094 at 103.300 kb on - strand, within GFF3094 at 103.471 kb on - strand, within GFF3094 at 103.491 kb on + strand, within GFF3094 at 103.511 kb on + strand, within GFF3094 at 103.511 kb on + strand, within GFF3094 at 103.512 kb on - strand, within GFF3094 at 103.811 kb on + strand, within GFF3094 at 103.811 kb on + strand, within GFF3094 at 104.029 kb on - strand at 104.048 kb on - strand at 104.054 kb on - strand at 104.108 kb on + strand at 104.108 kb on + strand at 104.109 kb on - strand at 104.149 kb on - strand at 104.220 kb on - strand, within GFF3095 at 104.222 kb on - strand, within GFF3095 at 104.345 kb on + strand, within GFF3095 at 104.346 kb on - strand, within GFF3095 at 104.367 kb on + strand, within GFF3095 at 104.386 kb on + strand, within GFF3095 at 104.477 kb on - strand, within GFF3095 at 104.698 kb on - strand, within GFF3095 at 104.934 kb on + strand, within GFF3095 at 104.938 kb on + strand, within GFF3095 at 104.960 kb on - strand, within GFF3095 at 104.960 kb on - strand, within GFF3095 at 105.020 kb on - strand, within GFF3095 at 105.025 kb on + strand, within GFF3095 at 105.086 kb on - strand, within GFF3095 at 105.092 kb on + strand, within GFF3095 at 105.196 kb on + strand, within GFF3095 at 105.215 kb on - strand, within GFF3095 at 105.256 kb on - strand, within GFF3095 at 105.304 kb on + strand, within GFF3095 at 105.304 kb on + strand, within GFF3095 at 105.438 kb on - strand at 105.509 kb on + strand at 105.698 kb on + strand at 105.748 kb on - strand at 105.748 kb on - strand at 105.915 kb on + strand, within GFF3096 at 105.945 kb on - strand, within GFF3096 at 106.071 kb on + strand, within GFF3096 at 106.093 kb on - strand, within GFF3096 at 106.200 kb on + strand, within GFF3096 at 106.255 kb on - strand, within GFF3096 at 106.303 kb on - strand, within GFF3096
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 103,066 + GFF3094 0.34 -0.4 103,271 - GFF3094 0.48 -0.3 103,271 - GFF3094 0.48 -1.2 103,300 - GFF3094 0.50 -0.4 103,471 - GFF3094 0.62 +0.3 103,491 + GFF3094 0.63 -0.1 103,511 + GFF3094 0.65 -0.9 103,511 + GFF3094 0.65 +0.3 103,512 - GFF3094 0.65 +0.3 103,811 + GFF3094 0.85 +0.5 103,811 + GFF3094 0.85 +0.3 104,029 - -0.1 104,048 - +0.3 104,054 - +0.1 104,108 + -0.7 104,108 + +1.3 104,109 - +0.3 104,149 - -0.3 104,220 - GFF3095 0.13 +0.5 104,222 - GFF3095 0.13 +0.9 104,345 + GFF3095 0.21 +0.5 104,346 - GFF3095 0.22 +0.5 104,367 + GFF3095 0.23 +0.2 104,386 + GFF3095 0.24 +0.9 104,477 - GFF3095 0.31 +0.3 104,698 - GFF3095 0.46 +0.1 104,934 + GFF3095 0.63 +0.0 104,938 + GFF3095 0.63 -0.1 104,960 - GFF3095 0.65 -0.2 104,960 - GFF3095 0.65 +0.9 105,020 - GFF3095 0.69 -1.3 105,025 + GFF3095 0.70 -0.2 105,086 - GFF3095 0.74 -0.1 105,092 + GFF3095 0.74 -0.8 105,196 + GFF3095 0.82 -0.4 105,215 - GFF3095 0.83 +1.4 105,256 - GFF3095 0.86 +0.2 105,304 + GFF3095 0.89 +0.2 105,304 + GFF3095 0.89 -0.5 105,438 - -0.1 105,509 + -0.0 105,698 + -0.3 105,748 - +0.9 105,748 - +0.1 105,915 + GFF3096 0.15 -0.0 105,945 - GFF3096 0.18 -0.4 106,071 + GFF3096 0.30 -0.2 106,093 - GFF3096 0.32 +0.0 106,200 + GFF3096 0.42 +0.2 106,255 - GFF3096 0.47 +0.9 106,303 - GFF3096 0.52 +0.6
Or see this region's nucleotide sequence