Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3047 and GFF3048 are separated by 28 nucleotides GFF3048 and GFF3049 are separated by 19 nucleotides GFF3049 and GFF3050 are separated by 215 nucleotides GFF3050 and GFF3051 overlap by 1 nucleotides
GFF3047 - Endonuclease I precursor (EC 3.1.21.1) @ Extracellular deoxyribonuclease Dns (EC 3.1.21.-), at 60,539 to 61,246
GFF3047
GFF3048 - Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-), at 61,275 to 62,054
GFF3048
GFF3049 - Glutathione synthetase (EC 6.3.2.3), at 62,074 to 63,021
GFF3049
GFF3050 - UPF0301 protein YqgE, at 63,237 to 63,800
GFF3050
GFF3051 - Putative Holliday junction resolvase YggF, at 63,800 to 64,216
GFF3051
Position (kb)
62
63
64 Strain fitness (log2 ratio)
-2
-1
0
1 at 61.086 kb on - strand, within GFF3047 at 61.143 kb on - strand, within GFF3047 at 61.211 kb on + strand at 61.211 kb on + strand at 61.211 kb on + strand at 61.249 kb on + strand at 61.250 kb on - strand at 61.250 kb on - strand at 61.250 kb on - strand at 61.250 kb on - strand at 61.250 kb on - strand at 61.328 kb on + strand at 61.469 kb on - strand, within GFF3048 at 61.633 kb on + strand, within GFF3048 at 61.634 kb on - strand, within GFF3048 at 61.964 kb on + strand, within GFF3048 at 61.964 kb on + strand, within GFF3048 at 62.047 kb on + strand at 62.182 kb on + strand, within GFF3049 at 62.185 kb on - strand, within GFF3049 at 62.298 kb on + strand, within GFF3049 at 62.359 kb on - strand, within GFF3049 at 62.372 kb on - strand, within GFF3049 at 62.372 kb on - strand, within GFF3049 at 62.503 kb on - strand, within GFF3049 at 62.607 kb on + strand, within GFF3049 at 62.612 kb on + strand, within GFF3049 at 62.613 kb on - strand, within GFF3049 at 63.066 kb on - strand at 63.117 kb on + strand at 63.158 kb on + strand at 63.160 kb on + strand at 63.161 kb on - strand at 63.161 kb on - strand at 63.190 kb on + strand at 63.311 kb on + strand, within GFF3050 at 63.624 kb on + strand, within GFF3050
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 61,086 - GFF3047 0.77 +0.3 61,143 - GFF3047 0.85 -1.0 61,211 + +0.2 61,211 + -0.2 61,211 + +0.3 61,249 + -0.1 61,250 - +0.1 61,250 - -0.3 61,250 - -0.2 61,250 - -0.0 61,250 - -0.2 61,328 + +0.4 61,469 - GFF3048 0.25 +0.5 61,633 + GFF3048 0.46 -0.3 61,634 - GFF3048 0.46 -0.6 61,964 + GFF3048 0.88 +0.5 61,964 + GFF3048 0.88 -0.7 62,047 + +0.7 62,182 + GFF3049 0.11 +0.9 62,185 - GFF3049 0.12 -0.3 62,298 + GFF3049 0.24 -1.2 62,359 - GFF3049 0.30 -0.5 62,372 - GFF3049 0.31 -2.7 62,372 - GFF3049 0.31 +0.1 62,503 - GFF3049 0.45 -1.0 62,607 + GFF3049 0.56 +0.3 62,612 + GFF3049 0.57 +0.0 62,613 - GFF3049 0.57 -0.1 63,066 - -0.7 63,117 + -0.7 63,158 + -0.3 63,160 + -0.0 63,161 - +0.7 63,161 - -0.4 63,190 + -0.3 63,311 + GFF3050 0.13 -1.2 63,624 + GFF3050 0.69 -0.8
Or see this region's nucleotide sequence